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National Repository of Grey Literature 2 records found  Search took 0.01 seconds. 
Characterization of FRET sensor
 Datinská, Vladimíra ; Klepárník, Karel ; Belšánová, B. ; Minárik, M. ; Foret, František
In this study, we present characterization of sensor based on Fӧrster resonance energy\ntransfer (FRET). The sensor is composed of ssDNA chain attached to a laboratory\nsynthesized quantum dot (QD). A complementary chain of a sample is labeled by a\nluminescent dye. When the dsDNA hybrid is formed, the energy from the QD (donor)\nis transferred to the dye (acceptor) and FRET is observed as a decrease of QD\nluminescence emission intensity and an increase of dye luminescence emission\nintensity.
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Synthesis and analysis of quantum dot conjugates intended for fret sensor
 Datinská, Vladimíra ; Klepárník, Karel ; Belšánová, Barbora ; Minárik, M. ; Foret, František
Our work is focused on synthesis of sensor based on Fӧrster resonance energy transfer (FRET), which is aimed to be used for DNA mutation detection. The sensor is based on the attachment of laboratory synthesized quantum dot (QD) to ssDNA. A complementary chain of a sample is labeled by a fluorescent dye. Thus, only if dsDNA between both complementary chains is formed, the energy from QD (donor) can be transferr ed to the dye acceptor) and FRET is observed.
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