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Conducting connections of ceramic with FR4 in 3D structure
Pelc, Miroslav ; Adámek, Martin (referee) ; Kosina, Petr (advisor)
This work deals with conductive connections ceramic substrate with FR4 into three – dimensional structure. High frequency amplifier was created on ceramic substrate as a concrete application, for check quality substrate on resulting behavior circuit. The supply voltage for this amplifier is provided by the DC voltage source developed for FR4. Further the thesis deals with quality verification of substances conductive connection and individual aspects, with affect resulting reliability of connection.
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The via interconnection in LTCC
Sedliak, Erik ; Klíma, Martin (referee) ; Kosina, Petr (advisor)
This thesis deals with manufacturing process of LTCC 3D structures. Emphasis is placed mainly on the formation of via interconnections. In the second section of thesis, different via interconnections were designed, created and tested. Aim of this thesis was to evalute impact of via properties on vias function.
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Web Server for Protein Structure Prediction
Votroubek, Lukáš ; Očenášek, Pavel (referee) ; Burgetová, Ivana (advisor)
This work deals with proteins, especially with their structure and kinds of tertiary, or 3D, structure prediction. Tertiary structure prediction is very important for function prediction of this vitally important substance. Bioinformatics do this prediction much more effective and faster, because classical methods of structure prediction directly from molecule are very expensive and slow. On the other hand they are much more exact. Objective of this thesis is to describe tertiary structure prediction methods, describe used tools and possibility of automatic communication with them. Next objective is describe implementation of server, that will serve to protein engineers for more effective finding of information about tertiary structure from more servers without requesting each of them separately. Results of testing will be described in this work too.
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Pressure sensor made 3D structure
Hanus, Martin ; Pekárek, Jan (referee) ; Kosina, Petr (advisor)
This thesis deals with the points at issue of a 3D structure pressure sensor. The pressure sensor converts pressure into another physical quantity which is easier measure and which is processed subsequently. Usually ways of pressure sensing is for example capacitive, strain-gauge and optical. The 3D structures are constructed through the use of a LTCC technology, which can be laminated to create multi-layer structures, compared to standard high-temperature ceramics. Passive circuit components can be applied onto particular ceramic layers using either thin-layer or thick-layer technology. Practice part of thesis deals with several ways of construction a pressure sensor. First of all capacitive, strain-gauge and optical sensor and possible problems with construction individual sensors above all problems with lamination, cavity or burning
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Effect of polymer type on the properties of 3D printed auxetic structures
Zbíral, Roman ; Štaffová, Martina (referee) ; Jančář, Josef (advisor)
This thesis deals with special structures such as optical, acoustic and mechanic metamaterials, which has their own specific properties. Auxetic materials as subgroup of mechanical metamaterials has its own special arrangement of sub-cells, that is bound to negative Poisson’s ratio. With mechanical load auxetic materials change its own density, geometry of structure and in thrust only change its internal structure instead of breaking. Most of these materials are made using additive technologies such as 3D print which allows better quality of produced structure and are made of special polymers which provide expected properties.
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Structural characterisation of Prp45 and its interaction partners
Kulhánková, Lucie ; Novotný, Marian (advisor) ; Kostovčíková, Klára (referee)
Prp45 is a SNW protein that is part of a spliceosome and therefore participates in splicing of pre-mRNA into mRNA. In spliceosome Prp45 joins as part of NTC complex before splicing reactions. Prp45 has several known interactional partners (such as small subunit of U2AF, Cyp2, Prp5, Prp22, Clf1 and Cwc3). These proteins serve many functions in the spliceosome from stability to regulation of splicing reactions. Spliceosome consists of several dozens of proteins and several RNA and undergoes massive structural changes during executing its function. Until recently very little was known about the spliceosome structure; however, recent studies allow us to look at interaction network of this machine. In this work we focused on identification of both previously predicted and new interactional partners of Prp45. Using available spliceosome structures we aimed to describe these proteins structurally and functionally. Key words: splicing, Prp45, 3D struktura, spliceosome, binding partners
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Regulation of cathepsin K activity by reactive inhibitory molecules applicable in biomedicine
Benýšek, Jakub ; Mareš, Michael (advisor) ; Obšilová, Veronika (referee) ; Kutil, Zsófia (referee)
3 Abstract Human cathepsin K (KatK) is a lysosomal cysteine protease expressed predominantly in osteoclasts. It is the most effective enzyme for collagen breakdown and its physiological function lies in the degradation of the extracellular bone matrix. Increased enzymatic activity of KatK is associated with osteoporosis, rheumatoid arthritis, and osteoarthritis. This makes KatK a target for the treatment of pathologies, and chemotherapeutics based on protease inhibitors are being developed for its regulation. This work deals with reactive peptidomimetic and low molecular weight inhibitors of KatK namely thiadiazoles, vinyl ketones, and cyanohydrazides. It focuses mainly on the determination of the binding mode of selective inhibitors and characterization of their key interactions with the active site of KatK. Crystallographic structural analysis was combined with approaches of computational chemistry, enzymological analysis, and cell-based assays to elucidate the relationship between structure and biochemical activity of the investigated inhibitors. The obtained results provide important information for the design and optimization of new highly effective and selective KatK inhibitors as potential drugs and diagnostic probes. Keywords: cathepsin K, protease, inhibitor, 3D structure, osteoporosis
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Effect of polymer type on the properties of 3D printed auxetic structures
Zbíral, Roman ; Štaffová, Martina (referee) ; Jančář, Josef (advisor)
This thesis deals with special structures such as optical, acoustic and mechanic metamaterials, which has their own specific properties. Auxetic materials as subgroup of mechanical metamaterials has its own special arrangement of sub-cells, that is bound to negative Poisson’s ratio. With mechanical load auxetic materials change its own density, geometry of structure and in thrust only change its internal structure instead of breaking. Most of these materials are made using additive technologies such as 3D print which allows better quality of produced structure and are made of special polymers which provide expected properties.
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Structural characterisation of Prp45 and its interaction partners
Kulhánková, Lucie ; Novotný, Marian (advisor) ; Kostovčíková, Klára (referee)
Prp45 is a SNW protein that is part of a spliceosome and therefore participates in splicing of pre-mRNA into mRNA. In spliceosome Prp45 joins as part of NTC complex before splicing reactions. Prp45 has several known interactional partners (such as small subunit of U2AF, Cyp2, Prp5, Prp22, Clf1 and Cwc3). These proteins serve many functions in the spliceosome from stability to regulation of splicing reactions. Spliceosome consists of several dozens of proteins and several RNA and undergoes massive structural changes during executing its function. Until recently very little was known about the spliceosome structure; however, recent studies allow us to look at interaction network of this machine. In this work we focused on identification of both previously predicted and new interactional partners of Prp45. Using available spliceosome structures we aimed to describe these proteins structurally and functionally. Key words: splicing, Prp45, 3D struktura, spliceosome, binding partners
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Structural basis of cell invasion
Hrčkulák, Dušan ; Novotný, Marian (advisor) ; Hájková, Zuzana (referee)
Mezenchymal migration strategy is a mode of individual cell invasion, along with an ameboid migration strategy. It is dependent on cell adhesion structures formation and traction forces generation. This work is focused on integrin-mediated cell to extracellular matrix connections called focal adhesions. Focal adhesions are very complex and comprise of many proteins. The clusters of integrin dimers make up the focal adhesion core that binds extracellular matrix. Their intracellular domains indirectly interact with actin stress fibres throught plaque proteins talin and vinculin. Focal adhesion assembly is force dependent and its commponents turnover is also regulated by focal adhesion kinase and prolin-rich tyrosin kinase 2. These kinases are probably recruited to focal adhesions by paxillin and then linked to signaling complexes by adaptor proteins as p130Cas. The 3D structure is what defines the options of interaction among participating proteins. Therefore, this work summarizes 3D structures of six proteins of interest, deals with their interactions and impact on focal adhesions. PDB codes of all available structures of these six proteins are enclosed.
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