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Development of voltammetric methods for detection of bile acids and their conjugates
Petráňová, Karolína ; Schwarzová, Karolina (advisor) ; Nesměrák, Karel (referee)
The aim of this thesis is to study the electrochemical properties of bile acids and their conjugates. Specifically, cholic acid, chenodeoxycholic acid, taurocholic acid, taurochenodeoxycholic acid, glycocholic acid, and glycochenodeoxycholic acid were studied. The measurements were carried out on a boron-doped diamond electrode in an environment of acetonitrile and perchloric acid, with a water content of 0.55% in the solution by method of cyclic voltammetry. In order for bile acids to be electrochemically active, they must first be dehydrated, which takes place in a reaction with perchloric acid. This reaction requires a relatively long time, so it was accelerated by heating the compounds. The goal of this work was to determine the conditions under which the dehydration products of bile acids can be detected. The water content that can be added to the solution after dehydration to keep the voltammetric signal stable was determined. Furthermore, it was found that the voltammetric determination can be carried out even at a pH that is not extremely acidic in which is the the dehydration carried out. Finally, calibration dependencies were compiled.
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