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The effect of temperature on plant growth: teaching protocols of model experiments
Máhrlová, Zuzana ; Albrechtová, Jana (advisor) ; Mourek, Jan (referee)
This diploma thesis is focused on the creation of model tasks (by means of an experiment) for the study of biology at secondary schools and concerns the effects of temperature on plant growth. The selected tasks utilize the new didactic approach by means of the so called inquiry based education. This method of teaching contributes to the development of individual deduction reasoning and encourages the students to actively think about the subject matter. The thesis provides the necessary theoretical background for the topic together with the description of methods and material needed for the conducting the experiments. Furthermore, the thesis describes the necessary instructions and procedures to conduct the experiment and its use in the secondary school teaching practice and presents the requisite methodical guidelines and worksheets for them. Simultaneously, the thesis verifies the didactic applicability of the experiment and the worked out materials through a questionnaire survey and monitored application of the model tasks at secondary schools. The main result of this diploma thesis is a modern, functional and verified method of teaching for high school about the effects of temperature on plant growth in accordance with modern approaches to education, which include own experiments, their...
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Response of different genotypes of Zea mays L. and Vicia faba L. treated with brassinosteroids to water deficit
Kuklíková, Radka ; Rothová, Olga (advisor) ; Hnilička, František (referee)
This work monitors the response of plants to the application of a solution of synthetic brassinosteroid (5-fluor-3α,17β-dihydroxy-5α-pregnan-6-on) under drought stress conditions and subsequent restoration of water availability. The response of two plant species was monitored under six-day period of cessation of watering and under six-day period of renewed optimal water supply. The experiments were carried out on plants with different type of photosynthesis - C3 plant Vicia faba L. and C4 plant Zea mays L.. For both plant species, three varieties/genotypes were used. Photosynthetic, morphological and developmental characteristics were monitored. The brassinosteroid treatment influenced more the morphological than the photosynthetic characteristics. The content of photosynthetic pigments was influenced more than the chlorophyll fluorescence parameters. The differences in morphological characteristics between individual varieties/genotypes were observed. The response to the application of brassinosteroid was observed especially for drought-sensitive variety/genotype. Application of brassinosteroid caused increase of the dry mass of leaves and roots, the height to leaf , the height of the whole plant and its increment under stress and post-stress periods in both plant species. The content of...
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The effect of 24-epibrassinolide on selected parameters of maize and faba bean genotypes varying in drought sensitivity
Řehořová, Kateřina ; Kočová, Marie (advisor) ; Hniličková, Helena (referee)
Brassinosteroids are plant hormones which are also known for their pleiotropic effects on plants exposed to various biotic and abiotic stress factors. The aim of this study was to evaluate the role of 10-8 M 24-epibrassinolide in Zea mays L. and Vicia faba L., grown under conditions of drought stress (6, 10, 14, 18 days) with an emphasis on different answer to their drought sensitive and resistant genotypes. Net photosynthetic rate (PN) and transpiration rate (E), stomatal conductance (gs), osmotic potential (ψs), proline (Pro) and malondialdehyde (MDA) content and membrane injury index (MI) were measured in stressed plants and plants grown under controlled conditions. The most important factor influencing these parameters was drought, which led to a gradual decrease of PN, E, gs, ψs and increase of Pro, MDA a MI. The effect of 24-epibrassinolid was significant rather exceptionally and in these cases, more pronounced response was observed in drought stressed plants compared with plants grown under controlled conditions. Plants showed intraspecific variability in their reactions, which in sensitive and tolerant genotypes were not clear and differed depending on the studied parameters and conditions of plant cultivation.
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The effect of temperature on plant growth: teaching protocols of model experiments
Máhrlová, Zuzana ; Albrechtová, Jana (advisor) ; Mourek, Jan (referee)
This diploma thesis is focused on the creation of model tasks (by means of an experiment) for the study of biology at secondary schools and concerns the effects of temperature on plant growth. The selected tasks utilize the new didactic approach by means of the so called inquiry based education. This method of teaching contributes to the development of individual deduction reasoning and encourages the students to actively think about the subject matter. The thesis provides the necessary theoretical background for the topic together with the description of methods and material needed for the conducting the experiments. Furthermore, the thesis describes the necessary instructions and procedures to conduct the experiment and its use in the secondary school teaching practice and presents the requisite methodical guidelines and worksheets for them. Simultaneously, the thesis verifies the didactic applicability of the experiment and the worked out materials through a questionnaire survey and monitored application of the model tasks at secondary schools. The main result of this diploma thesis is a modern, functional and verified method of teaching for high school about the effects of temperature on plant growth in accordance with modern approaches to education, which include own experiments, their...
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Response of different genotypes of Zea mays L. and Vicia faba L. treated with brassinosteroids to water deficit
Kuklíková, Radka ; Rothová, Olga (advisor) ; Hnilička, František (referee)
This work monitors the response of plants to the application of a solution of synthetic brassinosteroid (5-fluor-3α,17β-dihydroxy-5α-pregnan-6-on) under drought stress conditions and subsequent restoration of water availability. The response of two plant species was monitored under six-day period of cessation of watering and under six-day period of renewed optimal water supply. The experiments were carried out on plants with different type of photosynthesis - C3 plant Vicia faba L. and C4 plant Zea mays L.. For both plant species, three varieties/genotypes were used. Photosynthetic, morphological and developmental characteristics were monitored. The brassinosteroid treatment influenced more the morphological than the photosynthetic characteristics. The content of photosynthetic pigments was influenced more than the chlorophyll fluorescence parameters. The differences in morphological characteristics between individual varieties/genotypes were observed. The response to the application of brassinosteroid was observed especially for drought-sensitive variety/genotype. Application of brassinosteroid caused increase of the dry mass of leaves and roots, the height to leaf , the height of the whole plant and its increment under stress and post-stress periods in both plant species. The content of...
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Inter- and intraspecific variability in Vicia faba L. and Zea mays L. response to water deficit
Fridrichová, Lenka ; Holá, Dana (advisor) ; Zelenková, Sylva (referee)
This work monitors the response of plants to water deficit and subsequent restoration of water availability with the aim to expand our knowledge about plant behaviour during and after drought stress from the perspective of two plant species with a different type of photosynthesis. A particular attention was paid to the intraspecific differences and their possible changes during stress and post-stress periods. The plant response was monitored as the changes of selected photosynthetic, morphological and developmental characteristics caused by the cessation of watering for six days followed by a six-day period of renewed optimal water supply. The experiments were carried out on plants with different types of photosynthesis - C3 plant Vicia faba L and C4 plant Zea mays L.; for both plant species, three varieties / genotypes were monitored. The response of both plant species to insufficient water supply differed by the extent of changes in relative water content (RWC), chlorophyll fluorescence parameters, the dry mass of individual leaves (developed during the stress period), the ratio between the aboveground dry mass and dry mass of roots. In drought-stressed plants, downward trend in RWC was observed, as well as the increase of the minimum yield of chlorophyll fluorescence in dark-adapted leaves (F ),...
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