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Chiral analysis of drug residuals in waste waters
Svobodová, Dagmar ; Opatřilová, Radka (referee) ; Čáslavský, Josef (advisor)
The theoretical part shortly describes chirality with focus on chiral pharmaceuticals. The processes of their absorption, distribution, metabolism and elimination in human body are discussed. These points are very important to understand possible fate of chiral drugs in the environment as there is only little data concerning their environmental behaviour. The occurrence and enantioselective toxicity of chiral drugs is also discussed here. One of the chapters describes non-steroidal anti-inflammatory drugs, as they are analyzed in the wastewater in the experimental part, and their occurrence in the environment. The experimental part describes optimization of the enantioselective HPLC method using Chiralpak AD as column for ibuprofen and ketoprofen. Reproducible separation of enantiomers wasn’t achieved for naproxen. Optimized methods were then applied for analysis of samples from municipal wastewater treatment plant in Brno-Modřice.
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Optimization and validation of analytical method for determination of selected drugs
Geryk, Radim ; Tesařová, Eva (advisor) ; Janečková, Lucie (referee)
The aim of this work was finding suitable conditions for separation of enantiomers of citalopram and citadiol, optimization of chromatographic system and validation of the analytical method for determination of selected enantiomers in drugs. Drugs are often chiral compounds. In many cases, only enantiomer has required pharmacological effects. Advanced chemical technologies associated with the synthesis, separation and analysis of the individual enantiomers caused an increase of the number of new chiral drugs in the form of single pure enantiomers, the chiral switch. Citalopram is one of the widely used antidepressants of selective serotonin reuptake inhibitors (SSRI). Citalopram is used for treatment of depression, panic anxienty or obsessive compulsive disorder of pathological laughing and crying. The pharmacological activity is associated with the S-citalopram, while R-enantiomer is essentially inactive and even counteracts the activity of escitalopram. Citadiol is a chiral syntetic precursor of citalopram. This thesis was focused on monitoring enantioseparation "behaviour" of selected enantiomers. HPLC method with chiral stationary phases based on macrocyclic antibiotics, cyclofructans and cellulose was applied for enantioseparation of the above-mentioned compounds. The optimized chromatographic...
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Determination of enantiomers of selected drugs by HPLC
Geryk, Radim ; Tesařová, Eva (advisor) ; Chudoba, Richard (referee)
Drugs are often chiral compounds. In many cases only one of the enantiomers has required therapeutical effects. Citalopram belongs to a group of chiral drugs. This drug is used as an antidepresivum for treating anxiety, fear or suppressing of pain feeling. Its synthetical precursor is named citadiol and this compound is also chiral. HPLC method with chiral stationary phases based on macrocyclic antibiotics was applied for enantioseparation of above-mentioned compounds. Polar-organic and ionic-polar separation modes were found to be suitable for chiral separation of citalopram and citadiol enantiomers. The aim of this work was monitoring of enantioseparation "behaviour" of selected enantiomers and to find suitable separations conditions using HPLC method.
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Optimization and validation of analytical method for determination of selected drugs
Geryk, Radim ; Tesařová, Eva (advisor) ; Janečková, Lucie (referee)
The aim of this work was finding suitable conditions for separation of enantiomers of citalopram and citadiol, optimization of chromatographic system and validation of the analytical method for determination of selected enantiomers in drugs. Drugs are often chiral compounds. In many cases, only enantiomer has required pharmacological effects. Advanced chemical technologies associated with the synthesis, separation and analysis of the individual enantiomers caused an increase of the number of new chiral drugs in the form of single pure enantiomers, the chiral switch. Citalopram is one of the widely used antidepressants of selective serotonin reuptake inhibitors (SSRI). Citalopram is used for treatment of depression, panic anxienty or obsessive compulsive disorder of pathological laughing and crying. The pharmacological activity is associated with the S-citalopram, while R-enantiomer is essentially inactive and even counteracts the activity of escitalopram. Citadiol is a chiral syntetic precursor of citalopram. This thesis was focused on monitoring enantioseparation "behaviour" of selected enantiomers. HPLC method with chiral stationary phases based on macrocyclic antibiotics, cyclofructans and cellulose was applied for enantioseparation of the above-mentioned compounds. The optimized chromatographic...
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Determination of enantiomers of selected drugs by HPLC
Geryk, Radim ; Chudoba, Richard (referee) ; Tesařová, Eva (advisor)
Drugs are often chiral compounds. In many cases only one of the enantiomers has required therapeutical effects. Citalopram belongs to a group of chiral drugs. This drug is used as an antidepresivum for treating anxiety, fear or suppressing of pain feeling. Its synthetical precursor is named citadiol and this compound is also chiral. HPLC method with chiral stationary phases based on macrocyclic antibiotics was applied for enantioseparation of above-mentioned compounds. Polar-organic and ionic-polar separation modes were found to be suitable for chiral separation of citalopram and citadiol enantiomers. The aim of this work was monitoring of enantioseparation "behaviour" of selected enantiomers and to find suitable separations conditions using HPLC method.
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|
|
Chiral analysis of drug residuals in waste waters
Svobodová, Dagmar ; Opatřilová, Radka (referee) ; Čáslavský, Josef (advisor)
The theoretical part shortly describes chirality with focus on chiral pharmaceuticals. The processes of their absorption, distribution, metabolism and elimination in human body are discussed. These points are very important to understand possible fate of chiral drugs in the environment as there is only little data concerning their environmental behaviour. The occurrence and enantioselective toxicity of chiral drugs is also discussed here. One of the chapters describes non-steroidal anti-inflammatory drugs, as they are analyzed in the wastewater in the experimental part, and their occurrence in the environment. The experimental part describes optimization of the enantioselective HPLC method using Chiralpak AD as column for ibuprofen and ketoprofen. Reproducible separation of enantiomers wasn’t achieved for naproxen. Optimized methods were then applied for analysis of samples from municipal wastewater treatment plant in Brno-Modřice.
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