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Isolation of compounds from Cannabis sativa and their anti-inflammatory effect
Baranová, Zuzana ; Vespalcová, Milena (referee) ; PharmDr.Daniela Veselá (advisor)
The object of this thesis is a traditional and also controversial plant Cannabis sativa. The theoretical part is focused on ubiquitous polyphenolic compounds – flavonoids, also present in the studied plant. All previously researched and confirmed effects on human body are described in detail. The experimental part of this thesis describes the isolation and identification of substances of the chloroform and hexane yields of extract obtained from this plant. Chromatographic methods were used for isolation – thin layer and column chromatography and semipreparative HPLC. Obtained substances were characterized using UV, IR spectrophotometry and NMR. Then the anti-inflammatory activity of obtained substances and also of the ethanolic extract and its yields was observed. The result of the experimental part was the isolation of one pure substance which was identified as the cannabidiolic acid. Using the THP 1 assay, we demonstrated mild anti-inflammatory effect of the non-polar yields and isolated CBDA.
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Isolation of compounds from Cannabis sativa and their anti-inflammatory effect
Baranová, Zuzana ; Vespalcová, Milena (referee) ; PharmDr.Daniela Veselá (advisor)
The object of this thesis is a traditional and also controversial plant Cannabis sativa. The theoretical part is focused on ubiquitous polyphenolic compounds – flavonoids, also present in the studied plant. All previously researched and confirmed effects on human body are described in detail. The experimental part of this thesis describes the isolation and identification of substances of the chloroform and hexane yields of extract obtained from this plant. Chromatographic methods were used for isolation – thin layer and column chromatography and semipreparative HPLC. Obtained substances were characterized using UV, IR spectrophotometry and NMR. Then the anti-inflammatory activity of obtained substances and also of the ethanolic extract and its yields was observed. The result of the experimental part was the isolation of one pure substance which was identified as the cannabidiolic acid. Using the THP 1 assay, we demonstrated mild anti-inflammatory effect of the non-polar yields and isolated CBDA.
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Spatial planning in Spain
Veselá, Daniela ; Damborský, Milan (advisor) ; Weingärtnerová, Markéta (referee)
The aim of diploma thesis is to determine advantages and disadvantages of spatial planning in Spain in comparison with the system of regional and physical planning in the Czech Republic. First, there will be briefly carry out the socio-economic characteristics of Spain which will serve as a starting point to a better understanding of land use. In sequence to this characteristics will be carry out the first structural part of the thesis, i.e. complex and comprehensive characterization and evaluation of spatial planning in Spain which will be examined, both in term of historical development and in term of the legislative, institutional and economic aspects. Then, the thesis will focus on the specific spatial planning problems solved in concrete selected regions of Spain. There will be briefly characterized system of regional and physical planning in the Czech Republic. The last and fundamental part of the thesis will focus on identifying advantages and disadvantages of the system of regional and territorial planning in the Czech Republic and spatial planning in Spain.
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