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Toxocara canis
Žáková, Barbora ; Voxová, Barbora (advisor) ; Vejsová, Marcela (referee)
The aim of this work is to compare seroprevalence against Toxocara of patients from FN HK between 2017 and 2021. Finally these results are compared with the results of the study of PharmDr. Barbora Voxová and Čermáková from 2009-2013. The methods of investigation were chosen indirect serological. It was testing by ELISA methods on present of IgG antibodies in the serum in both of studies (from 2009-2013 and 2017-2021). If the presence of antigen in serum was confirmed, an avidity test was made. This avidity test was made by modified ELISA method using of urea. During period 2017 to 2021 a total of 609 patients were examined (282 men and 327 women). The largest group of patients consisted of patients aged 50-60, the smallest group of patients was over 80 years. 7 % of patients had positive result (47 % were men and 53 % were women). The most positive (11) were in the age group 70-80 years, the least (0) in the age group 0-10 years. 31 % of positive had low avidity, 8 % had borderline avidity and 61 % had high avidity. During period 2009 to 2013 a 1083 patients were examined. 10 % of patients had positive result (53 % were men and 47 % were women). 11 % positive had low avidity and 89 % had high avidity. The number of examinations decreased rapidly between the two studies. In theyears 2009-2013 there...
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Evaluation of antifungal activity of newly synthesized compounds
Bláhová, Lenka ; Voxová, Barbora (advisor) ; Konečná, Klára (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Biological and Medical Sciences Lenka Bláhová Supervisor: PharmDr. Barbora Voxová Thesis title: Evaluation of antifungal activity of newly synthesized compounds English title: Evaluation of antifungal activity of newly synthesized compounds Thesis goal The goal of the thesis is to test new molecules as for their antifungal activity. Altogether 28 compounds of natural origin from the Department of Pharmaceutical Botany of the Faculty of Pharmacy of the Charles University were tested. Tested was the sensitivity to potentially antifungal substances with 8 micromycete strains, namely Candida albicans, Candida krusei, Candida parapsolosis, Candida tropicalis, Aspergillus fumigatus, Aspergillus flavus, Absidia corymbifera, Trichophyton interdigitale. Methods Molecules were tested with the broth microdilution method. Results 28 newly synthesized compounds were put to the test to prove the antimycotic effect. 11 out of them proved to be hopeful for further testing. Antifungal activity against all testing micromycetes was found with 5 compounds, 6 compounds proved antifungal activity against at least one micromycete. Conclusion The molecule structure of effective and ineffective compounds differs in the substitution of the benzene...
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Pathogenic Escherichia coli
Bílková, Dominika ; Vejsová, Marcela (advisor) ; Voxová, Barbora (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biological and Medical Sciences Subject of study: Healthcare bioanalytics Author: Dominika Bílková Supervisor: Mgr. Marcela Vejsová, Ph.D. Title: Pathogenic Escherichia coli Background: The aim of this work is to gather basic knowledge about bacteria Escherichia coli. The work is mainly focused on pathogenic serotypes of E. coli, their virulence factors and symptoms of diseases. The work also deals with laboratory diagnosis options for E. coli and treatment and prevention options. Main findings: Escherichia coli is a Gram-negative, facultative anaerobic bacterium and a member of the family Enterobacterales. It is common part of the human intestinal microflora, where it produces vitamin K and inhibits the growth of pathogenic bacteria. Some serotypes of E. coli are pathogenic and they have the ability to cause intestinal or extraintestinal diseases. Intestinal pathogens cause intestinal infections with diarrhea and they are divided into enteropathogenic (EPEC), enterotoxigenic (ETEC), enterohemorrhagic (EHEC), enteroaggregative (EAEC) and diffusely adherent (DAEC) E. coli. Extraintestinal pathogens cause mainly urinary tract infections, neonatal meningitis and sepsis. Conclusions: Escherichia coli is important part of...
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Chlamydia - the old-new diagnostic and therapeutic problem in human medicine.
Šifaldová, Lenka ; Jílek, Petr (advisor) ; Voxová, Barbora (referee)
The bachelor work is focused on diagnosis and treatment infections, which are caused by Chlamydia bakteria and Chlamydophila bakteria important in human medicine. It is Chlamydia trachomatis a common caused of sexually transmited infections and Chlamydophila pneumoniae beggetter of pneumonia. In the theoretical part of the work present morfology, biology findings and antigen set of different types of chlamydia bacteria are summarized. It is payed attention to life cycle, pathogenicyty and immunity system reaction. It is hard to recognize these infections because of different clinical manifestations and covering them in other diseases. Physician awareness in this problem and laboratory diagnosis is still limited. Therefor, the course of the disease is complicated, it can usually contribute to the chronicity and cause to serious impact as woman infertility. The aim of this work is also description and treatment options of most common infections. In the practical part of this work existing laboratory methods are described. Patient resultes of chlamydia infections, which were done in Central Laboratory SZZ Krnov during 2.1.2008 - 24.2.2010 , there are also presented.
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