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Study of the effect of myocardial fibrosis on elemental distribution in rat soft tissues using Laser Ablation ICP-MS
Machalová, Martina ; Zlámalová Gargošová, Helena (referee) ; Vašinová Galiová, Michaela (advisor)
The most common cause of death in the Czech Republic continue to be cardiovascular diseases. The field of heart failure therapy still keeps moving forward. Yet myocardial fibrosis is one of the consequences and causes of heart failure for which there exists no effective medical care. The solution would be predicting the advancement of fibrosis and a preventive therapy. Many medical methods are being developed now in this regard, and the element distribution in affected tissue could help them. That is the reason why this work centers on the research of the element distribution in rat hearts with an induced myocardial fibrosis. An influence of sample couloration on the analysis was observed during its course. Special attention was paid to the distribution of iron in the fibrotic tissue as well as to the comparisson of the images of myocardial fibrosis. It seems that the iron distribution specificly could be helpful in recognising a damaged myocardium.
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Segmentation of Cardiac MR Images
Dohnalová, Petra ; Mézl, Martin (referee) ; Jiřík, Radovan (advisor)
This diploma thesis focuses on the influence of myocardial fibrosis on cardiac activity, changes in end-diastolic and end-systolic volume, as well as changes in ejection fraction. The theoretical part is focused on studying the methods used in left ventricular segmentation. The goal is then to implement an algorithm suitable for left ventricular segmentation on the supplied dataset. Based on segmentation, the effect of myocardial fibrosis on the group of rats with induced fibrosis is monitored, at the same time the data are compared with the control group of healthy rats.
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Changes in elemental distribution of rat liver tissue associated with myocardial fibrosis using LA-ICP-MS
Štrenclová, Eliška ; Zlámalová Gargošová, Helena (referee) ; Vašinová Galiová, Michaela (advisor)
Myocardial fibrosis is a pathological condition characterized by excessive accumulation of extracellular matrix in cardiac tissue, resulting in impaired cardiac function and potentially serious cardiovascular complications. However, recent studies suggest that myocardial fibrosis may affect not only cardiac tissue but also other organs such as the liver. The aim of this bachelor thesis was to investigate changes in the elemental distribution of liver tissue from healthy individuals by comparing the elemental distribution of liver tissue from individuals with myocardial fibrosis using the LA-ICP-MS method. When comparing the distribution maps of the control and experimental groups, changes were observed mainly in the distribution of iron isotopes; therefore, particular attention was later paid to it. The results of this research may contribute significantly to a better understanding of the relationship between myocardial fibrosis and liver tissue, and the knowledge gained may help to develop new diagnostic approaches.
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Segmentation of Cardiac MR Images
Dohnalová, Petra ; Mézl, Martin (referee) ; Jiřík, Radovan (advisor)
This diploma thesis focuses on the influence of myocardial fibrosis on cardiac activity, changes in end-diastolic and end-systolic volume, as well as changes in ejection fraction. The theoretical part is focused on studying the methods used in left ventricular segmentation. The goal is then to implement an algorithm suitable for left ventricular segmentation on the supplied dataset. Based on segmentation, the effect of myocardial fibrosis on the group of rats with induced fibrosis is monitored, at the same time the data are compared with the control group of healthy rats.
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Study of the effect of myocardial fibrosis on elemental distribution in rat soft tissues using Laser Ablation ICP-MS
Machalová, Martina ; Zlámalová Gargošová, Helena (referee) ; Vašinová Galiová, Michaela (advisor)
The most common cause of death in the Czech Republic continue to be cardiovascular diseases. The field of heart failure therapy still keeps moving forward. Yet myocardial fibrosis is one of the consequences and causes of heart failure for which there exists no effective medical care. The solution would be predicting the advancement of fibrosis and a preventive therapy. Many medical methods are being developed now in this regard, and the element distribution in affected tissue could help them. That is the reason why this work centers on the research of the element distribution in rat hearts with an induced myocardial fibrosis. An influence of sample couloration on the analysis was observed during its course. Special attention was paid to the distribution of iron in the fibrotic tissue as well as to the comparisson of the images of myocardial fibrosis. It seems that the iron distribution specificly could be helpful in recognising a damaged myocardium.
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Impact of early postnatal period on pro-arrhytmogenic substrate development caused by pressure overload in rat heart
Zábrodská, Eva ; Olejníčková, Veronika (advisor) ; Kolesová, Hana (referee)
In adult heart, pressure overload leads to cardiac hypertrophy. Higher propensity of hypertrophied myocardium to life-threatening arrhythmia is attributed to structural, mechanical and electrical remodeling. Pro-arrhythmogenic remodeling comprise several factors depending on an experimental model and a stage of heart failure. This thesis aims to characterize the impact of these factors in our unique model of pressure overloaded neonatal rat heart. The constriction of abdominal aorta was performed at postnatal day 2 in male Wistar rats. Decreased body weight, significant since week 6, was observed during development of cardiomegaly. At 12 weeks, the heart to body weight ratio was increased by 45 % and by 109 % in group with compensated (AC I) and decompensated (AC II) heart failure, respectively. At this age, the ECG was recorded and histological and immunohistochemical measurements were performed to analyze the pro-arrhythmogenic remodeling of working myocardium and cardiac conduction system. The markers of pro-arrhytmogenic remodeling such as significant prolongation of QT and QTc intervals were observed in the ECG recordings of AC II animals. However, spontaneously occurring arrhythmias was not detected. Further analysis of working myocardium showed decrease in Cx43 expression and its...
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