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National Repository of Grey Literature 3 records found  Search took 0.00 seconds. 
The comparison of animal species determination (barcoding) methods
 Maťátková, Jana ; Vaněk, Daniel (advisor) ; Sember, Alexandr (referee)
Species identification is the key element in many scientific fields, from diversity studies to forensic science and the food industry. Traditional morphological methods based on the visual distinction of anatomical features often encounter limits associated with distinguishing morphologically similar animals. However, with the rise of molecular methods, a new perspective has emerged, namely DNA analysis, which offers higher accuracy and independence from external factors. This bachelor's thesis presents a detailed overview and a comparsion of various molecular methods used to identify animals. It focuses on a thorough evaluation of the principles of these methods and their effectiveness. Special attention is paid to target sequences associated with DNA barcoding, its universal use and limitations that may arise depending on specific animal species. An important element of successful identification is the existence and use of databases that serve as a source of reference data to compare sequences from unknown samples.
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Molecular markers for species identification of entomopathogenic nematodes (Nematoda: \kur{Steinernematidae})
 FAKTOROVÁ, Lucie
For molecular markers (mitochondrial ND2, ND4, CytB and nuclear ITS) were tested for use in species identification of entomopathogenic nematodes. Markers ND2 and ITS were succesfully amplified and sequenced. These aligned sequences were used for reconstruction of dendrogram and their ability to distinguish species of nematodes was tested by statistical methods.
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Identification of species within \kur{Adelgidae} by molecular markers
 VĚCHTOVÁ, Pavlína
Four molecular markers (nuclear ITS, EF1 alpha and mitochondrial AT-Rich and COI) were tested for use in species identification of Adelgidae family. Markers EF1 alpha and COI were successfuly amplified and sequenced. Aligned sequences of those two markers were used for reconstruction of dendrogram by Minimum Evolution method with Kimura 2-Parameter model. Comparison of dendrogram with assumptions based on morfology and biology of Adelgids confirmed suitability of chosen molecular markers for reliable resolution of taxonomic relationship within Adelgidae, although further testing and markers will be required to delineate sister species with higher precision.
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