|
|
Electrochemical biosensor for the study of DNA methylation
Petrula, Jakub ; Hrdý, Radim (referee) ; Hynek, David (advisor)
This bachelor’s thesis deals with design and optimalisation of custom biosensor for detection of methylated DNA. Teoretical part explains the mechanism and importance of DNA methylation. Next section describes analytical methods used in connection with DNA methylation and some basic direct and indirect methods of detection. Final part is dedicated to experiment itself, which is divided into several sections. Section one deals witch modification of working electrode and optimalisation of detection method. Second section introduces two different ways of DNA methylation detection. First is based on direct detection and second one on detection through the biosensor. Final part shows determination of methylcytosine from sample based on analysing characteristic attributes of signal and numeric algorithm based on curve fitting.
|
|
|
Electrochemical biosensor for glucose detection
Matula, Tomáš ; Majzlíková, Petra (referee) ; Prášek, Jan (advisor)
This thesis pays attention to electrochemical sensors for the detection of glucose. The theoretical part deals with the technology of thick film, their application and firing. It is further described electrochemistry, electrodes used in electrochemistry and analytical methods based on electrochemical reaction. End of theoretical part deals with electrochemical biosensors for detection glucose using special electrodes based on enzyme glucose oxidase. In the experimental part is processed amperometric measure for detection glucose using screenprinted sensors S10 and S8 with immobilized enzyme glucose oxidase. Next was studied effect of plasma, dissolved potassium chloride, storage, ascorbic acid and the reproducibility of the sensors.
|
|
|
A microfluidic platform for working with biological material
Vírostko, Ján ; Švarc, Vojtěch (referee) ; Hrdý, Radim (advisor)
Táto bakalárska práca sa zameriava na návrh a výrobu mikrofluidej platformy určenej pre lýzu buniek a ich následnú analýzu. Táto platforma by mala byť schopná efektívne mechanicky rezať bunky v preparátoch červených krviniek a následne ich analyzovať pomocou dvojelektródového elektrochemického systému. Teoretická časť tejto práce je zameraná na technológiu laboratória na čipe a používané komponenty v tejto technológií, ako napríklad mikrofluidné separátory, mixéry, komory určené na lýzu alebo mikroreaktory, ich aplikáciu a na koniec ich výrobu v čistých priestoroch. Taktiež je časť venovaná stručnej rozprave o fyzike prúdenia tekutín v mikroskopických kanáloch. Praktická časť tejto práce sa skladá z návrhu, výroby v čistých priestoroch a zapúzdrenia troch typov spomínanej platformy pomocou polymeru Parylene C. Následne je popísaná príprava zariadení na testovanie a ich následne testovanie. Na záver je uvedená dokumentácia výroby pomocou vytvárania snímkov s použitím elektrónového mikroskopu a grafické závislosti vypočítané počas charakterizácie elektród a snímky správania sa analytu v komore na lýzu.
|
|
|
Voltammetric Determination of Warfarin at a Screen-Printed Carbon Electrode
Žužičová, Victória ; Vyskočil, Vlastimil (advisor) ; Dejmková, Hana (referee)
In this Bachelor Thesis, the electrochemical behaviour of the drug warfarin sodium was studied at a screen-printed carbon electrode (SPCE) using DC voltammetry (DCV) and differential pulse voltammetry (DPV). The dependence of the voltammetric behaviour of the substance on the pH of the medium was studied in Britton-Robinson buffer (BR buffer) in the pH range of 2.0−12.0. A pH of 7.0 was chosen as the optimum pH for both voltammetric techniques. A repeatability of 3.8% for DCV and 4.3% for DPV was determined for the selected pH. Calibration dependence was monitored under the selected optimal conditions in the concentration range 2∙10−6 to 1∙10−4 mol∙dm−3 . The limit of detection (LOD) was determined to be 1.05∙10−6 mol∙dm−3 for DCV and 2.59∙10−7 mol∙dm−3 for DPV. The limit of quantification (LOQ) was 3.50∙10−6 mol∙dm−3 for DCV and 8.62∙10−7 mol∙dm−3 for DPV. These newly developed methods were used for the determination of the active ingredient in the pharmaceutical formulation Warfarin Orion 5 mg. The values measured by voltammetric methods were compared with those obtained by spectrophotometric measurements. Key words Electrochemistry, Warfarin, Anticoagulants, Direct Current Voltammetry, Differential Pulse Voltammetry, Screen-Printed Carbon Electrode
|
|
|
Electrochemical biosensor for the study of DNA methylation
Petrula, Jakub ; Hrdý, Radim (referee) ; Hynek, David (advisor)
This bachelor’s thesis deals with design and optimalisation of custom biosensor for detection of methylated DNA. Teoretical part explains the mechanism and importance of DNA methylation. Next section describes analytical methods used in connection with DNA methylation and some basic direct and indirect methods of detection. Final part is dedicated to experiment itself, which is divided into several sections. Section one deals witch modification of working electrode and optimalisation of detection method. Second section introduces two different ways of DNA methylation detection. First is based on direct detection and second one on detection through the biosensor. Final part shows determination of methylcytosine from sample based on analysing characteristic attributes of signal and numeric algorithm based on curve fitting.
|
|
|
Electrochemical biosensor for glucose detection
Matula, Tomáš ; Majzlíková, Petra (referee) ; Prášek, Jan (advisor)
This thesis pays attention to electrochemical sensors for the detection of glucose. The theoretical part deals with the technology of thick film, their application and firing. It is further described electrochemistry, electrodes used in electrochemistry and analytical methods based on electrochemical reaction. End of theoretical part deals with electrochemical biosensors for detection glucose using special electrodes based on enzyme glucose oxidase. In the experimental part is processed amperometric measure for detection glucose using screenprinted sensors S10 and S8 with immobilized enzyme glucose oxidase. Next was studied effect of plasma, dissolved potassium chloride, storage, ascorbic acid and the reproducibility of the sensors.
|
guest ::
