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Secondary metabolite production in explantate cultures of St. John's worth
Křížová, Šárka ; Martin, Jan (advisor) ; Kašparová, Marie (referee)
Secondary metabolite production in explantate cultures of St. John's Wort Šárka Křížová Diploma thesis Charles University in Prague, Faculty of Pharmacy in Hradec Králové, Pharmacy Key words: St. John's Wort, elicitation, flavonoids, hydrogen peroxide, glutathion, neutral red. The goal of the diploma thesis was to influence the production of secondary metabolites (flavonoids) in explantate cultures of St. John's Wort. Method of elicitation was used. This method is based on adding of an elicitor (stressor) to the tissue culture. Suspensional and callus cultures of Hypericum Perforatum L. were used for experiments with potential elicitors: hydrogen peroxide, combination of hydrogen peroxide and Mg-ATP, glutathion and cellular pigment neutral red. Their effect to the production of flavonoids was evaluated after 4 and 24 hours. Cultures were cultivated on Murashige and Skoog medium with the addition of a growth hormone BAP and a growth stimulator α-NAO. HPLC method was used for analysis of the samples. Hydrogen peroxide raised the production of flavonoids, especially in suspensional cultures, in callus cultures the highest influence had glutathion (reduced form) and neutral red. The highest production of flavonoids was reached after 24 hours by addition of hydrogen peroxide in concentration 100 mg/l,...
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Selected schools' students' awareness of selected types of nutraceuticals
Homolková, Zuzana ; Váchová, Alena (advisor) ; Hanušová, Jaroslava (referee)
The main topic of this work is food supplements, their legislation and other theory connected with them, the main focus is on plant nutraceuticals, of which are chosen food supplements marketed to support immunity system - echinacea, sea buckthorn and oyster mushroom, food supplements to support psyche - St. John's wort, ginkgo and valerian and dietary supplements to support body fitness - tribulus, garcinia and psyllium. Subsequently, the topic focuses on the marketing of nutraceuticals, its modern ways and the possibilities of increasing the students' awareness in this field through the principles of media education. In the practical part, 112 students aged 15-19 are presented with a lecture presenting the topic and inviting students to raise their interest in the subject. The feedback of the lecture is provided by a questionnaire whose questions correspond to the hypotheses set by the author. KEYWORDS nutraceuticals, media education, marketing, Echinacea, Garcinia, Pleurotus, Hippophae, Gingko, Plantago, Hypericum, Valeriana, Tribulus
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Selected schools' students' awareness of selected types of nutraceuticals
Homolková, Zuzana ; Váchová, Alena (advisor) ; Hanušová, Jaroslava (referee)
The main topic of this work is food supplements, their legislation and other theory connected with them, the main focus is on plant nutraceuticals, of which are chosen food supplements marketed to support immunity system - echinacea, sea buckthorn and oyster mushroom, food supplements to support psyche - St. John's wort, ginkgo and valerian and dietary supplements to support body fitness - tribulus, garcinia and psyllium. Subsequently, the topic focuses on the marketing of nutraceuticals, its modern ways and the possibilities of increasing the students' awareness in this field through the principles of media education. In the practical part, 112 students aged 15-19 are presented with a lecture presenting the topic and inviting students to raise their interest in the subject. The feedback of the lecture is provided by a questionnaire whose questions correspond to the hypotheses set by the author. KEYWORDS nutraceuticals, media education, marketing, Echinacea, Garcinia, Pleurotus, Hippophae, Gingko, Plantago, Hypericum, Valeriana, Tribulus
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Secondary metabolite production in explantate cultures of St. John's worth
Křížová, Šárka ; Martin, Jan (advisor) ; Kašparová, Marie (referee)
Secondary metabolite production in explantate cultures of St. John's Wort Šárka Křížová Diploma thesis Charles University in Prague, Faculty of Pharmacy in Hradec Králové, Pharmacy Key words: St. John's Wort, elicitation, flavonoids, hydrogen peroxide, glutathion, neutral red. The goal of the diploma thesis was to influence the production of secondary metabolites (flavonoids) in explantate cultures of St. John's Wort. Method of elicitation was used. This method is based on adding of an elicitor (stressor) to the tissue culture. Suspensional and callus cultures of Hypericum Perforatum L. were used for experiments with potential elicitors: hydrogen peroxide, combination of hydrogen peroxide and Mg-ATP, glutathion and cellular pigment neutral red. Their effect to the production of flavonoids was evaluated after 4 and 24 hours. Cultures were cultivated on Murashige and Skoog medium with the addition of a growth hormone BAP and a growth stimulator α-NAO. HPLC method was used for analysis of the samples. Hydrogen peroxide raised the production of flavonoids, especially in suspensional cultures, in callus cultures the highest influence had glutathion (reduced form) and neutral red. The highest production of flavonoids was reached after 24 hours by addition of hydrogen peroxide in concentration 100 mg/l,...
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