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The Role of Bmp4 and Bmp8b proteins in genesis of mouse primordial germ cells
Vegrichtová, Markéta ; Tlapáková, Tereza (advisor) ; Petelák, Aleš (referee)
Primordial germ cells are precursors of sexual breeding organisms. For induction of their formation are necessary growth factors from extraembryonic ectoderm. There are key proteins BMP4 a BMP8b among these factors. These proteins form both homodimers and heterodimers and through receptor complexes and SMAD proteins are indicating the competent cells of proximal epiblast, which give rise to the primordial germ cells and other structures of embryonic mezoderm. Although BMP4 and BMP8b proteins signal similarly, they are mutually indispensable and though for induction of primordial germ cells are both necessary. Up to now, it was proposed several models of their interaction, but not yet completely accurate.
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The Role of Bmp4 and Bmp8b proteins in genesis of mouse primordial germ cells
Vegrichtová, Markéta ; Tlapáková, Tereza (advisor) ; Petelák, Aleš (referee)
Primordial germ cells are precursors of sexual breeding organisms. For induction of their formation are necessary growth factors from extraembryonic ectoderm. There are key proteins BMP4 a BMP8b among these factors. These proteins form both homodimers and heterodimers and through receptor complexes and SMAD proteins are indicating the competent cells of proximal epiblast, which give rise to the primordial germ cells and other structures of embryonic mezoderm. Although BMP4 and BMP8b proteins signal similarly, they are mutually indispensable and though for induction of primordial germ cells are both necessary. Up to now, it was proposed several models of their interaction, but not yet completely accurate.
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Identification and isolation of primordial gonocytes in sturgeon
DVOŘÁK, Matěj
Primordial gonocytes (PGCs) in some animals, including fish arise after fertilization in extragonadal region from maternally inherited germline cytoplasm, and migrate to the future gonads region during embryogenesis, where differentiate into gametes. PGCs formation and migration patterns have been studied in several species models, and it is known that these patterns differ from each other. Sturgeons belong to class ray-finned fishes(Actinoptergii), in which the sturgeon phylogenetic position is an out-group to teleost fishes, the sturgeon development pattern is more similar to amphibians than teleost fishes. In this study, we demonstrate an injection technique for sturgeon PGCs visualization by GFP nos1 3'UTR mRNA. We found that the Sterlet(A. ruthenus) PGCs are specified in the vegetative pole of the embryo. Subsequently, we reported the PGCs migration route. The arisen PGCs actively migrated on the yolky cell mass, yolky extension, and after that passively moved to gonadal ridge. This study provides evidence that the PGCsare specified by maternally inherited germplasm, located in the vegetative part of the embryo. Sturgeon PGCs specification was similar to that of anuras, but the migration pattern resembled that of teleost. Furthermore, we successfully isolated PGCs to next needed studies.
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