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Determination of antimicrobial activity of berry fruit extracts
Procházková, Lucie ; Veselá, Mária (referee) ; Vítová, Eva (advisor)
The goal of this bachelor´s thesis was the determination of effect of model sample of chokeberry extract (Aronia melanocarpa) on selected types of microorganisms (bacteria Bacillus cereus and Escherichia coli, yeast Candida glabrata). Two commercially available samples of natural extracts from raspberry and cocoa were selected and analysed for comparison. Theoretical part is focused on characteristic, composition and properties of mentioned plant materials, there are also listed their uses in pharmacy, medicine and cosmetics and possible methods of determination of antimicrobiological activity. Analysed commercially available samples of raspberries and cocoa don´t show any antimicrobial activity to selected microorganisms with the exception of 100% raspberry extract on Candida glabrata. There was growth promotion of colonies in the surroundings of the extract in other microorganisms. Ethanol extract of chokeberry fruits showed growing antimicrobial activity with increasing concentration of the extract. The strongest potential was observed in yeast Candida Glabrata and in bacteria Bacillus cereus. The bacteria Escherichia coli was completely resistant.
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Determination of antimicrobial activity of berry fruit extracts
Procházková, Lucie ; Veselá, Mária (referee) ; Vítová, Eva (advisor)
The goal of this bachelor´s thesis was the determination of effect of model sample of chokeberry extract (Aronia melanocarpa) on selected types of microorganisms (bacteria Bacillus cereus and Escherichia coli, yeast Candida glabrata). Two commercially available samples of natural extracts from raspberry and cocoa were selected and analysed for comparison. Theoretical part is focused on characteristic, composition and properties of mentioned plant materials, there are also listed their uses in pharmacy, medicine and cosmetics and possible methods of determination of antimicrobiological activity. Analysed commercially available samples of raspberries and cocoa don´t show any antimicrobial activity to selected microorganisms with the exception of 100% raspberry extract on Candida glabrata. There was growth promotion of colonies in the surroundings of the extract in other microorganisms. Ethanol extract of chokeberry fruits showed growing antimicrobial activity with increasing concentration of the extract. The strongest potential was observed in yeast Candida Glabrata and in bacteria Bacillus cereus. The bacteria Escherichia coli was completely resistant.
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Bioaktivní látky v bobulovém ovoci
Rosová, Iveta
The bachelor thesis on the topic of bioactive substances in berries was processed at the Faculty of Horticulture, Mendel University in the Institute of Post-Harvest Technology of Horticultural Products in 2012 - 2014. The thesis is divided into a theoretical part and a practical part. The theoretical part describes the effect of bioactive substances on human health, various kinds of berries and their health-promoting substances and there is also explained the concept of antioxidant capacity. In the practical part are introduced the varieties of raspberries, red and black currants, that are selected for analysis. This part describes the methods used for analysis as well. Using various methods, it was found that the highest content of total polyphenols and the greatest antioxidant capacity has the frozen fruit, on the contrary fresh fruit was evaluated as the least valuable from the perspective of the mentioned substances.
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