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Characterization of a defensin of the tick \kur{Dermacentor marginatus}
LEŠTINOVÁ, Kateřina
Antimicrobial peptides (AMPs), as a part of innate immune system of ticks and other living organisms, are able to eliminate pathogens. In ticks the most important group of AMPs is defensin family. In this work, defensin from the tick D. marginatus was studied. The defensin gene was isolated from D. marginatus fed females. Using RT-PCR the gene expression was detected in salivary glands and mitgut. Recombinant protein was expressed in the procaryotic expression system, purified and tested for its antimicrobial activity. Specific polyclonal rabbit antibodies (anti DR IgG) were prepared and tested for their specifity and sensitivity.
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Isolation and biochemical characterisation of FREP proteins from \kur{Dermacentor marginatus}.
ŠTĚRBOVÁ, Jarmila
Fibrinogen-related proteins (FREP) are lectins with possible functions in invertebrate immunity. Previously, a FREP protein from the hemolymph of the tick Ornithodoros moubata, Dorin M, was isolated and characterised in our laboratory. In this study, four FREP proteins named DMFREP1, DMFREP2, DMFREP3, and DMFREP4 were detected with anti-Dorin M antibodies in the hemolymph of the tick Dermacentor marginatus. These FREP proteins were characterised by biochemical methods, purified, antibodies directed against these proteins were produced and the proteins were localised to tick issues by immunofluorescence.
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