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Analysis of selected nutritional parameters in the new varieties of gooseberries
Nováková, Jana ; Vítová, Eva (referee) ; Vespalcová, Milena (advisor)
Diploma thesis deals with the new varieties of gooseberries. The theoretical part characterizes gooseberry from a biological point of view, what is the content of healthful nutrients and states food technology aspects. Furthermore, the theoretical part deals with the main parameters examined nutrition: vitamin C, anthokyanům and antioxidant activity. In the experimental part was measured both weight solids content and soluble solids. Vitamin C was measured by HPLC, anthocyanins using a pH differential method and for antioxidant activity determination was selected method by Kanedy known also as DPPH.
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Problems of phenols in water
Tylichová, Petra ; Čáslavský, Josef (referee) ; Doležalová Weissmannová, Helena (advisor)
This bachelor`s thesis deals with the issue of phenols in the waters. The theoretical part summarises the information about the characteristics of the phenols, its use, appearance in the environment, and its effects on human health. There are described methods of determination these substance in the waters. The last chapter disserts on possible methods of eliminating the phenols from the aqueous environment with special attention paid to the adsorption method. Part of this thesis is also dedicated to the chlorinated phenols which originate from chlorination of the phenolic waters and which cause the sensory interferences of the waters. The experimental part of this thesis is dedicated to removing the phenol from a model water through the adsorption into a powdery active carbon Norit SA SUPER.
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Hormones and their presence in environmental
Sučková, Tereza ; Dvořáková, Petra (referee) ; Vávrová, Milada (advisor)
This thesis deals with the issue of hormones in the environment and analytical methods for the determination of hormonal substances in water and soil ecosystems. The thesis includes an overview of important hormone groups (divided into categories according to their chemical structure) and basic information about properties of chosen hormones, their sources and impacts on the environment. The next focuses upon methods of determination of hormones in soil and water ecosystems. Finally, a standard operational manual for analysis of estrogens in surface water has been created and is provided herein.
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Positive effects of conjugated linoleic acid on human health
Baštová, Daniela ; Vespalcová, Milena (referee) ; Vítová, Eva (advisor)
This bachelor thesis deals with the characteristics of the fatty acids, their structure, nomenclature, properties, reactions and analysis. It is also dedicated to the history, sources, effects on human health and the correct ratio of omega-fatty acids in the body. The main issue of this work is conjugated linoleic acid, which is known to its countless positive effects, which can include weight loss and lower level of cholesterol, anticarcinogenic effects, prevention of osteoporosis and arteriosclerosis, etc. The work is focused on its characteristics, structure, occurrence in food, chemical production and a wide range of preparations available on the market. Last but not least, deals with the possibilities of its determinations, including gas chromatography, high performance liquid chromatography, gas chromatography-mass spectrometry, NMR spectroscopy, and combined methods.
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Substituted polyaromatic compounds in environmental components
Kubalík, David ; Mravcová, Ludmila (referee) ; Vávrová, Milada (advisor)
This work is focused on substituted polycyclic aromatic hydrocarbons (PAHs) in environmental compartments. The thesis examined the development of these compounds and their entry into the ecosystem. Due to their proven negative characteristics to be addressed also their toxicology. The next section discusses the appropriate methods, including sampling, sample preparation and final determination. The conclusion of this work forms draft standard operating procedure for their determination.
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Diagnostics Methods of Dissolved Gas in Transformer Oil Observation
Hindra, Matěj ; Janda, Ondřej (referee) ; Ministr, Martin (advisor)
This thesis is devoted to analysis of the diagnostic methods used in practice. It is divided into two parts: theoretical and practical. The theoretical part concerns with the general description of the transformers. Further it provils informatik about systems for sampling oil from transformers with insulating oil – paper system. Another important part is the description of gas chromatography and TRANSPORT X. Description of the most appropriate evaluation methods for assessment of the state of the transformer is included as well.
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Quantitative analysis of tyrosine kinase inhibitors employing liquid chromatography with mass spectrometry detection
Maier, Jan ; Pávek, Petr (advisor) ; Lochman, Lukáš (referee)
The submitted thesis is devoted to the quantitative analysis of tyrosine kinase inhibitors, specifically imatinib and nilotinib, by liquid chromatography-mass spectrometry method. The main purpose of developing this new method of analysis at the Department of Clinical Biochemistry and Diagnostics at the University Hospital in Hradec Králové was measuring and monitoring serum or plasma concentration levels of these drugs in patients with chronic myeloid leukemia, less often in patients with gastrointestinal stromal tumour. The main task during the elaboration of the thesis was to fully optimize and validate the method. Previously, this method for the analysis of tyrosine kinase inhibitors was routinely performed here by high-performance liquid chromatography with spectrophotometric (UV) detection. As part of the modernization of laboratory technology, they started to use high-performance liquid chromatography with mass spectrometry at the workplace. The analytes with their internal standards were obtained by a liquid-liquid extraction process. Then, samples were separated on a C18 reverse phase column using isocratic elution. Subsequently, both analytes were detected by a triple quadrupole tandem mass spectrometer with ESI ion source in a positive mode. As a part of the method validation was to...
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Studium těkavých látek v pivovarských surovinách, v meziproduktech při výrobě piva a v pivu
Kleinová, Jana
Gas chromatography with mass spektrometry and gas chromatography with flame ionization detector were used for the determination of volatile compounds in raw material for beer production, in intermediate products and in beer. Isolation of compounds with low boiling point was optimized using purge and trap analysis, solid phase microextraction, liquid extraction, and static headspace. Solid phase microextraction was proved to be the most useful for determination of Maillard reaction products that are formed during kilning and roasting of malt, hop essential oils and volatile substances produced by fermentation. The ratio of hop essentials oils with lower boiling point (alfa-humulene, beta-farnesene and beta-caryophyllene) decrease during wort boiling. Maillard reaction products are formed during wort boiling, although the content of 3-methylbutanal and maltol is reduced by evaporation. Higher concentration of volatile substances contained top-fermented beer except 3-methyl-1-butanol. The Dudák Premium proved the best results of the analyzed bottled beers. The Stella Artois contained the lowest concentration of volatile compounds.
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Zavedení a optimalizace metodiky pro stanovení lipofilních a hydrofilních vitamínů v biologických vzorcích
Kalábová, Vendula
The main scope of this diploma thesis are the methods used for building vitamins, especially priorizing chromatographic methods. You can also find the characteristics of vitamins dissolvable in fat and water, their physiological meaning, daily use, presence in foodstuffs and methods, which are suitable for their analysis. The experimental part describes the sample preparation, their vitamin extraction methods, the requierements for chromatographic assessions, methods of qualitative and quantitative evaluation of chromatographs with the focus on the calibration curve method. The work also puts an emphasis on establishing the limit of detection and the limit of quatification, and determining the lipophilic vitamins in matrices rich of these vitamins using the method of HPLC.
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