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Antimicrobial substances from plants and their application in acne preparations
Alexová, Adéla ; Byrtusová, Dana (referee) ; Němcová, Andrea (advisor)
The aim of the bachelor thesis is a determination of selected active substances of various plants and their possible application in cosmetic products for acne. In the theoretical part, an inventory of active substances including their effects in cosmetic products has been developed. Furthermore, the work is focused on characteristics of certain types of plants, mechanisms of antimicrobial effects and the emergence and treatment of acne. In the experimental part of this work the contents of biologically active substances, antioxidant and antimicrobial activity in extracts from selected plant species are characterized, namely from flowers of Levandula angustifolia, Matricaria chamomilla, Calendula officinalis, Syzygium aromaticum, leaves of Salvia officinalis and leaves of Rosmarinus officinalis. The plant extracts are also tested in different mixtures. Plant extracts and mixtures are incorporated into a recipe of acne cosmetic products. Their inhibitory effect against Propionibacterium acnes which causes acne and against representatives G+, G- and yeast Micrococcus luteus, Escherichia coli and Candida glabrata is observed. From the results it is clear that the best products against acne are micellar water and possibly skin lotion. The biggest amount of active substances is in cosmetic products which contain Matricaria, Syzygium and Rosmarinus. Overall effect of active substances is lower in cosmetic products than in 100% extracts.
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Testing of the sensitivity of bacteria on selected substances in school environment
Švehlová, Vendula ; Pavlasová, Lenka (advisor) ; Skýbová, Jana (referee)
The aim of this bachelor thesis lies in describing and executing of methods which are determining the resistance of various types of bacteria to antibiotics and selected common household disinfectants and also selected products for oral hygiene. Due to the structure of these methods they can be used f.e. in practical classes in grammar or high schools. Antibiotic discs and other supplies are used when examining resistence of bacteria towards antibiotics which are affordable for aforementioned schools. We can see the efficiency of various antibiotics and disinfectants and we can easily decide which ones are worth of purchase and which ones are not very functional alltogether. Last items tested are samples of mouthwashes and toothpastes. The mechanisms behind the efficiency of all these substances and the sensitivity of bacteries will be described either in practical and theoretical part of this thesis. Key words: Antimicrobials substances, resistance, inhibition, agar soil, bacteria
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Antimicrobial substances from plants and their application in acne preparations
Alexová, Adéla ; Byrtusová, Dana (referee) ; Němcová, Andrea (advisor)
The aim of the bachelor thesis is a determination of selected active substances of various plants and their possible application in cosmetic products for acne. In the theoretical part, an inventory of active substances including their effects in cosmetic products has been developed. Furthermore, the work is focused on characteristics of certain types of plants, mechanisms of antimicrobial effects and the emergence and treatment of acne. In the experimental part of this work the contents of biologically active substances, antioxidant and antimicrobial activity in extracts from selected plant species are characterized, namely from flowers of Levandula angustifolia, Matricaria chamomilla, Calendula officinalis, Syzygium aromaticum, leaves of Salvia officinalis and leaves of Rosmarinus officinalis. The plant extracts are also tested in different mixtures. Plant extracts and mixtures are incorporated into a recipe of acne cosmetic products. Their inhibitory effect against Propionibacterium acnes which causes acne and against representatives G+, G- and yeast Micrococcus luteus, Escherichia coli and Candida glabrata is observed. From the results it is clear that the best products against acne are micellar water and possibly skin lotion. The biggest amount of active substances is in cosmetic products which contain Matricaria, Syzygium and Rosmarinus. Overall effect of active substances is lower in cosmetic products than in 100% extracts.
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Testing of the sensitivity of bacteria on selected substances in school environment
Švehlová, Vendula ; Pavlasová, Lenka (advisor) ; Skýbová, Jana (referee)
The aim of this bachelor thesis lies in describing and executing of methods which are determining the resistance of various types of bacteria to antibiotics and selected common household disinfectants and also selected products for oral hygiene. Due to the structure of these methods they can be used f.e. in practical classes in grammar or high schools. Antibiotic discs and other supplies are used when examining resistence of bacteria towards antibiotics which are affordable for aforementioned schools. We can see the efficiency of various antibiotics and disinfectants and we can easily decide which ones are worth of purchase and which ones are not very functional alltogether. Last items tested are samples of mouthwashes and toothpastes. The mechanisms behind the efficiency of all these substances and the sensitivity of bacteries will be described either in practical and theoretical part of this thesis. Key words: Antimicrobials substances, resistance, inhibition, agar soil, bacteria
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