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Trypanosomes from the T. theileri group in ungulates in the Czech Republic
Brotánková, Anna ; Brzoňová, Jana (advisor) ; Juránková, Jana (referee)
The Trypanosoma theileri group are blood parasites of ungulates. Confirmed vectors of these protists are horseflies or sheep keds for T. melophagium, but these trypanosomes were also detected in deer keds, mosquitoes and phlebotomus. We targeted on the investigation of possibility mosquitoes acting as vectors and additionally we targeted on the prevalence of T. theileri in mosquitoes at selected locations in the Czech Republic due to previous positive detection there. The measured prevalence has reached 6,4 % in mosquitoes, 4 % in deer keds and 16,7 % in sheep keds. The aim of experimental infections was to discover a potential of mosquitoes and phlebotomus acting as vectors of the T. theileri. Phlebotomus perniciosus and three species of mosquitoes Culex pipiens molestus, Culex pipiens quinquefasciatus and Aedes vexans were used for those experiments. Furthermore, isolates of T. theileri and T. melophagium from different sources were used. The divergence among these isolates and among used species of insect was found. The best results were achieved with Ae. aegypti and isolates from mosquitoes, where prevalence had reached 90,8 %. The infected mosquitoes were used for prediuresis research which had shown infection forms of the trypanosomes in the urine liquid. The phylogenetic analyse of T....
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Diversity, ecology and ecophysiology of snow algae
Mikešová, Lenka ; Nedbalová, Linda (advisor) ; Kvíderová, Jana (referee)
Permanent and temporary snow cover in polar and mountain areas is a very extreme habitat. However, there are organisms that inhabit this environment. Green algae of the order Chlamydomonadales (Chlorophyta) belong among the important representatives of cryoseston, which are known from polar and mountain regions around the world. These organisms, which occupied the snow environment probably secondarily, developed different ecophysiological adaptations required for the successful survival in extreme conditions. Low temperatures and high levels of radiation are the main extreme factors that organisms must cope with. The most important survival mechanisms include the adaptation of life cycles (such as of the shifts between resistant stages and moving vegetative stages), accumulation of secondary carotenoids, changes in fatty acid composition of membranes and lowering of growth temperature optima in some species. These adaptations together with the study of diversity represent at present the main subjects of research of snow algae.
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Trypanosomes from the T. theileri group in ungulates in the Czech Republic
Brotánková, Anna ; Brzoňová, Jana (advisor) ; Juránková, Jana (referee)
The Trypanosoma theileri group are blood parasites of ungulates. Confirmed vectors of these protists are horseflies or sheep keds for T. melophagium, but these trypanosomes were also detected in deer keds, mosquitoes and phlebotomus. We targeted on the investigation of possibility mosquitoes acting as vectors and additionally we targeted on the prevalence of T. theileri in mosquitoes at selected locations in the Czech Republic due to previous positive detection there. The measured prevalence has reached 6,4 % in mosquitoes, 4 % in deer keds and 16,7 % in sheep keds. The aim of experimental infections was to discover a potential of mosquitoes and phlebotomus acting as vectors of the T. theileri. Phlebotomus perniciosus and three species of mosquitoes Culex pipiens molestus, Culex pipiens quinquefasciatus and Aedes vexans were used for those experiments. Furthermore, isolates of T. theileri and T. melophagium from different sources were used. The divergence among these isolates and among used species of insect was found. The best results were achieved with Ae. aegypti and isolates from mosquitoes, where prevalence had reached 90,8 %. The infected mosquitoes were used for prediuresis research which had shown infection forms of the trypanosomes in the urine liquid. The phylogenetic analyse of T....
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Diversity, ecology and ecophysiology of snow algae
Mikešová, Lenka ; Nedbalová, Linda (advisor) ; Kvíderová, Jana (referee)
Permanent and temporary snow cover in polar and mountain areas is a very extreme habitat. However, there are organisms that inhabit this environment. Green algae of the order Chlamydomonadales (Chlorophyta) belong among the important representatives of cryoseston, which are known from polar and mountain regions around the world. These organisms, which occupied the snow environment probably secondarily, developed different ecophysiological adaptations required for the successful survival in extreme conditions. Low temperatures and high levels of radiation are the main extreme factors that organisms must cope with. The most important survival mechanisms include the adaptation of life cycles (such as of the shifts between resistant stages and moving vegetative stages), accumulation of secondary carotenoids, changes in fatty acid composition of membranes and lowering of growth temperature optima in some species. These adaptations together with the study of diversity represent at present the main subjects of research of snow algae.
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