National Repository of Grey Literature 4 records found  Search took 0.01 seconds. 
Fluorescence of organic dye in free form or bound on metal nanoparticles analysis using confocal microscopy
Mocko, Štefan ; Chmelíková, Larisa (referee) ; Čmiel, Vratislav (advisor)
This bachelor`s thesis deals with the long-term analysis of the fluorescence of rhodamine dye, which is linked to the ferric SPIO nanoparticles. The first part introduces the necessary scientific basis for understanding the physical phenomenon of fluorescence. It also focuses on the hardware and software for the development of long-term analysis of fluorescence for mesenchymal stem cells.Finally, there is described an analytical software to work with measured data, which was developed for this work.
Software FLIM system in confocal microscopy
Petrula, Jakub ; Odstrčilík, Jan (referee) ; Čmiel, Vratislav (advisor)
This bachelor thesis deals with the FLIM system utilization in confocal microscopy. It describes basic concepts which are closely related to the topic, such as principles of fluorescence, fluorescent dyes and principles of both fluorescence and confocal microscopy. Practical part involves work with the confocal microscope and set of intensity images acquisition of both the animal and plant cells. Final product of pratical part is fluorescence lifetime image. The thesis also describes an algorithm for generating a pseudocolor frames and created user interface.
Fluorescence of organic dye in free form or bound on metal nanoparticles analysis using confocal microscopy
Mocko, Štefan ; Chmelíková, Larisa (referee) ; Čmiel, Vratislav (advisor)
This bachelor`s thesis deals with the long-term analysis of the fluorescence of rhodamine dye, which is linked to the ferric SPIO nanoparticles. The first part introduces the necessary scientific basis for understanding the physical phenomenon of fluorescence. It also focuses on the hardware and software for the development of long-term analysis of fluorescence for mesenchymal stem cells.Finally, there is described an analytical software to work with measured data, which was developed for this work.
Software FLIM system in confocal microscopy
Petrula, Jakub ; Odstrčilík, Jan (referee) ; Čmiel, Vratislav (advisor)
This bachelor thesis deals with the FLIM system utilization in confocal microscopy. It describes basic concepts which are closely related to the topic, such as principles of fluorescence, fluorescent dyes and principles of both fluorescence and confocal microscopy. Practical part involves work with the confocal microscope and set of intensity images acquisition of both the animal and plant cells. Final product of pratical part is fluorescence lifetime image. The thesis also describes an algorithm for generating a pseudocolor frames and created user interface.

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