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National Repository of Grey Literature 2 records found  Search took 0.00 seconds. 
The significance of extracellular DNA in osteoclastogenesis from peripheral blood precursors - in vitro study
 Jelínková, Ivana ; Daňková, Pavlína (advisor) ; Korabečná, Marie (referee)
Introduction: Extracellular DNA (ecDNA) is a common component of blood plasma. Increased levels of ecDNA in plasma can be found in some autoimmune diseases like systemic lupus erythematosus (SLE), rheumatoid arthritis or celiac disease which are associated with inflammatory processes. These diseases are also associated with an increased risk of osteoporosis. Bone is a dynamic structure undergoing constant modelling caused by osteoblasts, osteocytes and osteoclasts. Shifting their equilibrium can lead to pathological conditions such as osteoporosis. In this thesis we focused on elucidating whether ecDNA, an inflammatory agent with proven immunoregulatory effects can alter differentiation potential of monocytes and alternatively lead to osteoclastogenesis via TLR9. Material and methods: We obtained monocytes from peripheral blood of healthy donors and cultivated them with four types of ODNs control (CO), stimulatory (ST), inhibitory (INH, telomeric (TLM) with phosphodiester (-pO) or phosphorothioate (-pS) backbone for two weeks to establish their effect on differentiation potential of monocytes into osteoclasts. Osteoclastogenesis was evaluated by number of yielded osteoclasts observed on a light microscope. To establish the effect of ODNs on osteoclast activity samples were analysed by qPCR for...
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Interactions of mouse polyomavirus with Toll-like receptors
 Pokorná, Karolína ; Forstová, Jitka (advisor) ; Němečková, Šárka (referee)
Toll-like receptors (TLRs) are important receptor family of innate immunity. They enable fast recognition of infection through so called pathogen associated molecular patterns (PAMPs). In this thesis, we studied interaction of mouse polyomavirus (MPyV) with TLRs of mouse embryonic fibroblasts (MEF cells). We observed that inhibition of TLR4 signaling abolished response of MEF cells to MPyV. This suggested that TLR4 plays a role in MEF cells recognition of MPyV. To detect response of MEF cell to MPyV, we measured IL-6 production by ELISA. Next, we investigated effect of TLR4 signalization on MPyV infection. Inhibition of TLR4 signaling with CLI-095 inhibitor did not affect number of infected cells. Presence of TLR4 antagonist, LPS-RS, led to significant decrease in quantity of infected cells 20 hours post infection. Decrease in number of infected cells was also observed in presence of LPS. Viral infection was also inhibited by TLR9 antagonist ODN 2088. We also investigated role of MAP kinases in MPyV infection. We tested, whether inhibition of selected MAP kinases would affect number of infected cells. Inhibition of kinase p38 did not affect infection. On the other hand, inhibition of MEK kinase or JNK resulted in decrease of number of cells infected by MPyV.
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