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Identification of changes in the behaviour of tumour cells in low concentrations of migrastatics by coherence-controlled holographic microscope
Zábranská, Magdaléna ; Netíková,, Irena Štenglová (referee) ; Veselý, Pavel (advisor)
The subject of the diploma thesis was using of Coherence-Controlled Holographic Microscope in the research of the effect of migrastatics at different concentrations on tumor cells and the interpretation of quantitative phase imaging data. The theoretical introduction is divided into two parts. The first part is devoted to CCHM, which briefly describes the history of holographic microscopy, the principle and a detailed description of the main parts of the microscope. The second part is an introduction to the issue of cancer treatment and the introduction of migrastatic drugs. The experimental part of the work is the analysis of the influence of migrastatics on two tumor lines (H1299 and HT1080) from the measured time-lapse records taken by a holographic microscope. The effect of drugs was evaluated using characteristics describing morphological and dynamic cell changes in the SophiQ software. The data were then graphically and statistically processed to compare and evaluate the effect of migrastatics at low levels. In conclusion of the work, the findings were summarized and selected characteristics were proposed to analyze the impact of migrastatics.
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Identification of changes in the behaviour of tumour cells in low concentrations of migrastatics by coherence-controlled holographic microscope
Zábranská, Magdaléna ; Netíková,, Irena Štenglová (referee) ; Veselý, Pavel (advisor)
The subject of the diploma thesis was using of Coherence-Controlled Holographic Microscope in the research of the effect of migrastatics at different concentrations on tumor cells and the interpretation of quantitative phase imaging data. The theoretical introduction is divided into two parts. The first part is devoted to CCHM, which briefly describes the history of holographic microscopy, the principle and a detailed description of the main parts of the microscope. The second part is an introduction to the issue of cancer treatment and the introduction of migrastatic drugs. The experimental part of the work is the analysis of the influence of migrastatics on two tumor lines (H1299 and HT1080) from the measured time-lapse records taken by a holographic microscope. The effect of drugs was evaluated using characteristics describing morphological and dynamic cell changes in the SophiQ software. The data were then graphically and statistically processed to compare and evaluate the effect of migrastatics at low levels. In conclusion of the work, the findings were summarized and selected characteristics were proposed to analyze the impact of migrastatics.
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Topical and systemic treatment of acne vulgaris
Ackermannová, Veronika ; Hrdina, Radomír (advisor) ; Vopršalová, Marie (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Pharmacology and Toxicology Student: Veronika Ackermannová Supervisor: prof. Radomír Hrdina, MD, CSc. Title of diploma thesis: Topic and systemic treatment of acne vulgaris Acne vulgaris is a skin disease affecting the hair follicles and sebaceous glands. The disease manifests itself by increased sebum production, non-inflammatory (comedones) and inflammatory lesions (papules, pustules, nodules, cysts). It occurs predominantly in adolescents, but may persist into adulthood. It is a multifactorial disease, which is caused by several factors (internal and external stimuli). The major pathogenetic factors include increased sebum production, hyperkeratosis, P. acnes colonization and inflammation present. First, it is necessary to diagnose the type of acne in order to choose the right and effective therapy, because there is not only one type of acne. There are many types and variants of acne, and although they show similar symptoms (affecting the follicles of sebaceous glands), their cause often differs. There is no uniform classification system for acne vulgaris and it varies between authors. Some authors classify acne vulgaris according to severity into mild, moderate and severe, others into comedonic, papulopustular, nodulocystic...
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