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Regulation of potato tuber onset: the role of auxin
Myslivcová, Jitka ; Mašková, Petra (advisor) ; Havelková, Lenka (referee)
Potato (Solanum tuberosum) is an essential component of food worldwide. The process of tuber formation is controlled by the interplay of external and internal conditions. Understanding of the molecular basis of tuberization, including the effect of photoperiod, pointed to a key role of mobile signals. Among these morphogenic signals StBEL5 and POTH1 transcripts are involved, which are translated in the stolon and form a heterodimer regulating target genes with TTGAC tandem motifs, further an FT homologue, StSP6A, mobile from leaves to stolons as a protein. BEL5/POTH1 and StP6A signaling pathways seem to overlap. Phytohormones also play an important signaling role, i.a. auxins. During tuber initiation, IAA levels in stolon tissues increase. PIN transporters are likely to be involved in the redistribution of auxin in the stolon, but also LAX and ABCB transporters and components of auxin signaling pathway, Aux/IAAs and ARFs are also involved in the process. Although auxins are proved to play an important role during stolon-to-tuber transition, their exact role in the process is not sufficiently clarified. Auxin signaling is interconnected with BEL5/POTH1 and SP6A-mediated tuber signaling, as its target genes in the stolon include those that encode proteins involved in auxin biosynthesis (YUCCA1),...
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Modulation of BELL transcription factors balance as a tool for tuberisation strengthening in selected potato cultivars
Zounková, Andrea ; Mašková, Petra (advisor) ; Fischer, Lukáš (referee)
Potato (Solanum tuberosum) is the third most important crop in terms of human consumption. Hence understanding regulatory mechanisms controlling tuber initiation is significant not only from a theoretical but also practical point of view. Tuberization of S. tuberosum is a complex morphogenic process regulated by both, environmental conditions and inner plant status. Environmental conditions regulating the onset of tuberization have been known for a long time, but the molecular basis of tuber initiation has only recently been discovered, in particular using wild subspecies S. tuberosum subsp. andigena. BELL transcription factors are among the important components of the regulatory signalling network. The aim of this study was to modulate balance between the BELL transcription factors that induce and repress tuberization towards strengthening of tuber induction using two cultivars of Solanum tuberosum subsp. tuberosum, cv. Kamýk, resp. cv. Korela. To achieve this, transgenic lines carrying construct containing a part of coding sequence StBEL11, resp. StBEL29 in antisense orientation (AS) under control of constitutive promoters and lines carrying complete sequences of StBEL5 and StPTB6 genes under control of their native promoters were derived. We used the StBEL11 and StBEL29 AS constructs with the aim to...
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Modulation of BELL transcription factors balance as a tool for tuberisation strengthening in selected potato cultivars
Zounková, Andrea ; Mašková, Petra (advisor) ; Fischer, Lukáš (referee)
Potato (Solanum tuberosum) is the third most important crop in terms of human consumption. Hence understanding regulatory mechanisms controlling tuber initiation is significant not only from a theoretical but also practical point of view. Tuberization of S. tuberosum is a complex morphogenic process regulated by both, environmental conditions and inner plant status. Environmental conditions regulating the onset of tuberization have been known for a long time, but the molecular basis of tuber initiation has only recently been discovered, in particular using wild subspecies S. tuberosum subsp. andigena. BELL transcription factors are among the important components of the regulatory signalling network. The aim of this study was to modulate balance between the BELL transcription factors that induce and repress tuberization towards strengthening of tuber induction using two cultivars of Solanum tuberosum subsp. tuberosum, cv. Kamýk, resp. cv. Korela. To achieve this, transgenic lines carrying construct containing a part of coding sequence StBEL11, resp. StBEL29 in antisense orientation (AS) under control of constitutive promoters and lines carrying complete sequences of StBEL5 and StPTB6 genes under control of their native promoters were derived. We used the StBEL11 and StBEL29 AS constructs with the aim to...
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