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High Voltage Pulse Generator for Electroporation of Cells
Puczok, Václav ; Martiš, Jan (referee) ; Červinka, Dalibor (advisor)
The main goal of this thesis is to design control board for the experimental electroporation device and to develop control firmware. The first chapter of this work focuses on the electroporation phenomenon itself. Behaviour of the cell model in external electrical field is described there as well as simulation and overview of how electroporation affects living tissue. It also explains the main requirements for parameters of the electroporation pulses as well as need for ECG synchronization. Furthermore, some remarks are given about novel high frequency electroporation method, which involves use of nanosecond bipolar high voltage pulse bursts. The second chapter briefly introduces commercial electroporation device called Nanoknife, including control part, power part, and it's limits. The third chapter consists of introduction of the novel experimental electroporation device developed at BUT. Power part of this device is discussed as well. Next chapter focuses on design of the control board for this device and also on description of the particular schematic parts. There is a control algorithm explanation in the fifth chapter of this thesis followed by the brief manual to machine operation.
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USB thermometer
Holub, Jiří ; Kubánek, David (referee) ; Lattenberg, Ivo (advisor)
This bachelor thesis deals with a problem of electrical measurement of temperature. It describes a various types of temperature sensors and processing data obtained from these. It describes in short the USB interface and its parameters. It also contains a design of hardware and software solution of thermometer using temperature sensor SMT 160, managed by microcontroller ATmega16 and communicating with PC via USB interface. Designed thermometer also offers possibility to save measured data to built-in memory and later load them from there. Measured data will be also displayed on LCD. Last part of these thesis is design of application for PC, which will serve for displaying measured data and adjustment of thermometer.
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Configurable SPI device
Ženčár, Pavol ; Věchet, Stanislav (referee) ; Krejsa, Jiří (advisor)
Táto práca sa zaoberá vývojom a testovaním konfigurovateľného SPI slave zariadenia, ktorého hlavným účelom je testovanie reálnych SPI master zariadení. Zariadenie je implementované pomocou STM32 mikrokontroléra na vývojovej doske NUCLEO. Medzi základné funkcionality zariadenia patrí meranie frekvencie SPI prenosu, stream mód, ktorý umožní umožní presne sledovať, čo pripojené master zariadenie posiela po SPI zbernici a LUT mód, ktorý umožňuje nakonfigurovať zariadenie tak, aby odpovedalo prednastavenými odpoveďami na aktuálnu príchodziu správu. Rozšírená funkcionalita sa skladá z EEPROM emulátor módu. V tomto móde sa zariadenia správa ako virtuálna náhrada reálneho EEPROM zariadenia. Zariadenie je pripojené k počítaču pomocou sériového portu a je možné toto zariadenie konfigurovať pomocou python programáterského rozhrania. Zariadenie taktiež hlási každú aktivitu na SPI zbernici tomuto python programátorskému rozhraniu.
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Configurable SPI device
Ženčár, Pavol ; Věchet, Stanislav (referee) ; Krejsa, Jiří (advisor)
Táto práca sa zaoberá vývojom a testovaním konfigurovateľného SPI slave zariadenia, ktorého hlavným účelom je testovanie reálnych SPI master zariadení. Zariadenie je implementované pomocou STM32 mikrokontroléra na vývojovej doske NUCLEO. Medzi základné funkcionality zariadenia patrí meranie frekvencie SPI prenosu, stream mód, ktorý umožní umožní presne sledovať, čo pripojené master zariadenie posiela po SPI zbernici a LUT mód, ktorý umožňuje nakonfigurovať zariadenie tak, aby odpovedalo prednastavenými odpoveďami na aktuálnu príchodziu správu. Rozšírená funkcionalita sa skladá z EEPROM emulátor módu. V tomto móde sa zariadenia správa ako virtuálna náhrada reálneho EEPROM zariadenia. Zariadenie je pripojené k počítaču pomocou sériového portu a je možné toto zariadenie konfigurovať pomocou python programáterského rozhrania. Zariadenie taktiež hlási každú aktivitu na SPI zbernici tomuto python programátorskému rozhraniu.
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USB thermometer
Holub, Jiří ; Kubánek, David (referee) ; Lattenberg, Ivo (advisor)
This bachelor thesis deals with a problem of electrical measurement of temperature. It describes a various types of temperature sensors and processing data obtained from these. It describes in short the USB interface and its parameters. It also contains a design of hardware and software solution of thermometer using temperature sensor SMT 160, managed by microcontroller ATmega16 and communicating with PC via USB interface. Designed thermometer also offers possibility to save measured data to built-in memory and later load them from there. Measured data will be also displayed on LCD. Last part of these thesis is design of application for PC, which will serve for displaying measured data and adjustment of thermometer.
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High Voltage Pulse Generator for Electroporation of Cells
Puczok, Václav ; Martiš, Jan (referee) ; Červinka, Dalibor (advisor)
The main goal of this thesis is to design control board for the experimental electroporation device and to develop control firmware. The first chapter of this work focuses on the electroporation phenomenon itself. Behaviour of the cell model in external electrical field is described there as well as simulation and overview of how electroporation affects living tissue. It also explains the main requirements for parameters of the electroporation pulses as well as need for ECG synchronization. Furthermore, some remarks are given about novel high frequency electroporation method, which involves use of nanosecond bipolar high voltage pulse bursts. The second chapter briefly introduces commercial electroporation device called Nanoknife, including control part, power part, and it's limits. The third chapter consists of introduction of the novel experimental electroporation device developed at BUT. Power part of this device is discussed as well. Next chapter focuses on design of the control board for this device and also on description of the particular schematic parts. There is a control algorithm explanation in the fifth chapter of this thesis followed by the brief manual to machine operation.
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