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National Repository of Grey Literature

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Connection between the solubility and the mutagenicity of nitrobenzanthrones in water and organic solvents Řeboun, Martin ; Martínek, Václav (advisor) ; Hudeček, Jiří (referee) 2-Nitrobenzanthron (2-NBA) and 3-nitrobenzanthron (3-NBA) are pollutants widely occurring in the environment. The main sources of benzanthrones are combustion products (i.e. diesel exhausts, wood and cigarette smoke ...). 3-NBA is proven mutagen and carcinogen for bacteria and mammals and it is probably mutagenic also to humans. Nitronbezanthrones can form covalent adducts with purine bases in DNA after their metabolic activation. Genotoxic properties of 2-NBA are lover by 3-4 orders of magnitudes in comparison to 2-NBA, large difference in the solubility, and therefore also the difference in their bioavailability, of these isomers can be among the factors explaining this phenomenon. One of our goals was to determine the solubility of 3-NBA and 2-NBA in water and in methanol. The second objective was to determine the extinction coefficients for their solutions in water and methanol. Two different methods were employed to determine the solubility of 3-NBA and 2-NBA. The first method was based on stepwise adding of the stock solution of 3-/2-NBA in dimethylsulfoxide to excessive volume of water and simultaneous spectrophotometric verification of the Lambert-Beer law. Additional experiments confirmed the solubility of 3-/2- NBA utilizing spectrophotometric determination of 3-/2-NBA concentration in... Detailed record

Connection between the solubility and the mutagenicity of nitrobenzanthrones in water and organic solvents Řeboun, Martin ; Martínek, Václav (advisor) ; Hudeček, Jiří (referee) 2-Nitrobenzanthron (2-NBA) and 3-nitrobenzanthron (3-NBA) are pollutants widely occurring in the environment. The main sources of benzanthrones are combustion products (i.e. diesel exhausts, wood and cigarette smoke ...). 3-NBA is proven mutagen and carcinogen for bacteria and mammals and it is probably mutagenic also to humans. Nitronbezanthrones can form covalent adducts with purine bases in DNA after their metabolic activation. Genotoxic properties of 2-NBA are lover by 3-4 orders of magnitudes in comparison to 2-NBA, large difference in the solubility, and therefore also the difference in their bioavailability, of these isomers can be among the factors explaining this phenomenon. One of our goals was to determine the solubility of 3-NBA and 2-NBA in water and in methanol. The second objective was to determine the extinction coefficients for their solutions in water and methanol. Two different methods were employed to determine the solubility of 3-NBA and 2-NBA. The first method was based on stepwise adding of the stock solution of 3-/2-NBA in dimethylsulfoxide to excessive volume of water and simultaneous spectrophotometric verification of the Lambert-Beer law. Additional experiments confirmed the solubility of 3-/2- NBA utilizing spectrophotometric determination of 3-/2-NBA concentration in... Detailed record

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