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Evaluation of basic characteristics of thermal water with separation methods
Skálová, Lucie ; Mravcová, Ludmila (referee) ; Vávrová, Milada (advisor)
The presence of inorganic ions is one of the criteria for thermal water assessing. The most important anions that affect the water quality and allow their use for therapeutic purposes, include sulfates, chlorides, nitrates and bicarbonates. Ions (as sodium, potassium, calcium and magnesium) are the significant cations. The ion chromatography is used for separation of these substances and also some methods based on electrophoretic migration of ions in an electric field. The electromigration method of isotachophoresis was chosen for determination of selected ions in water samples collected from thermal boreholes in Pasohlávky.
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Optimization of a method for the determination of inorganic ions using capillary electrophoresis
Špačková, Simona ; Bezděková, Jaroslava (referee) ; Vaculovičová, Markéta (advisor)
Inorganic ions are one of the most important group chemicals. Their importance is crucial for all living organisms on the planet and monitoring of their content is essential for this purpose in clinical or environmental analysis but also in the food industry, agriculture or many other industries. Many analytical methods for ion detection have been designed and used, depending on the area of their use. Recently, numerous methods have been adapted to areas where not primarily intended. One such methods may be, for example, laser ablation with inductively coupled plasma mass spectrometry, which has recently begun to affect the analysis of biological samples. As a complementary method, able to detect also non-metallic ions is capillary electrophoresis. In this master thesis, the possibility of applying capillary electrophoresis with indirect photometric detection for the separation of inorganic ions in cell lysates of HeLa cells by capillary electrophoresis was verified. Main attention was paid to chloride ions. The ability to separate inorganic compounds in cellular material using salicylic acid as an absorbing anion in the base electrolyte was tested.
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Optimization of a method for the determination of inorganic ions using capillary electrophoresis
Špačková, Simona ; Bezděková, Jaroslava (referee) ; Vaculovičová, Markéta (advisor)
Inorganic ions are one of the most important group chemicals. Their importance is crucial for all living organisms on the planet and monitoring of their content is essential for this purpose in clinical or environmental analysis but also in the food industry, agriculture or many other industries. Many analytical methods for ion detection have been designed and used, depending on the area of their use. Recently, numerous methods have been adapted to areas where not primarily intended. One such methods may be, for example, laser ablation with inductively coupled plasma mass spectrometry, which has recently begun to affect the analysis of biological samples. As a complementary method, able to detect also non-metallic ions is capillary electrophoresis. In this master thesis, the possibility of applying capillary electrophoresis with indirect photometric detection for the separation of inorganic ions in cell lysates of HeLa cells by capillary electrophoresis was verified. Main attention was paid to chloride ions. The ability to separate inorganic compounds in cellular material using salicylic acid as an absorbing anion in the base electrolyte was tested.
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Development of electrophoretic method for determination of sulphate in blood plasma
Hnízdilová, Lucie ; Křížek, Tomáš (advisor) ; Hraníček, Jakub (referee)
Cílem této bakalářské práce bylo vyvinout metodu pro stanovení síranů v krevní plazmě pomocí kapilární zónové elektroforézy. Byla použita křemenná kapilára o vnitřním průměru 50 μm a efektivní délce 41,5 cm. Plazmatické proteiny byly sráženy acetonitrilem. Jako optimální základní elektrolyt byla určena 0,4M kyselina mravenčí s 15mM chromanem draselným. Separační napětí bylo 25 kV. Vzorek byl dávkován napětím -5 kV po dobu 40 s. K vyhodnocení byla využita nepřímá UV detekce při 260 nm. Vzhledem ke složité matrici je vhodné provést kvantifikaci metodou standardního přídavku. Po vztažení ploch píků na vnitřní standard (500μM chlorečnany) byla relativní směrodatná odchylka 5,97 %. Výhodou této metody oproti dříve používaným srážecím metodám je krátká doba analýzy a jednodušší úprava vzorku. Klíčová slova kapilární elektroforéza, anorganické ionty, sírany, krevní plazma
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Evaluation of basic characteristics of thermal water with separation methods
Skálová, Lucie ; Mravcová, Ludmila (referee) ; Vávrová, Milada (advisor)
The presence of inorganic ions is one of the criteria for thermal water assessing. The most important anions that affect the water quality and allow their use for therapeutic purposes, include sulfates, chlorides, nitrates and bicarbonates. Ions (as sodium, potassium, calcium and magnesium) are the significant cations. The ion chromatography is used for separation of these substances and also some methods based on electrophoretic migration of ions in an electric field. The electromigration method of isotachophoresis was chosen for determination of selected ions in water samples collected from thermal boreholes in Pasohlávky.
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