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Využití embryotransferu v chovu holštýnského skotu
Musilová, Ivana
This bachelor’s thesis gives an overview and basic information about the method of transferring embryos in cows, basic procedures needed for its realization, physiological processes related to reproduction, and other methods used when working with embryos. It describes the embryo transfer from the farmer’s point of view (such as choosing and preparing the donor cow, collection of embryos and manipulation with them) but also from the animal’s perspective (ovarian and sexual cycle, impregnation, and embryo development). In the practical part, there is an evaluation of data gained over the years 2019 to 2021 from a dairy farm Agras Bohdalov a.s. During the evaluated period there were approximately 57 embryo flushings per year, 442 embryos were collected and 263 of them were suitable for transfer. About 59.3 % of this amount was transferred immediately and the rest was frozen. In total, 268 calves were born from fresh embryos and 67.54 % of them were heifers. There were 179 donor cows chosen and flushed over the three monitored years and 45 % of them were heifers. The most frequently used bulls were Twitch (used 13 times total) and Nighthawk (10 times).
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Biotechnologické metody v reprodukci skotu
Novotná, Eliška
This bachelor thesis deals with biotechnological methods of cattle reproduction. It is a research from academic articles and literature. In the beginning of this thesis, basic information about reproduction, insemination, and its history is described. The history is followed by a description and explanation of individual methods of searching for heat in cattle. Furthermore, this thesis describes various methods of cattle insemination and the use of embryo transfer, which is compared with the insemination itself. Subsequently, methods of gravidity detection and their use are summarised and described. Finally, the information about the insemination of cattle is summarised.
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Principles of ovarian stimulation and oocyte collection in mouse and human.
Klinovská, Karolína ; Hortová, Kateřina (advisor) ; Svoboda, Petr (referee)
This thesis deals with methods of controlled ovarian stimulation and oocyte retrieval on physiological basis. Mouse has been major model organism for wide range of key researches and being able to stimulate its ovarian functions correctly is necessary. That is why mouse stimulation is main topic of this thesis. It summarizes available data and stimulation protocols in relation to factors which can affect the result as a condition, strain, quality of hormones used in administration and others. Potential affections on oocyte and embryo quality are mention according to specific types of administration protocols. Human infertility is slowly becoming common and one of the techniques used to treat it in assisted reproduction is also ovarian stimulation. In the end of this thesis is a chapter dealing with this commonly used method. It is focused on stimulation for oocyte retrieval, as well as on stimulation of recipient mother in embryotransfer. Differences based on hormones, success in In vitro fertilization programme (IVF) and application frequency are mentioned.
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Metody reprodukce využitelné v chovu masného skotu
Střecha, Filip
This thesis entitled "reproduction methods usable in breed of beef cattle" describes the most commonly used reproductive techniques at herds of beef cattle. Breeders implement the bovine reproduction mostly through natural breeding and insemination. The first part of this thesis is devoted to the breeding of bulls, their selection and bonitation. Further, the effect of a bull in a herd is introduced, its classification and the main principles of bull breeding. In the second part the thesis deals insemination. It describes the creation of an insemination dose, its consequent long-term storage and the insemination of cows. The possibilities of influencing the gender and correct timing of insemination are have briefly summarized. The third section introduces the technique of embryo transfer. It describes a stimulation of superovulation, preparation of donors and recipients, flushing of embryos, long-term preservation of embryos and their subsequent transfer. In the fourth section bull catalogues of the Czech Republic, France and Ireland are compared. The individual parts of the cataloques and differences between them are described there. The last part is the assessment of the individual reproductive indicators published in the Czech Republic. The six largest breeds is compared here, and in some consideration beef cattle is compared across all breeds. We evaluates the length of the interim period with respect to the age of a cow, its age at first calving, the percentage of live born of calves and the part of various systems used during ensuring the the reproduction in the herd.
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Porovnání reprodukčních biotechnik využitelných v chovu skotu a faremním chovu jelenů
Kaiserová, Veronika
The bachelor thesis “Comparison of reproductive biotechnology in cattle breeding and deer farms” deals with comparison of biotechnologies used in cattle breeding and deer farms. In the last few years, there has been a growing interest in biotechnology on deer farms and therefore, this work focuses on artificial insemination and embryo transfer. There is a detailed description of methods of heat detection and its synchronization, dia-gnostics of pregnancy, methods of insemination, embryo transfer. In first part there is basic information about female sex organs of the breeding female and hind. Methods of heat detection and their potential use are described as well. The second part is dedicated to insemination, and there are methods which can be used only for one category or both. The third part is devoted to embryo transfer, which is highly developed also in deer. There is a comparison of synchronization protocols for cattle and a comparison of methods of embryo transfer in hind.
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Reprodukční a biotechnické metody využitelné v chovu dojených plemen skotu
Popelková, Markéta
The bachelor thesis is focused on reproductive and biotechnological methods in dairy cattle breeding. In terms of reproductive methods, it deals with natural breeding, its benefits, negatives, selection, housing and treatment of bulls in natural breeding conditions. As for insemination, it is focused on methods, place of sperm deposition, as well as determining the appropriate time for its execution. Another mentioned biotechnological method is embryo transfer, which concentrates on the correct selection of donors, recipients. Embryos can be either retrieved through lavage or the IVF method. It describes the evaluation of embryos, their transmission and also preservation. Both oestrus and ovulation synchronization and its benefits in reproduction are also mentioned. Ovulation synchronization is addressed in more detail in the synchronization programs such as Ovsynch and Presynch. In case of all these methods, both positive and negative aspects are mentioned. The thesis also concentrates on factors that might influence the functionality of the dairy cow's reproductive organs to some extent.
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Biotechnické metody reprodukce skotu
Trávníčková, Barbora
This bachelor thesis is focused on description of basic biotechnological methods used in cattle breeding, their success and comparison in significant world countries. Attention is focused on basic biotechnological methods, such as insemination, embryotransfer (IVP), synchronization, sexing of insemination doses and embryos. In the most commonly used methods in reproduction, insemination describes the methods of detection of rut. Insemination reduces the spread of infection, improves fertility and improves genetic gain. At the end of the thesis, the percentage of success of basic reproductive biotechnology methods and comparison of methods from the economic point of view.
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Principles of ovarian stimulation and oocyte collection in mouse and human.
Klinovská, Karolína ; Svoboda, Petr (referee) ; Hortová, Kateřina (advisor)
This thesis deals with methods of controlled ovarian stimulation and oocyte retrieval on physiological basis. Mouse has been major model organism for wide range of key researches and being able to stimulate its ovarian functions correctly is necessary. That is why mouse stimulation is main topic of this thesis. It summarizes available data and stimulation protocols in relation to factors which can affect the result as a condition, strain, quality of hormones used in administration and others. Potential affections on oocyte and embryo quality are mention according to specific types of administration protocols. Human infertility is slowly becoming common and one of the techniques used to treat it in assisted reproduction is also ovarian stimulation. In the end of this thesis is a chapter dealing with this commonly used method. It is focused on stimulation for oocyte retrieval, as well as on stimulation of recipient mother in embryotransfer. Differences based on hormones, success in In vitro fertilization programme (IVF) and application frequency are mentioned.
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Vliv vybraných způsobů reprodukce ve stádě masného skotu na růst telat
HANŽL, Jaromír
The thesis goal was to evaluate the effect of selected breeding methods in Simmental cattle herd on chosen indicators calf birth weight, weight in the age of 120 and 210 days. The thesis deals with the evaluation of differences between sex and the comparison of calving seasons 2014 and 2015 and also shortly focus on the comparison of chosen sires and the evaluation of its effect on selected indicators. Proposal of measures in specific breeding based collected and statistically evaluated data in part of this thesis. As the first, using a linear model, analysed the effect of individual factors which can affect observed indicators was analysed. Afterwards the evaluation of difference between each factors category was done. Within first observed factor which was breeding method was found, that the best results are reached by embryotransfered calves with birth weight 46.81 kg, weight at 120 days 201.11 kg and weight at 210 days 318 kg. The weight at 210 days within artificial insemination group was only 304.33 kg and within natural breeding group 297.68 kg. Furthermore it was found that bulls reach better results in all observed indicators in comparison with heifers. In the light of birth difficultness is important birth weight which was 46.66 kg within group of bulls and 43.45 kg within group of heifers. Weight at 210 days was 321.59 kg, respectively 294.21 kg in case of heifers. It was also found that in 2015 the studied group of calves reached better results than in year 2014 in all observed indicators. Live weight in 210 days was 326 kg and 295.63 kg respectively. The evaluation of observed indicators according to sires showed that a sire significantly affects birth weight; however the effect was not significant on weight at 210 days. The best of compared sires was ZSI 478, birth weight of calves was 48.8 kg.
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