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Detection of the HLA-B27 allele using a commercial kit
VOLKOVÁ, Simona
The main topic of the presented work is the detection of the HLA-B27 allele using a commercially available kit from the company BAG HEALTH CARE. The HLA-B27 molecule has been linked to some diseases. The best known is the association with ankylosing spondylitis or Bechterew's disease. Ankylosing spondylitis is a lifelong disease, manifested by an inflammatory reaction mainly at the site of the vertebrae of the spine and large joints such as the hips, shoulders and knees. Thanks to the association, the detection of the HLA-B27 allele can be used as a molecular marker for early diagnosis, mitigation and improvement of the course of treatment of ankylosing spondylitis. The theoretical part of the thesis specifies ankylosing spondylitis and the main histocompatibility complex with emphasis on the key molecule HLA-B27. The practical part of the bachelor thesis is focused on sample collection, DNA isolation and DNA amplification. This is followed by the laboratory detection of the HLA-B27 allele itself using a commercial kit using gel electrophoresis to visualize DNA fragments. Thus, 20 anonymized DNA samples from volunteers were examined. Of the 20 samples examined, positivity for the HLA-B27 allele was demonstrated in 6 samples of volunteers who had different clinical manifestations of Bechterew's disease.
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Kinesiotherapeutic intervention focused on patients with Bechterew's disease
VONDRŮ, Jaroslav
As a topic of my bachelor thesis I chose "Kinesiotherapeutic intervention focused on patients with Bechterew's disease". I chose this topic, because of interest and deeper knowledge of extensive problems in category: degenerative diseases of the spine. Ankylosing spondylitis, as the disease is technically called, is relatively abundant in the population and for this reason it is in my interest to contribute, at least in part, to the sorting and definition of key informations related to this rheumatic disease. In the theoretical part of this work, I focus on basic knowledge about anatomical structures related to pathophysiology of this disease. Whereas this extensive industry includes bones, muscles and fascial systems. Further I describe basic characteristics of Bechterew's disease, diagnostic criteria, clinical course and possibilities of rehabilitation or pharmacological therapy. In the practical part I deal with qualitative research activity, which focuses on a group of three patients. At the very beginning, I describe the necessary obtaining of information in the form of medical history, anthropometry and orientation examination of mobility or muscle strength. The main goal was to create an effective therapeutic program, which consists of a combination of elements of several selected rehabilitation methodologies and active, regular kinesiotherapeutic intervention within the spa concept. Information about the performed therapy, used methodologies and procedures, I present in detail in the form of elaboration of several case studies. At the end of each of them, I wrote a summary output of therapy, which includes an evaluation of the therapeutic intervention. This work can be available to the professional public, physiotherapists, available physiotherapy students to gain a basic awareness of Bechterew's disease, its pathology and possible physiotherapeutic methods.
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Case report of physiotherapeutical care for a patient with diagnosis of ankylosial spondylitis
Krausová, Zuzana ; Opatrná Novotná, Irena (advisor) ; Hassmannová, Kristina (referee)
Author: Zuzana Krausová Title: Case report of physiotherapeutical care for a patient with diagnosis of ankylosial spondylitis Objectives: The aim of this bachelor thesis is to process the diagnosis of ankylosing spondylitis and on the basis of theoretical background to develop a case report of physiotherapeutic care for a patient with this disease. Methodes: The bachelor thesis consists of two parts - general and special. The general part includes information on the anatomy and kinesiology of the spine, the field of rheumatology, spondylarthritis and ankylosing spondylitis. This information was taken from professional publications and is cited in compliance with the valid citation standard. All cited sources can be found in the chapter entitled - List of used literature. The special part is processed in the form of a case report of physiotherapeutic care for a patient with ankylosing spondylitis. This part of the bachelor thesis contains input and output kinesiological analysis, detailed description of individual therapies and evaluation of the effect of therapy. Results: The results of physiotherapeutic care result from the comparison of input and output kinesiological analysis. The results are summarized in the chapter entitled - Evaluation of the effect of therapy. During the therapies, the pain...
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Case Study of Physiotherapy Treatment of the Patient with ankylosing spodylitis
Pradáčová, Lucie ; Kučerová, Ilona (advisor) ; Zinková, Iva (referee)
Title: Case study of physiotherapy treatment of the patient with ankylosing spodylitis. Objectives: The aim of this thesis was to analyze teoretical knowledge about ankylosing spondylitis and the development of case of a patient with ankylosing spondylitis. The special part contains a case of study of physical treatment of a patient with ankylosing spondylitis including complete kinesiology analysis, course of treatment and final evaluation. Case report has been prepared on the basic of experience in the Institute of Rheumatology in Peague the period 15th January - 9th February 2018. Summary: This bachelor thesis deals with ankylosing spondylitis and is divided into two parts. The general part contains etiology, diagnostics, clinical picture and its treatment. Keywords: ankylosing spondylitis, Bechterew disease, rheumatism, physiotherapy
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Laboratory methods using in diagnostic morbus Bechterew
FAROVÁ, Veronika
The aim of my bachelor thesis was to get familiar with laboratory methods, which are used in the diagnostics of morbus Bechterew. Very important for the diagnostics of morbus Bechterew is the marker HLA-B27, which is identified by screening, with the help of flow cytometer and then is the presence confirmed or refuted with the help of molecular-biological methods. Other laboratory examination, which is essential when it comes to interception and observation of the activities of morbus Bechterew, is the determination of the C-reactive protein level, which is an indicator of inflammatory reaction in the body of the patient. The last examination I got familiar with was the determination of the sedimentation speed of erythrocytes, which is used as an indicator of ongoing inflammation in the body. In the theoretical part of the thesis I focused primarily on the definition of morbus Bechterew. I dealt with the question what are the symptoms of the disease, then also according to which criteria is the disease diagnosed and what treatment is applied. In the second part of the theoretical part I focused primarily on the major histocompatibility complex, which is highly connected with morbus Bechterew. I dealt mostly with the structure and presentation of antigens by the means of MHC molecules of class I. I also mentioned the structure and the function of C-reactive protein. In the practical part I described laboratory methods used in diagnostics of morbus Bechterew. The first method I tried was screening examination of HLA-B27 marker by the means of flow cytometry. I introduced how the following processes should look like: collection of biological material, preparation of the sample and the analysis made on the flow cytometer. At the end of this part I put an example of the analysis results, how do the results look like when they are positive, negative, or in the grey zone. As a second method I introduced genetic typing of HLA-B27 molecule, which is made by the means of real time PCR. I mentioned the preanalytical part and the process of preparation. Moreover I dealt with the measuring of C-reactive protein by the means of nephelometry and then I also mentioned examination with the help of a test strip. The last method I tried was measuring the sedimentation speed of erythrocytes, I mentioned the principle of method, the process of measuring and the measured results as well. In the last part of my bachelor thesis I worked with the data I got from Motol University Hospital, more precisely from the Department of Immunology, 2nd Faculty of Medicine of Medicine, Charles University in Prague and Motol University Hospital. During the year 2017, overall 452 patients were tested on the presence of HLA-B27 marker and 75 of them tested positive. Then I worked only with HLA-B27 positive patients. I researched how much is the disease represented in both sexes, what age do the HLA-B27 positive patients have and what is their diagnosis.
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Case study of physiotherapy treatment of a patient with Ankylosing Spondylitis
Moravcová, Anna ; Nováková, Tereza (advisor) ; Taušová, Eva (referee)
Title: Case study of physiotherapy treatment of the patient with the diagnosis of ankylosing spondylitis. Summary of work: This bachelor's thesis tries to show a rheumatic disease ankylosing spondylitis. The theoretical part presents etiology, diagnostics, clinical picture and its treatment. The practical part focuses on physiotherapy treatment for patients with this disease. Case report has been prepared on the basis of experience in the Institute of Rheumatology in January 2016. Keywords: ankylosing spondylitis, rheumatism, Bechterew disease. Name of diagnosis: ankylosing spondylitis, Bechterew disease, M45. Powered by TCPDF (www.tcpdf.org)
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The use of assistive devices for patients with Bechterev disease
KULHÁNKOVÁ, Barbora
The theoretical part of the thesis describes the actual disease. It is a rheumatic disease which includes inflammatory processes located in the spinal area. The disease is also called Ankylosing spondylitis. Typical symptom of the Bechterew's disease is stiffness and consequent back pain. Successful treatment must include use of nonsteroidal antirheumatic drugs and pain-relievers. However, the most crucial element is regular physical training, which must not be avoided otherwise the client may become permanently disabled and need assistance. The successful treatment also in cludes repeated spa stays. The thesis also deals with the role of a nurse, who together with a professional team, including physiotherapists, ergotherapists and social workers, care for the client's needs as well as for his/her education and his/her family members' education. The nurse should emphasize the importance of regular physiotherapies and use of prescribed drugs to the patients with such diagnosis. The most crucial part of the thesis deals with compensation aids which allow the clients relieving pain when moving and which get the patients back in their common lives. The patients start to use the compensation aids, such as walking sticks, wheeled walkers, and hygienic aids with long handle, in advanced stages of the disease, when common movements are difficult and painful for them. Furthermore, I am writing about the possibility of renting or purchasing such aids. There are compensation-aid rental companies and shops which offer such aids. Another part of the thesis deals with compensation allowance, which the clients may receive from their insurance companies.It also describes changes in the patient's life and environment, which may occur after receiving the compensation aids. The major goal of the thesis is to describe the possibilities of usage of compensation aids by the Bechterew's patients. To reach that I have chosen two survey questions. The first one reveals the type of compensation aids the clients prefer using and the way how they get these. The latter question explores influence of the compensation aids to the clients' lives. The survey was done by qualitative research method. For this purpose, semistructured in-depth interview was conducted with clients who suffer from the Bechterew's disease and who use compensation aids for their everyday routines. The obtained information shows that the most used aids are those which we are typical for people with movement disorders. Mostly, the clients pay for their aids by themselves. Vouchers prescribed by doctors are only used to get the forearm crutches. The Bechterew's disease is still an incurable disease. It may have very serious consequences for the patients unless they execute regular physiothe rapy, repeated spa curative stays and use the medicaments. The thesis may be used as a source material for special brochures created for mobility-impaired clients.
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The influence of physiotherapy for patients with ankylosing spondylitis treated in outpatient facilities and spa resorts
HRADILOVÁ, Ivana
This Bachelor´s thesis is concerned with influence of physiotherapy at patients with Bechterew´s disease, out of them one group of research sample undergoes therapy in outpatient facilities and the second one undergoes all-embracing balneotherapy. In theoretical part is briefly described the stability of backbone, functional anatomy and kinezilogy of axial system. Next part is concerned to a detailed description of Bechterew´s disease, concerned with its categorization to the group of serum-negative spondylartritid, with definition and classification of the disease, its occurrence, genesis and progress, prognosis and clinical picture of the disease. The main aim of this thesis is to evaluate the effects of physiotherapy on patients with Bechterew´s disease who were treated in outpatient facilities and spa sanatorium. To achieve the goal was used the qualitative research, in which four participants took part, out of them two were treated outpatiently, namely in Rehabilitation and regenerative Borovany and in Outpatient clinic Medipont, s.r.o. Next two participants attended complex curative therapy in Berta´s spa Třeboň. The patients had different stadium of the disease in the range between II. V. stage of disability. During the introductory meeting was executed preliminary kinesiological examination, next they were asked to fill up the informed agreement and Functional index BASFI (The Bath Ankylosing Spondylitis Functional Index), which is used to considering the patient´s functional abilities to solve common daily activities and which reflects impact of the disease to the quality of patient´s life. After therapy´s ending it was executed final kinesiological analysis together with repeated filling out the Index, in order to evaluate success rate of the treatment in range of theirs functional skills. With participants were as well executed not structured interview, in which they communicate their subjective evaluation to the realized research and compared outpatient and spa therapy from their pont of view to their contribution to themselves. The results of preliminary and final researches and physiotherapy course are placed upon record in practical part of Bachelor´s thesis. The Bachelor´s thesis can be used as study material for students, medics and non-professional public as well to improve knowledge about influence of the physiotherapy at patients with Bechterew´s disease, as well to teach patients about possibilities of their treatment.
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Rheumatoid arthritis versus ankylosing spondylitis from the view of dependence to social network
JELENOVÁ, Michaela
The number of rheumatic diseases has been increasing in Europe. It is estimated that currently up to a quarter of Europeans suffer from some type of rheumatism. Rheumatoid arthritis and Bechterew's disease (ankylosing spondylitis) are chronic diseases manifested by pain, stiffness, inflammation of joints and the back. The theoretical section is divided into two parts; the first part defines rheumatoid arthritis and Bechterew's disease from the health point of view. The second part is devoted to the social area that is often neglected. The aim of the practical section of the thesis is to reveal how rheumatoid arthritis differs from Bechterew's disease in terms of the use of social assistance, as well as employability or participation in social life. For the data collection, qualitative research methods and interviewing techniques were used. The addressed respondents were patients of rheumatology consulting rooms of the Medipont Plus Ltd., who were divided into two groups ? patients with rheumatoid arthritis and those with Bechterew's disease. The research has shown that persons suffering form rheumatoid arthritis are more likely to get a disability pension than those with Bechterew's disease and they also more often use social contributions, most frequently the extra benefits for people with disabilities ? ZTP cards. This is related to a lower work capability in patients with rheumatoid arthritis compared to those with Bechterew's disease. The research results are influenced by the age of the respondents, the length of the illness, the difference in the roles of men and women and last but not least, by personality characteristics. The research has proved that patients suffering from rheumatic diseases as well as those suffering from Bechterew's disease and undergoing biological treatment are more self-sufficient due to this modern treatment. It has also been found that the respondents who are not fully self-sufficient are not informed about the possibilities to get social benefits and contributions and do not know where to get this information. The thesis could provide an impetus for the strategy when rheumatic diseases are not considered only a medical issue, because these diseases significantly influence the lives of the people affected and thus become a serious psychosocial issue.
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