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Phospholipid metabolism in the formation of structured yeast colonies
Pavlíčková, Martina ; Schierová, Michaela (advisor) ; Heidingsfeld, Olga (referee)
Yeasts in their natural environment form structured colonies. This allows them to better adapt to environmental conditions, but also to more easily resist various types of yeast infection inhibitors. The metabolism of phospholipids is closely related to the morphology of colonies. An important gene involved in phospholipid metabolism is INO1, which encodes inositol-3- phosphate synthase. Expression of the INO1 gene is regulated by the Opi1p negative transcription factor, which also affects a number of other genes for phospholipid metabolism enzymes, is also necessary for the expression of the FLO11 gene, encoding Flo11p, which is essential to the formation of a structured colony. The main aim of my work was to investigate the correlation between colony morphology of a natural strain of Saccharomyces cerevisiae and phospholipid metabolism. I have found that changes in INO1 gene expression and colony morphology are influenced by carbon source, selenate activity and the inhibitor of β-oxidation, 2-bromooctanoic acid. Although the INO1 gene is not essential for cell viability, its deletion or overexpression causes changes in phospholipid metabolism and colony morphology. Selenate and 2-bromooctanoic acid also alter expression of the FLO11 gene, which is reflected in colony structure. Thus, 2-...
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Phospholipid metabolism in the formation of structured yeast colonies
Pavlíčková, Martina ; Schierová, Michaela (advisor) ; Heidingsfeld, Olga (referee)
Yeasts in their natural environment form structured colonies. This allows them to better adapt to environmental conditions, but also to more easily resist various types of yeast infection inhibitors. The metabolism of phospholipids is closely related to the morphology of colonies. An important gene involved in phospholipid metabolism is INO1, which encodes inositol-3- phosphate synthase. Expression of the INO1 gene is regulated by the Opi1p negative transcription factor, which also affects a number of other genes for phospholipid metabolism enzymes, is also necessary for the expression of the FLO11 gene, encoding Flo11p, which is essential to the formation of a structured colony. The main aim of my work was to investigate the correlation between colony morphology of a natural strain of Saccharomyces cerevisiae and phospholipid metabolism. I have found that changes in INO1 gene expression and colony morphology are influenced by carbon source, selenate activity and the inhibitor of β-oxidation, 2-bromooctanoic acid. Although the INO1 gene is not essential for cell viability, its deletion or overexpression causes changes in phospholipid metabolism and colony morphology. Selenate and 2-bromooctanoic acid also alter expression of the FLO11 gene, which is reflected in colony structure. Thus, 2-...
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Pleiotropic effects of transcriptional protein Opi1 in Saccharomyces cerevisiae
Pavlíčková, Martina ; Schierová, Michaela (advisor) ; Mašek, Tomáš (referee)
Phospholipid biosynthesis in Sacchromyces cerevisiae is regulated by Ino2p-Ino4p activation complex and Opi1p repressor. The most highly regulated INO1 gene encodes inositol-3- phosphate synthase. This enzyme catalyzes the first step of the metabolic pathway of inositol synthesis. The Ino2p-Ino4p activation complex binds to the promoter of the target genes and interacts with other proteins necessary for activation (Snf1p, SAGA, SWI/SNF, INO80). The Opi1p represses transcription by direct binding to Ino2p and by interaction with other proteins (Sin3p, Cyc8p). The activity of Opi1p protein is mediated by cellular localization and by phosphorylation. The regulation of phospholipids is dependent on the growth phase and on the availability of precursors. Apart from its repressor activity, Opi1p affects mitochondrial metabolism, endoplasmic reticulum stress, cell size, mat formation and invasive growth.
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