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Somatic embryogenesis of conifers: description of structural development
Kadlecová, Marie ; Albrechtová, Jana (advisor) ; Konrádová, Hana (referee)
Somatic embryogenesis is a very important tool of in vitro cultivation, therefore, it has recently devoted adequate attention. Stages of somatic embryogenesis involve several consecutive steps: induction of embryogenic culture and its proliferation, maturation of embryo, desiccation, germination and subsequent transfer of embryos into young seedlings. All of these steps are directly influenced by many factors, such as a choice of the original explant, time of cultivation, physical conditions or the composition of the culture medium. There are still emerging new studies describing how the culture conditions and, thus, the quality of embryos could be improved. An important prerequisite for further improving the quality of somatic embryos is good knowledge of aculture protocol, i.e. right concentrations of amended substances in the culture medium, the timing of each step, using the suitable explants etc. Another very important thing is good knowledge of development of all anatomical structures established during embryogenesis. Only then it will get closer to the point when somatic embryos will not arise only in the laboratory, but could be successfully used in practice. The object of this work is to summarize existing knowledge about development of all anatomical structures during somatic...
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