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National Repository of Grey Literature

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Stanovení obsahu karotenoidů u vybraných druhů mikrozeleniny Otrusinová, Kateřina The thesis focuses on the content of carotenoids (β-carotene and lutein) in 14 species of microgreens. Although microgreens are small in size, they are distinctive in taste, colour and health benefits. The results showed that different types of microgreens contain very different amounts of β-carotene and lutein. The β-carotene content ranged from 1.55–4.82 mg / 100 g fresh microgreens, while the lutein content ranged from 2.33–8.01 mg / 100 g fresh microgreens. Comparing the measured values in microgreens with the concentrations of these carotenoids in mature vegetables found in the literature, microgreens are often a richer source of β-carotene and lutein. Since carotenoids are biologically active substances with strong antioxidant capacity, the comparison of the content of these pigments in different types of microgreens is essential for the food industry and for the consumers. Detailed record

Development of HPLC separation of astaxanthin, β-carotene and lutein Hrubcová, Kateřina ; Nováková, Eliška (advisor) ; Kozlík, Petr (referee) This bachelor's thesis deals with the development of a method for the separation of carotenoid pigments, especially astaxanthin, β-carotene and lutein, produced by the microscopic green alga Tetracystis pulchra. The work compares published methods for the separation of carotenoid pigments and attempts to optimize a method suitable for the analysis of pigment extracts from Tetracystis pulchra cells. Optimized conditions for HPLC-DAD separation of carotenoid pigments were as follows: mobile phase A - ACN/MeOH/0,05M Tris-HCl buffer (pH = 8,0): 94/2/4, mobile phase B - MeOH/EtOAc: 68/32 under gradient elution, flow rate 0,25 ml min˗1 , column temperature 28 řC. The column used was Kinetex C18 (100 × 2,1 mm, 2,6 µm), injection volume was 20 µl and UV detection was performed at 470 nm. Total analysis time was 23 minutes. Key words: HPLC, astaxanthin, β-carotene, lutein Detailed record

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