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Preparation of affinity surfaces for clinical diagnostics
Kalaninová, Zuzana ; Pompach, Petr (advisor) ; Žáček, Petr (referee)
Botulinum toxins are group of protein venoms produced by Clostridium bacteria. These toxins belong to the most toxic substances causing paralytic disease called botulism. The endopeptidase activity of the toxins leads to the blockage of neurotransmitters release to neural junctions. Since the first symptoms of botulism are observed within 48 hours and toxin remains active for months, botulinum toxins are considered as potential bio- terrorism agents. For successful treatment, fast assays for detection of the toxin presence and type are highly required. This work presents a new method that could be potentially used for determination of botulotoxin in various matrices. The method is based on the detection of products of botulinum toxin A (BoNT/A) endopeptidase activity by affinity surfaces prepared by ambient ion soft landing and MALDI mass spectrometry. For immobilization and subsequent enrichment of biotinylated products of BoNT/A activity, proteins with high affinity to biotin - avidin, neutravidin and streptavidin - were used. The presented method, which combines affinity surfaces with MALDI mass spectrometry is sensitive, robust and fast and might be potentially used for detection of BoNT/A activity. Key words: ambient ion soft landing, neutravidin, botulinum toxin A, BoNT/A, MALDI/TOF mass...
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Effect of Botulinum toxin on muscular hyperactivity and motor performance of the patients
Kratochvílová, Pavla ; Kraus, Josef (advisor) ; Syslová, Jana (referee)
This study deals with the effects of botulinum toxin A treatment on pathological muscular hyperactivity in a group of 53 children. The thesis objective was to evaluat eeffect of botulinum toxin on muscle tone, range of movement and motor performance of the patient. The theoretical part summarizes the knowledge about this theme, characterizes the term spasticity and an impact of botulinum toxin on abnormal muscular hyperactivity. The practical part evaluates the effect of botulinum toxin in two groups of patients. The first group consists of 14 children, who were tested before and at least one month after botulinum toxin application. Clinical assessment involved range of movement measurement, the evaluation of spasticity with Modified Ashworth scale, Global Assesment of Spasticity Scale (GSA), assessment of motor performance with the use of GMFM test and Functional Independence Meassure for children WeeFIM and summarized physical therapeutic methods used in treatment of patients before and after the application. The second group consists of 39 patients who administered the GSA survey forms within a period of one month. The results confirmed positive effect of botulinum toxin treatment on reduction of spasticity, increasing of range of movement and improvement of motor performance of children. Powered by...
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Preparation of affinity surfaces for clinical diagnostics
Kalaninová, Zuzana ; Pompach, Petr (advisor) ; Žáček, Petr (referee)
Botulinum toxins are group of protein venoms produced by Clostridium bacteria. These toxins belong to the most toxic substances causing paralytic disease called botulism. The endopeptidase activity of the toxins leads to the blockage of neurotransmitters release to neural junctions. Since the first symptoms of botulism are observed within 48 hours and toxin remains active for months, botulinum toxins are considered as potential bio- terrorism agents. For successful treatment, fast assays for detection of the toxin presence and type are highly required. This work presents a new method that could be potentially used for determination of botulotoxin in various matrices. The method is based on the detection of products of botulinum toxin A (BoNT/A) endopeptidase activity by affinity surfaces prepared by ambient ion soft landing and MALDI mass spectrometry. For immobilization and subsequent enrichment of biotinylated products of BoNT/A activity, proteins with high affinity to biotin - avidin, neutravidin and streptavidin - were used. The presented method, which combines affinity surfaces with MALDI mass spectrometry is sensitive, robust and fast and might be potentially used for detection of BoNT/A activity. Key words: ambient ion soft landing, neutravidin, botulinum toxin A, BoNT/A, MALDI/TOF mass...
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The influence of botulotoxin application in lower extremities on physiotherapy of children with a spastic form of infantile cerebral palsy
Kratochvílová, Pavla ; Zounková, Irena (advisor) ; Prajerová, Hana (referee)
Cerebral Palsy is one of the most frequent neurodevelopmental illnesses occuring in the child age. Main manifestations of the illness include a disorder of muscle tone leading to the development of spasticity. One of contemporary methods of Cerebral Palsy's therapy has been local application of Botulinum Toxin A. This toxin effects a blockade of neuromuscular transmission on neuromuscular junction and in short term limits excessive contractions of spastic muscles. This thesis deals with the influence of physical therapy of children suffering from Cerebral Palsy following the application of Botulotoxin A and compares physical therapic methods applied in the Czech Republic and abroad. Powered by TCPDF (www.tcpdf.org)
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Effect of Botulinum toxin on muscular hyperactivity and motor performance of the patients
Kratochvílová, Pavla ; Syslová, Jana (referee) ; Kraus, Josef (advisor)
This study deals with the effects of botulinum toxin A treatment on pathological muscular hyperactivity in a group of 53 children. The thesis objective was to evaluat eeffect of botulinum toxin on muscle tone, range of movement and motor performance of the patient. The theoretical part summarizes the knowledge about this theme, characterizes the term spasticity and an impact of botulinum toxin on abnormal muscular hyperactivity. The practical part evaluates the effect of botulinum toxin in two groups of patients. The first group consists of 14 children, who were tested before and at least one month after botulinum toxin application. Clinical assessment involved range of movement measurement, the evaluation of spasticity with Modified Ashworth scale, Global Assesment of Spasticity Scale (GSA), assessment of motor performance with the use of GMFM test and Functional Independence Meassure for children WeeFIM and summarized physical therapeutic methods used in treatment of patients before and after the application. The second group consists of 39 patients who administered the GSA survey forms within a period of one month. The results confirmed positive effect of botulinum toxin treatment on reduction of spasticity, increasing of range of movement and improvement of motor performance of children. Powered by...
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