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Structure-modelling studies of the haloalkane dehalogenase LinB
ŘEŽÁBEK, Josef
The main aim of this bachelor thesis was structure-dynamics study of LinB86 mutant variant protein isolated from the soil bacterium Sphingobium japonicum UT26. Standard and advanced protein crystallization techniques, basis of solving protein structures from diffraction data and study of enzyme structure and dynamics with the use of standard SW for molecular modelling were applied.
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Biodegradation environmental pollutans - characterization of mutant haloalkan dehalogenase DhaA31 of Rhodococcus rhodochrous NCIMB 13064
MALCHER, Pavel
The project was focused on using of protein crystallography practically and mainly on preparation of crystals of model protein Thermolysin and haloalkan dehalogenase mutant variant DhaA31 with the use of standard and advanced crystallization methods. Later the DhaA31 structure was solved and graphically visualized using the molecular modeling programs as well as the structure of Thermolysin. Obtaining the crystals of both studied proteins and description of the DhaA31 structure, active site and dehalogenation reaction were results of the project. DhaA31 mutant variant was prepared to increase the catalytic reaction during degradation of halogenated compounds such as 1,2,3-trichloropropane (TCP) from water sources.
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Kinetic behavior of the NAD(P)H:Quinone oxidoreductase WrbA from Escherichia coli.
KISHKO, Iryna
This Ph.D. thesis addresses the structure-function relationship of the multimeric oxidoreductase WrbA with the principal aim being the explanation of the unusual kinetics of this enzyme in molecular terms, and thus getting an insight about its physiological role in bacteria. WrbA is a multimeric enzyme with FMN as a co-factor, catalyzing the oxidation of NADH by a two electrons transfer. Structure and function analysis of WrbA places this enzyme between bacterial flavodoxins and eukaryotic oxidoreductases in terms of its evolutionary relationship. The kinetic activity of WrbA was studied under varying conditions such as temperature, pH etc, and its kinetic mechanism was evaluated from parameters KM and Vmax and confirmed by product inhibition pattern experiments. Crystallization and proteolytic experiments also underpin the functional importance of the multimeric nature of WrbA and aid the understanding of the physiological role of this enzyme in molecular terms.
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Crystallization of ammonium aluminium sulphate
Hostomský, Jiří ; Hostomská, Věra
The report deals with research and development of the process for production of extra pure ammonium aluminium sulphate by crystallization from solution. In the pilot plant, the crystallization experiments were carried out with the solution volume of 50 litres. The distribution of contaminants (Na, K, Fe, Si, Ca, Cr, Mg, Ti, Ga, Mn, Zn, Co, Pb a Cu) between the crystalline phase and solution was determined. The heat balance of the crystallization process was evaluated.
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Crystallization of ammonium aluminium alum
Hostomský, Jiří ; Hostomská, Věra
The report deals with the research and development of the process for production of extra pure ammonium aluminium sulphate by crystallization from solution. The distribution of contaminants (Na, K, Fe, Si, Ca, Cr, Mg, Ti, Ga, Mn, Zn, Co, Pb a Cu) between the crystalline phase and solution was determined for two initial concentrations of ammonium aluminium sulphate and for two final crystallization temperatures. The effect of recrystallization of ammonium aluminium sulphate and the effect of the excess of ammonium ions upon the product purity was investigated.
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Research and development of the process for production of extra pure ammonium aluminium sulphate II
Rodová, A. ; Hora, L. ; Bezucha, P. ; Synek, V. ; Kokšal, J. ; Hodslavská, J. ; Hostomský, Jiří ; Hostomská, Věra
295 Research and development of the process for production of extra pure ammonium aluminium sulphate, where the concentration of contaminants does not exceed 0.3 ppm for Ca, Co, Cr, Fe, Mg, Mn, Pb, Si, Ti, Zn, 2.0 ppm for Ga and Na and 3.0 ppm for K. Preparation of ammonium aluminium sulphate (alum) solution by dissolution of aluminium hydroxide in sulphuric acid solution and by addition of ammonia. Purification of alum by crystallization and recrystallization.
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