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DNA isolation from selected vegetable products (paprika)
Gőghová, Sabína ; Kuderová,, Alena (referee) ; Kovařík, Aleš (advisor)
The diploma thesis deals with micromethod of DNA isolation from ten differently processing food products containing pepper (Capsicum annum). PCR ready DNA was isolated by magnetic particles PGMA functionalized by carboxyl groups from homogenates prepared in lysis buffer with CTAB. Quantity and quality of DNA was estimated using spectrophotometric measurements and verified using PCR methods with primers specific for plant rDNA. Quality of isolated DNA varied depending on processing technology. DNA isolated from smoked grinded peppers and from heat treated food products was degraded and amplified with primers F_26S and R_26S (PCR product 220 bp) in contrary to the primers F_18S and R_5.8S (PCR product 700 bp). DNA isolated from the other food products was amplified with primers F_18S and R_5.8S (PCR product 700 bp). PCR product from one grinded pepper (Žitavská paprika) was cloned and sequenced.
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Determination of the nutritional properties of paprika of different geographic origin
Kovaříková, Tereza ; Vespalcová, Milena (referee) ; Diviš, Pavel (advisor)
This master thesis deals with the analysis of nutritional properties of paprika condiment with different geographic origin. The theoretical part contains botanical description of red pepper (Capsicum annuum L.), its chemical composition, process of production of paprika and then they are described analytical methods suitable for determination of fundamental nutrional properties. Experimental part is focused on the preparation of samples and their analysis. In total, 11 samples were selected for the experiment, 5 of them had a protected designation of origin. These samples were from Slovakia, Hungary, Bulgary, Romania, Turkey and Spain. The Soxhlet extraction was used for the analysis of the fat content, Kjeldahl method was used to determine gross protein. High-performance liquid chromatography (HPLC) was chosen to determine carbohydrates and capsaicin. Induction-coupled plasma emission spectroscopy (ICP-OES) was used for elemental analysis. The results were compared with each other and with available databases and literature. For comparing and finding certain connections, the principal component analysis (PCA) was selected.
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Determination of basic analytical parameters of paprika of different geographic origin
Cagáňová, Linda ; Vespalcová, Milena (referee) ; Diviš, Pavel (advisor)
The aim of this bachelor thesis was to determine the basic analytical parameters of paprika of different geographic origin such as ASTA colour, pH, moisture content and ash. The theoretical part primarily focuses on the characteristic of paprika, it´s division, the chemical composition and the production of ground red pepper. It also deals with spice issues, it´s division, meaning in and storage. In the experimental part there are outlined the individual procedures of determination of parameters (ASTA, pH, moisture, ash) of ten samples of ground red pepper. ASTA values ranged from 80 – 175 ASTA units. Analysis of pH showed that all samples of pepper extracts showed an acidic pH with value around 5. The total moisture and ash content in the samples was 8 – 15 % and 5 – 7 %.
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Koření rodu Capsicum
Falešník, Adam
The bachelor's thesis deals with the topic of the Capsicum genus spices, mainly chili in the food industry. The thesis includes a literature review on spices, their composition, production, and function. It also discusses the origin, history, characteristics, species, and varieties of the Capsicum genus. The thesis describes their cultivation, processing, chemical composition, and the evaluation of their pungency. Furthermore, the thesis focuses on the effects of the Capsicum genus on human health related to nutrition and summarizes the findings on new trends in the use of chili and its main component, capsaicin, in the food industry.
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Posouzení vlivu země původu a způsobu zpracování na mikrobiotu vybraného koření
Menoušková, Karolína
The diploma thesis deals with the requirements for the quality of spices. It mainly deals with the microbial contamination of spices, factors of influence and possible means to prevent its contamination. Furthermore, the work deals with the characteristics of spices of the genus Capsicum, its range and possible use. In the practical part of the work, the microbial contamination of spices in peppers and chilli was determined. Individual spice samples differed depending on the country of origin, the method of technological processing, physical treatment and the content of capsaicin. During the microbiological analysis, the total number of microorganisms (CPM), the numbers of coliform bacteria and Escherichia coli, moulds and yeast, or the presence of Clostridum perfringens were monitored. The highest CPM value (4,49 log KTJ·g−1) was detected in the sample of ground chilli from China. A higher number of coliform bacteria and E. coli (2,49 log KTJ·g−1) was observed in the sample of sweet pepper from Spain. A significantly higher number of moulds and yeasts (3,13 log KTJ·g−1) was recorded in the sample of ground goulash pepper from the Czech Republic. In the case of Cl. perfringens, the presence of this bacterium was showed in 3 of 13 pepper and chilli spice samples. During the laboratory experiment, no significant deviations of occurrence of the monitored microorganisms were detected in comparison with the recommended limits or the results of scientific studies. The results show that microbial contamination may be affected by the country of origin, the physical treatment, or the alkaloid content of capsaicin, which evidences an antimicrobial activity. When monitoring the effect of technological processing on the spice microbiota, there was no statistically significant difference between the monitored pepper samples (P < 0,05).
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Optimalizace molekulárně-biologických metod pro detekci kontaminant v koření
Došková, Markéta
Contaminants are described in food generally, with narrower aim to spices in literary part of thesis. Bigger attention is dedicated to mycotoxins: secundary products of molds, basic survey, ways of assessment and legislation connected with their monito-ring. The main part of literary searches is focused on methods for microbial quality as-sessment of foods, then on description of classic and modern methods. Whereas samples of dried capsicum and pepper powders served as a samples for practical part, the end of theoretical part is devoted to individual steps for optimalization of new moleculary-biological method. The outputs of each steps are then parts of results and discussion, as well as other prospects and possible ways to future.
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Vliv pomocného přípravku Pentakeep na vybranou modelovou kulturu zeleniny
Želísková, Renata
The object of this thesis was to evaluate the effect of selected auxiliary product Pentakeep Super on Capsicum annuum. The theoretical part approaches the issue of stress factors in plants and the use of Pentakeep Super. The practical part of this thesis described the experiment based on the grounds of the Faculty of Horticulture in 2016. Varieties of Capsicum annuum 'Zlata' and 'Granova' were used as model cultures. The effect of the selected product for improvement of quality and increase of production was studied. In addition the data obtained from the experiment were statistically analyzed. Statistical analysis showed effects on the contentual and morphological parameters of the peppers. Results shows, that the application of Pentakeep have positive effect on mineral elements in fruits.
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Produkce a využití chilli pariky
Frišaufová, Barbora
The aim of this thesis was to compile the available literature sources about origin of chilli peppers, their description and use. Furthermore to focus on the content of chilli peppers and their evaluation, process statistical data of chilli production and interpret them properly. Chilli peppers come from America and were brought to Europe by Christopher Columbus. They then spread throughout the world. Chilli peppers contain a large amount of vitamin C and capsaicin, which has positive effect on human health. This is the main reason why chilli is used in cuisines and medicine troughout the world. Global production of chilli peppers increases every year. The largest producers are mostly Asian countries, especially India.
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DNA isolation from selected vegetable products (paprika)
Gőghová, Sabína ; Kuderová,, Alena (referee) ; Kovařík, Aleš (advisor)
The diploma thesis deals with micromethod of DNA isolation from ten differently processing food products containing pepper (Capsicum annum). PCR ready DNA was isolated by magnetic particles PGMA functionalized by carboxyl groups from homogenates prepared in lysis buffer with CTAB. Quantity and quality of DNA was estimated using spectrophotometric measurements and verified using PCR methods with primers specific for plant rDNA. Quality of isolated DNA varied depending on processing technology. DNA isolated from smoked grinded peppers and from heat treated food products was degraded and amplified with primers F_26S and R_26S (PCR product 220 bp) in contrary to the primers F_18S and R_5.8S (PCR product 700 bp). DNA isolated from the other food products was amplified with primers F_18S and R_5.8S (PCR product 700 bp). PCR product from one grinded pepper (Žitavská paprika) was cloned and sequenced.
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Determination of basic analytical parameters of paprika of different geographic origin
Cagáňová, Linda ; Vespalcová, Milena (referee) ; Diviš, Pavel (advisor)
The aim of this bachelor thesis was to determine the basic analytical parameters of paprika of different geographic origin such as ASTA colour, pH, moisture content and ash. The theoretical part primarily focuses on the characteristic of paprika, it´s division, the chemical composition and the production of ground red pepper. It also deals with spice issues, it´s division, meaning in and storage. In the experimental part there are outlined the individual procedures of determination of parameters (ASTA, pH, moisture, ash) of ten samples of ground red pepper. ASTA values ranged from 80 – 175 ASTA units. Analysis of pH showed that all samples of pepper extracts showed an acidic pH with value around 5. The total moisture and ash content in the samples was 8 – 15 % and 5 – 7 %.
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