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Proposals of Practical Excercises in Zoology Based on Protists and Invertebrates from Freshwater Aquaria
Talavášková, Barbora ; Mourek, Jan (advisor) ; Říhová, Dagmar (referee)
This thesis deals with proposals for practical exercises using freshwater aquaria in teaching elementary schools and junior high schools that focus on protozoa and invertebrates. The main aim of the thesis is to propose practical exercises using elements of inquiry-based science education. Using identification cards of protozoa and microscopic invertebrates, pupils can learn about these organisms during laboratory exercises with microscopes. A total of fourteen cards with representatives of protozoa, invertebrates, algae and cyanobacteria typical of freshwater aquariums are presented. Each of the identification cards contains a basic description, as well as a procedure for the pupils to observe and draw the organism. They are accompanied by a photograph, a pen drawing and a QR code that links to a video of the observation of the representative under the microscope. Another proposal is a practical exercise focusing on the food chain of standing water. Also included is a worksheet focusing on independent laboratory work by students on water quality analysis using the Pasco laboratory technique. The contribution of this work is the actual experience of setting up a school freshwater aquarium. The process of setting up the aquarium and investigating it is described in this thesis. The succession of...
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Střevní paraziti ptáků na Svalbardu
ŽÁNOVÁ, Veronika
The study aimed to obtain essential knowledge about the distribution of intestinal parasites of birds occurring in Svalbard. Noninvasive coprological techniques were used to detect coccidian, cryptosporidian and microspordian parasites. The research included field collection of faecal samples and laboratory examination by microscopic and molecular methods.
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Methodology of detection of tick-borne pathogens and statistics of their occurrence in the Czech Republic
BROŽKOVÁ, Zdeňka
This bachelor thesis deals with tick-borne transmitted pathogens and with ticks alone. In general, it focuses on ticks species, that are able to infect people. The Thesis is divided into three parts. The goal of the theoretical part is to refer that borreliosis and tick encephalitis are not unique infection diseases endangering human health. In this thesis, infection diseases are divided into bacterial, viral and protozoan. The core of my work is the empirical part, that uses the method of tick-borne pathogen diagnosis. In the practical part I have tried to detect pathogens in ticks by means of PCR and RT-PCR. The third part is based on statistical indexes, where I have compared laboratory results from years 2008-2018. I have analysed the results for a period of 10 years of testing and simultaneously the results of various infection diseases. Statistical data were compared with reports of infection diseases occurrence from the National Institute of Public Health in the time period.
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Parasites in nutria (Myocastor coypus)
Urbanová, Beáta ; Langrová, Iva (advisor) ; Nechybová, Stanislava (referee)
The aim of this work was published data concerning parasites coypu (Myocastor coypus).
The first part describes the anatomy and the ethology of a coypu. Nutria is a relatively large rodent, native from South America. It lives near the water areas in small family groups which are lead by the old female. Its body is adapted to the life in water. Nutria is herbivores, which eats littoral and aquatic plants. It is a polyestric animal which gives birth to about six nidifugous pups. The nutria is the non-native species in the Czech Republic. The first import of nutrias was held in 1924 from Argentina. After that they escaped from the farms and due to they got into the wild.
The second part of this work characterizes parasites that have been diagnosed in nutrias. Protozoa diseases cause the coccidia and Toxoplasma. The coccidia are parasites of the genus Eimeria and belong to the most dangerous diseases for nutrias. The trematodes (Fasciola hepatica) also occur in nutrias and causing obturation and destruction of the liver. Anoplocephala sp., Hymenolepis octocornata and Rodentolepis avetjanae from tapeworms parasitize in nutria. Additionally nutrias serve as intermediate hosts of Echinococcus granulosus, Multiceps serialis and Taenia tenuicollis. The diseases, caused by parasitic nematodes from class Nematodes, are caused by parasites Trichinella spiralis, Trichuris myocastoris, the worms of genus Strongyloides (Strongyloides myopotami), and the worms from superfamily Trichostrongyloidae (Trichostrongylus retortaeformis, T. myocastoris). Among the most ectoparasitic diseases frequently belong the scabies (Myocoptes myocastori, Chirodiscoides caviae) and Demodex spp.
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Vliv probiotických krmných aditiv na funkční stav bachoru
HADAČOVÁ, Veronika
In my study I was examining the influence of the probiotic Bifidobacterium sp. on the functional status of the cattle´s rumen. Two adult cows Aberdeen-angus were used in this experiment. They were treated with a permanent cannula, which served for daily dosing of probiotics Bifidobacterium sp.. Samples of rumen fluid were analyzed for the amount of volatile fatty acids, protozoans, pH and the quantity of ammonia. When we tested the effect of the probiotics on each variable, the fixed effect of the influence of an individual has not been proved. When we tested the data without the effect of the individual in a linear model, the variables best describing my data were the butyric and acetic acids. The amount of protozoans increased as there levels grew. There is a strong effect of the individual as only two individuals were used. My results indicate that the influence of the probiotics Bifidobacterium sp., on the functional status of the rumen is low. These results could be affected by the low number of experiment-replication as well as by small quantity of tested animals.
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Endoparasite infections in sheep and goats in diverse farming systems
UHLÍŘOVÁ, Barbora Isatou
Samples of excrements for parasitological investigation were obtained in two year period 2011 and 2012 on three sheep farms and two goat farms in South Bohemia (a farm conventional, an organic and converting to an organic farming system). A total of 400 samples were examined using four parasitological methods. Thirteen species of gastrointestinal nematodes and one species of Protozoa were recovered. In farms Coccidia Eimeria spp. was the most diagnosed. Results revealed that goats were 1,8 times more often infected with parasites than sheep (x2 = 6,274; d. f. = 1; p = 0, 00576; OR = 1,82). Animals younger 6 months were 3 times more often infected with Eimeria than older ones (x2 = 17,174; d. f. = 1; p = 0,00003; OR = 3,16). Goats were 11 times more often infected with parasites in winter than sheep (x2 = 5,8174; d. f. = 1; p = 0,0050; OR = 10,78). Goats were 2 times more often infected with lungworm infection than sheep (x2 = 8,407; d. f. = 1; p = 0,0019; OR = 1,99). A statistically significant difference in infectious contamination between conventional and an organic system bred animals was not discovered (x2 = 0,0145; d. f. = 1; p = 0,452; OR = 1,05).
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