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Detecting Objects in Images
Kubínek, Jiří ; Beran, Vítězslav (referee) ; Hradiš, Michal (advisor)
This work is dedicated to methods used for object detection in images. There is a summary of several approaches and algorithms to solve this matter, especially AdaBoost algorithm with its improvement, WaldBoost and several features used for object detection. Vital part of this work is dedicated to extending training datasets for classifier training and extending the current object detection framework with histogram of gradients features implementation. Integral part of this work is analysis of results by experiments evaluation.
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Tungsten Trioxide Thin Films Deposited by Material Printing of Aqueous Precursors
Martinková, Eliška ; Pekárková, Jana (referee) ; Králová, Marcela (advisor)
This bachelor thesis deals with material printing of thin layers of tungsten trioxide with selected templating agents. Polyvinyl pyrrolidone, polyoxyethylene(20)cetyl ether, polyethylene glycol and Pluronic 123 were used as templating agents. These compositions were applied by the material printing method on FTO glasses and annealed at 500 °C. The structure and thickness of thin films were analyzed using a profilometer, SEM and AFM. Subsequently, the properties of thin films were studied using photoelectrochemical measurements.
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The roles of an institute of a temporary planning process
Joja, Marie
A strategy of temporary use provides an opportunity for obtaining new ideas in the process of testing different concepts of permanence. It enables municipalities and other authorities to experiment with various approaches to abandoned areas, vacant plots and unused buildings. The proposal of the paper is to define the main roles of an independent institution which serves as a mediator among all the parties involved in the process of temporary use - owners of premises, potential temporary users and the neighbouring community. The research analyses case studies of the most significant temporary planning institutes in Europe and summarises the general principles of a temporary planning institute. Municipalities working with a proposed temporary planning institute possess a number of tools for the implementation of legible procedures of temporary use in the process of activation of neglected areas.
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Utilization of protein radical foootprinting for stuctural biology
Polák, Marek ; Novák, Petr (advisor) ; Junková, Petra (referee)
(In English) The reaction of highly reactive oxygen radicals with protein solvent-accessible residues can be utilized to map protein landscape. Fast photochemical oxidation of proteins (FPOP) is an MS- based technique, which utilizes highly reactive radical species to oxidize proteins and map protein surface or its interactions with their interaction partners. In this work, FPOP was employed to study protein-DNA interactions. First, a full-length of FOXO4-DBD was successfully expressed and purified. The ability of the protein to bind its DNA-response element was verified by electrophoretic and MS-based techniques, respectively. Optimal experimental conditions were achieved to oxidize the protein itself and in the presence of DNA, respectively. Oxidized samples were analyzed by bottom-up and top-down approach. In the bottom-up experiment, modification of individual residues was precisely located and quantified. Different extend of modification was observed for protein alone and in complex with DNA. To avoid experimental artifacts analyzing multiply oxidized protein, standard bottom up approach was replaced by a progressive top-down technology. Only a singly oxidized protein ion was isolated, and further fragmented by collision-induced dissociation (CID) and electron-capture dissociation (ECD),...
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Utilization of protein radical foootprinting for stuctural biology
Polák, Marek ; Novák, Petr (advisor) ; Junková, Petra (referee)
(In English) The reaction of highly reactive oxygen radicals with protein solvent-accessible residues can be utilized to map protein landscape. Fast photochemical oxidation of proteins (FPOP) is an MS- based technique, which utilizes highly reactive radical species to oxidize proteins and map protein surface or its interactions with their interaction partners. In this work, FPOP was employed to study protein-DNA interactions. First, a full-length of FOXO4-DBD was successfully expressed and purified. The ability of the protein to bind its DNA-response element was verified by electrophoretic and MS-based techniques, respectively. Optimal experimental conditions were achieved to oxidize the protein itself and in the presence of DNA, respectively. Oxidized samples were analyzed by bottom-up and top-down approach. In the bottom-up experiment, modification of individual residues was precisely located and quantified. Different extend of modification was observed for protein alone and in complex with DNA. To avoid experimental artifacts analyzing multiply oxidized protein, standard bottom up approach was replaced by a progressive top-down technology. Only a singly oxidized protein ion was isolated, and further fragmented by collision-induced dissociation (CID) and electron-capture dissociation (ECD),...
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Landscape planning study as a tool for physical planning
Masojídková, Petra ; Perlín, Radim (advisor) ; Šantrůčková, Markéta (referee)
The diploma thesis focuses on the new tool Landscape planning study for the municipality with extended competence. The main aim of this work is to find out, how Landscape planning study can be used, who can use it and how it may change the landscape. The thesis uses the case study methods of three selected municipalities with extended competence, namely Votice, Humpolec and Vodňany. The theoretical part discusses the concept of landscape and anchoring in planning activities. In next part, the thesis deals with the basic forms of tools that regulate the planning and using of landscape in Czechia, focusing mainly on spatial planning. Furthermore, the thesis deals with models that currently affect space planning, namely top-down and bottom-up models. Two basic hypotheses of the diploma thesis are set based on theoretical frameworks. There are two qualitative research methods used in the practical part of the thesis. The first method is the analysis of Landscape planning studies, second method are semi-structured interviews with selected respondents. The results of both methods show that Landscape planning study can be used only to a limited extent, mainly as an information source. However, the implementation rate of specific measures is very low now. The identified causes are the lack of...
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The partnership on examples of joint projects of the local action groups of the Pardubice region
Havelková, Iveta ; Pělucha, Martin (advisor) ; Macháček, Jaroslav (referee)
The diploma thesis The partnership on examples of joint projects of the local action groups of the Pardubice region approaches one of the forms of cooperation in which decentralization of the decision-making power is at the level of local actors. The theoretical part focuses on rural areas, local rural development actors, the LEADER method, including Community-Led Local Development, partnerships and local action groups. Rural development groups are made up of representatives of public administration, local entrepreneurs, non-profit organizations and the public. Support is provided by the LEADER method, which works on the bottom-up principle. This method contributes to better targeting of support to rural areas to the well-defined local needs of the population. The practical part focuses on local action groups from the region with a focus on the period of 2007-2013 and the implementation of cooperation projects. The aim of the diploma thesis is to identify the attitudes of local development actors towards cooperation projects, to clarify the relations between groups of local events in the Pardubice Region, to evaluate their elementary cooperation and to identify the bottom-up application rate.
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Initiative EU LEADER as a new approach to the rural development policy and it's contribution to creation of cooperation between different actors of rural development at local level
Pejcharová, Leona ; Perottino, Michel (advisor) ; Svačinová, Tereza (referee)
This diploma thesis deals with a new approach to the rural development in the EU, which is the LEADER initiative. This initiative is significative by involving of local actors to the rural development, elaboration of coherent strategy of development and the bottom-up approach. First part of this thesis deals with the level of exercise of this principle in two concrete member states. I choosed two very different states for my comparaison: Czech republic as a new member state (2004), which has never used LEADER initiative and France as one of six founders of the EC, a country, where the LEADER initiative has been exercised since it's begginings in 1991. I've concluded my analyse of the implementation of this initiative (or sub-measure of LEADER+ type) by finding, that the bottom-up principle is exercited better in France than in the Czech republic. The selection of projects to be financed by LEADER initiative had to be approved directly by the Ministry of Agriculture of the Czech Republic. The selection in France was done only by local action groups. I've realised a comparative case study of two concrete local action groups LAG Posázaví, o.p.s. (Czech Republic, Středočeský kraj) and LAG Pays d'Albret (France, Aquitane region) in the second part of my thesis. My target was to ratify or disprove a...
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LEADER and rural development (the case study of LAG Posázaví)
Onderčaninová, Lucie ; Lošťák, Michal (advisor) ; Boukalová, Kateřina (referee)
Thesis deals with the LEADER method as a specific tool for the rural development. Its aim is to detect how is LAG Posázaví fulfilling principles LEADER in its activities and project implementations.
The empirical part will be based on a content analysis of all completed projects of LAG Posázaví through programs LEADER CZ, LEADER+ a LEADER(SPL) which will evaluate an extent of particular LEADER principles.
The creation of conceptual outsets for the empiric part of the thesis will allow literary research, which puts some theories of rural development and projection into exogenous and endogenous model of rural development. Thesis emphasizes the endogenous rural development and individual principles on which the LEADER approach is based.
The research results demostrate the long-term upward trend of implementing the LEADER principles in projects implemented by LAG Posázaví. While the variable "implementation of innovative approaches" is in the long term constant value
in the last two reporting periods, the other monitored variables are on a steady growth. The upward trend was surprisingly not evident within the short term development.
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Detecting Objects in Images
Kubínek, Jiří ; Beran, Vítězslav (referee) ; Hradiš, Michal (advisor)
This work is dedicated to methods used for object detection in images. There is a summary of several approaches and algorithms to solve this matter, especially AdaBoost algorithm with its improvement, WaldBoost and several features used for object detection. Vital part of this work is dedicated to extending training datasets for classifier training and extending the current object detection framework with histogram of gradients features implementation. Integral part of this work is analysis of results by experiments evaluation.
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