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Microalgae harvesting
Drozd, Jiří ; Naď, Martin (referee) ; Lošák, Pavel (advisor)
This work describe microalgae and their properties. Within the scope of the work, a research was conducted on the utilized microalgae and the possibilities of cultivation in the Central European region were examined. Furthermore, harvesting and drying methods for microalgae were analyzed, and an experiment was conducted using a promising spray drying method. The research revealed the properties and potential uses of microalgae. Various types of microalgae, their nutritional values, and possibilities of application in a wide range of fields were investigated. The cultivation possibilities of microalgae in the Central European region, including the utilization of wastewater and renewable energy sources, were also examined. Harvesting and drying methods for microalgae were analyzed, considering the advantages and disadvantages of different techniques such as flocculation, centrifugation, and spray drying. An experiment utilizing the spray drying method was conducted, and the results showed that this method is effective and enables the achievement of high quality and purity of dried microalgae. The work also explores the possibilities of further development and utilization of microalgae. It describes the potential use of microalgae as food supplements and as alternatives to traditional protein sources. Additionally, the potential applications of microalgae in the pharmaceutical and cosmetic industries were mentioned. In conclusion, this work provides information on the utilization of microalgae and the methods of cultivation, harvesting, and drying. The results suggest that microalgae have great potential for further development and utilization in various industries.
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The influence of Biochar Cultivation in Soil on its Physicochemical Characteristics
Hladký, David ; Sovová, Šárka (referee) ; Kalina, Michal (advisor)
This bachelor thesis is focused on the effect of soil factors on the physical-chemical characteristics of biochar. It describes the change in biochar after a long-term cultivation experiment, as well as, sequential leaching that was implemented on a shorter time scale. The theoretical part describes individual physical-chemical characteristics, structure, possible applications and optimal methods of biochar characterization. In the thesis, we have learnt why biochar is called a soil conditioner, not a fertilizer. The experimental part deals with the study of changes in the physicochemical characteristics of biochar after the cultivation experiment. These results are compared with the sequential leaching of biochar in selected media (water, citric acid, hydrogen peroxide) that simulate the effects that can act on biochar in soil. The experimental results show the similarity of individual observed values of the samples after the long-term cultivation experiment and sequential extractions. It can be seen that the alkaline nature of biochar is caused by salts of alkali metal and alkaline earth metal. Mostly due to the presence of potassium in the biochar structure and then also the presence of calcium and sodium. These ions are also the explanation of the high conductivity, which together with pH, decreases significantly after five leaching cycles, and stabilize after a two-year cultivation period. All biochars samples used in the work have a high content of organic matter, which is a optimal prerequisite for the use in agriculture and the improvement of soil properties.
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Microbial production of lipids and their applications
Kročianová, Natália ; Szotkowski, Martin (referee) ; Němcová, Andrea (advisor)
The aim of this bachelor's thesis was the production of lipids by selected strains of yeasts of the Metschnikowia genus using food production substrates. Produced microbial lipids are nowadays highly valued and sought after mainly in the biotechnology industry as a substitute for carbon in the industrial process of biofuel production. Plant, ecological, and cost-effective substrates were used for cultivation ; the influence of cultivation conditions on the growth of yeast was monitored at laboratory and reduced temperature. Four types of yeast were investigated, namely M. pulcherrima 147, M. pulcherrima 149, M. sinensis 1144, and M. zizyphicola 1147. The produced biomasses were analyzed using gas chromatography; in analyses, individual fatty acid composition was determined. Visible changes could be observed at different cultivation temperatures and used substrates. The best results were achieved by substrates that contained Metschnikowia sinensis 1144, while all selected yeasts were capable of lipid production. The highest representation of polyunsaturated fatty acids was demonstrated in each analyzed yeast.
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Optimization of culture conditions of selected {E.coli} strains producing recombinant proteins
MICHALCOVÁ, Zuzana
Escherichia coli is one of the most preferred organisms to produce recombinant proteins. E. coli is a well-established host that offers ease of genetic manipulation, short culture times, continuous fermentation capability and affordable culture media. To achieve high levels of protein expression, and therefore high recombinant protein production, optimization of culture conditions is required. This thesis is focused on the optimization of cultivation, the practical part is focused on the production of Uridine Phosphorylase (UP) and Purine Nucleoside Phosphorylase (PNP) enzymes. E. coli bacteria producing UP or PNP were cultured on six media with different nutrient sources. Protein production was verified on a spectrophotometer by the Bradford assay and further by polyacrylamide gel electrophoresis with SDS (SDS-PAGE). Enzyme activity was measured by HPLC assay. In addition, the dry weight biomass was determined for all samples. Based on results from these assays, Terrific Broth medium with glucose addition after 4 h of cultivation (TBG4) was selected and subsequently used for upscaling to the fermenter. Successful cultivation in the fermenter confirmed the results of cultivation in Erlenmeyer flasks.
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Determination of plants of the Bromeliaceae family and their growing in the Botanical garden in Tábor
PECHAČOVÁ, Johana
This diploma thesis deals with determining plants from the Bromeliaceae family in the Botanical Garden in Tábor. The collection of bromeliads in the original greenhouse has not yet been completely determined. Due to the overall reconstruction of the garden planned for 2024, the original greenhouse will also be reconstructed, and a new exhibition of plants of the Bromeliaceae family will be created here, in which their determination and proposal for cultivation according to the outputs of this diploma thesis will be applied. With the help of professional literature and articles, their determination, evaluation of their health status, and a proposal for improving the conditions for their cultivation in the newly reconstructed greenhouse of the Tábor Botanical Garden will be provided.
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Effect of sowing way on production parameters of spring poppy
ROTHBAUER, Petr
This diploma thesis compares the influence of different methods of poppy seeding on its production and quality parameters. The evaluation was based on a literature search and an operational trial, where the spring poppy variety MS Harlekýn was sown in different ways. The final results did not show a significant difference in poppy parameters between sowing with harrows and without harrows. Poppy sown with harrows achieved a real yield of 1.34 t/ha, poppy sown without harrows achieved a real yield of 1.12 t/ha. In the conclusion, findings and recommendations based on the results of the experiment are summarized.
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Pěstování a odrůdový sortiment papriky roční
Suvorova, Mariya
Suvorova, M., Cultivation and variety of annual peppers. Bachelor thesis. Brno: Mendel University in Brno, 2021. The bachelor's thesis deals with the cultivation of a selected assortment of annual pepper (Capsicum annuum L.). The aim of the thesis is to describe the growing technology and agricultural technology, including protection against diseases and pests and to determine the growth and yield indicators for a selected range of annual peppers. The first part of the thesis is focused on the elaboration of a literature review related to the origin of the species, botanical characteristics, habitat requirements, cultivation technology and assortment of varieties. The second part contains a description of an experimental experiment in the cultivation of peppers with subsequent measurement and evaluation of yield indicators. The results obtained during the experiment were tabulated and explained. The final part of the thesis is devoted to the discussion of the results and comparison with the results from previous years.
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Možnosti pěstování rostlin rodu konopí
Zapalač, Radek
Bachelor thesis entitled Cultivation possibilities of Cannabis genus plants in the theoretical part deals with botanical characteristics, morphology, anatomy, content compounds and cultivation legislation of Cannabis. In the practical part the thesis deals with an experiment on comparing the influence of various parts of the light spectrum on biosynthesis of phytocannabinoids in Cannabis sativa L. plants cultivated in controlled conditions in the biotechnology pavilion of Mendel University in Brno.
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