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Current knowledge and challenges associated with embryo transfer in assisted reproduction in humans and selected mammals.
Hrdová, Lucie ; Komrsková, Kateřina (advisor) ; Cozlová, Nina (referee)
The embryo transfer represents the final and pivotal step of in vitro fertilization, which leads to a successful implantation of the embryo into the uterus and subsequent birth of a healthy offspring if executed correctly. This bachelor thesis focuses on the technique of transfer using a catheter and the potential complications associated with it, including the optimal timing for the procedure. Furthermore, differences in forms of embryo transfer are discussed, assessing advantages and risks, comparing single and multiple, as well as fresh and frozen embryo transfer. The thesis introduces the related topic of cryopreservation and the possibilities in terms of preserving embryos for future use. This thesis also covers hormonal stimulation and uterine response, addressing specifically the role of gonadotropins, steroid hormones, prostaglandins, and the potential positive influence of human growth hormone on optimizing successful outcomes in assisted reproduction. Additionally, possible complications associated with embryo transfer in the early stages of pregnancy, as well as complications that may arise before the actual embryo implantation occurs are covered. Lastly, the issues related to embryo transfer in the context of controlled breeding of selected domesticated animals and endangered mammalian...
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Využití embryotransferu v chovu holštýnského skotu
Musilová, Ivana
This bachelor’s thesis gives an overview and basic information about the method of transferring embryos in cows, basic procedures needed for its realization, physiological processes related to reproduction, and other methods used when working with embryos. It describes the embryo transfer from the farmer’s point of view (such as choosing and preparing the donor cow, collection of embryos and manipulation with them) but also from the animal’s perspective (ovarian and sexual cycle, impregnation, and embryo development). In the practical part, there is an evaluation of data gained over the years 2019 to 2021 from a dairy farm Agras Bohdalov a.s. During the evaluated period there were approximately 57 embryo flushings per year, 442 embryos were collected and 263 of them were suitable for transfer. About 59.3 % of this amount was transferred immediately and the rest was frozen. In total, 268 calves were born from fresh embryos and 67.54 % of them were heifers. There were 179 donor cows chosen and flushed over the three monitored years and 45 % of them were heifers. The most frequently used bulls were Twitch (used 13 times total) and Nighthawk (10 times).
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Biotechnologické metody v reprodukci skotu
Novotná, Eliška
This bachelor thesis deals with biotechnological methods of cattle reproduction. It is a research from academic articles and literature. In the beginning of this thesis, basic information about reproduction, insemination, and its history is described. The history is followed by a description and explanation of individual methods of searching for heat in cattle. Furthermore, this thesis describes various methods of cattle insemination and the use of embryo transfer, which is compared with the insemination itself. Subsequently, methods of gravidity detection and their use are summarised and described. Finally, the information about the insemination of cattle is summarised.
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