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Methodology for co-cryostorage of virus isolates in their host plants and relevant data
Bilavčík, A. ; Bjerkestrand, R. ; Bjelland, B. ; Blystad, D.-R. ; Bobrova, O. ; Fránová, Jana ; Faltus, M. ; Hamborg, Z. ; Hammond Hammond, S. D. ; Koloniuk, Igor ; Zámečník, J.
Currently, the nursery production of plant material emphasizes the production of healthy, virus-free material. For this reason, it is necessary to have reference plant materials in which the virus is present. Some types of viruses are difficult to maintain outside the plant host. Because of the potential transmission to outdoor plantings, it is necessary to keep infected plants in special cultivation areas, and net-houses, which brings considerable financial and labour costs. Therefore, a methodology was developed in which the virus status in plant material is analysed, the infected material can be stored using cryopreservation in liquid nitrogen and then regenerated in vitro, and relevant data in GeneEver cryobox can be stored.
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Virus eradication method by cryopreservation tool - a cryoknife
Bilavčík, A. ; Bobrova, O. ; Faltus, M. ; Fránová, Jana ; Hammond, S. ; Koloniuk, Igor ; Zámečník, J.
Currently, in the nursery production of plant material, emphasis is placed on the propagation of healthy material. For this reason, it is necessary to pay attention to the topic of healthy virus-free nuclear stock when preserving collections of genetic resources of vegetatively propagated plants. Some raspberry viruses are difficult to remove from the plant host by existing methods of thermotherapy or chemotherapy. That is why, using the example of black raspberry necrosis virus (BRNV), a comprehensive methodology for testing health status and cryotherapy in raspberry was developed. The resulting methodical procedure makes it possible to eradicate BRNV from raspberry using cryotherapy of in vitro cultures. The user of the methodology is the Ministry of Agriculture, which applies it as part of the 'National Program for the Conservation and Use of Genetic Resources of Plants, Animals and Microorganisms Important for Nutrition and Agriculture'.
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Business Project
Malý, Milan ; Zámečník, Josef (referee) ; Groligová, Ivana (advisor)
The aim of this diploma work is to elaborate the proposal of conditions and arrangements of the scheme ralating the analyses of the environment, management and financial analyses in the company. On the basis of this analyses there are carried out the measurements and recommendations for improving the stability and profitability ot the company TIPA, Ltd. Finaly it is intended to use these analyses to improve the company´s position in the market.
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Fundraising as a Way of Financing Nonprofit Organizations
Zámečník, Jan ; Vechetová, Marta (referee) ; Koleňák, Jiří (advisor)
Master's thesis comprehends informations about ways of financing nonprofit organization and about possible sources, that are available in fundraising. There is also list of important givers in last years. Next part of thesis comprehends informations about activities, that would be made by nonprofit organizations before appeal for grant. There are also informations about activities, that follow after pick up grant. Part of thesis presents ways of gain financial sources in nonprofit organization signály.cz, o.s.
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Marketing Mix Proposal in the Services Area
Havlíčková, Barbora ; Zámečník, Jan (referee) ; Novák, Petr (advisor)
This master´s thesis deals with the assessment of the current state of the marketing mix business entity providing manicure and pedicure services and suggestions for improvement. In the theoretical section provides the theoretical background associated with the problem. analytical part deals with basic information about the business entity application marketing analysis and evaluation of the survey. The practical part is based on the analysis results and the survey deals with a recommendation proposing changes to the marketing mix to ensure widespread awareness on services of the business entity.
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Comparison of assessment before and after reform with extra sight on the PIT
Zámečník, Jan ; Dedková, Marta (referee) ; Kopřiva, Jan (advisor)
This bachelor´s thesis deal with reform of public financies. It is focusing principally on changes in the personál income tax. It contains examples, that can be practically used in life. It contains detailed description of contemporary and coming wals how to calculate the tax. Part of the bechelor‘ thesis is also about common identification with taxes in Czech republic and basic duties for tax payers. One section is also about other parts of reform of public finances. This section is in particular about health aod social part.
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Modern Sollution of the Family House Computer Network
Moravec, Lukáš ; Zámečník, Jan (referee) ; Ondrák, Viktor (advisor)
This bachelor thesis deals with the design of computer networks for a house using an automated household, included heat generation, smoke detection, lights and many others. First part is based on the analysis of the house, requirements and possibilities of the client. The second part focuses on the theoretical bases. And the final part covers the concept of the solution and the specified procedures and elements, which will be used for realization.
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