|
|
Application of Capillary Electrophoresis in Forensic Molecular Genetic Investigation
Grafnetterová, Anna ; Doubková, Klára (advisor) ; Coufalová, Pavla (referee)
5 Abstract The capillary electrophoresis (CE) is an analytical method based on the principle of the traditional plate electrophoresis. Modern ways of fluorescence marking and the detection result in the higher reliability of analyses as well as in more precise results. The utilization of this method is wide within a lot of science fields. In special cases this method can even replace methods used so far, i.e. HPLC. The CE is mainly used in the forensic investigation in particular for person identification based on the DNA analysis of the even minimal sample amount. The STR analysis is an example of such examination. It uses short tandem repeats with high variability within the population. Commercial companies e.g. Promega Corporation, Applied Biosystems etc., develop corresponding instrumentation, software and kits which are important for receiving correct data. As a final result it is obtained DNA profile evaluated by comparison of samples from the "crime scene" with the reference samples. Besides the using in the human domain the DNA profiles are often used in the veterinary practice, in animal (cats, dogs, horses, cattle etc.) raising or breeding, for improving the particular characteristics. Key words: capillary electrophoresis fluorescent labeling STR STR analysis DNA profil
|
|
|
Use of Biological Methods in Criminology
Müllerová, Nikola ; Pavlasová, Lenka (advisor) ; Ehler, Edvard (referee)
Criminology is a science dealing with the protection of citizens and state from infringement. Criminology uses mostly biological or genetic methods for crime detection. Forensic traces which are collected by forensic experts on the scene are the key items of those methods. Forensic genetics is among the most important forensic subdisciplines. Forensic genetics uses DNA analysis for identification. The main aims of this study are description and importance of biological, anthropological and genetic methods in criminology, different ways of forensic identification, division and collection of forensic traces, characterization and course of forensic DNA analysis and DNA profiling. Key words Criminology, forensic methods, forensic identification, forensic trace, forensic biology, anthropology and genetics, information systems, forensic DNA analysis, DNA profile.
|
|
|
Application of Capillary Electrophoresis in Forensic Molecular Genetic Investigation
Grafnetterová, Anna ; Doubková, Klára (advisor) ; Coufalová, Pavla (referee)
5 Abstract The capillary electrophoresis (CE) is an analytical method based on the principle of the traditional plate electrophoresis. Modern ways of fluorescence marking and the detection result in the higher reliability of analyses as well as in more precise results. The utilization of this method is wide within a lot of science fields. In special cases this method can even replace methods used so far, i.e. HPLC. The CE is mainly used in the forensic investigation in particular for person identification based on the DNA analysis of the even minimal sample amount. The STR analysis is an example of such examination. It uses short tandem repeats with high variability within the population. Commercial companies e.g. Promega Corporation, Applied Biosystems etc., develop corresponding instrumentation, software and kits which are important for receiving correct data. As a final result it is obtained DNA profile evaluated by comparison of samples from the "crime scene" with the reference samples. Besides the using in the human domain the DNA profiles are often used in the veterinary practice, in animal (cats, dogs, horses, cattle etc.) raising or breeding, for improving the particular characteristics. Key words: capillary electrophoresis fluorescent labeling STR STR analysis DNA profil
|
guest ::
