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Evaluation of activity of potentional antifungal substances through the use of microdilution broth method I.
Nitraiová, Jindřiška ; Paterová, Pavla (referee) ; Vejsová, Marcela (advisor)
1. Abstract Jindřiška Nitraiová Evaluation of activity of potentional antifungal substances through the use of microdilution broth method I Diplomová práce Univerzita Karlova v Praze, Farmaceutická fakulta v Hradci Králové Farmacie The aim of the thesis was a screening of activity of new potentional antifungal substances. For testing of substances was used the microdilution broth method. This method allows a routine assesment of MIC of a larger amount of antimycotics and also complete strains. The compounds for the strains Candida albicans, C. tropicalis, C. glabrata, C. krusei, Trichosporon beigelii, Aspergillus fumigatus, Absidia corymbifera a Trichophyton mentagrophytes were tested. The derivates of pyrazinedicarbamide, pyrazinedicarbothioamide, pyrazole-1- carboxylate, hydrazinecarboxylate, diazene-1,2-dicarboxamide and cyclohexanone did not show any antimycotic effectiveness. On the contrary the derivates of benzamide and benzenecarbothioamide showed a good antimycotic activity against all the testing strains. In the group of other substances, it means the derivates of pyrazinecarboxamide, pyrazinecarbothioamide, pyrazine-anilides, pyrazine-2-amine, pyrazine-2-carbohydrazide and pyrazine-2,5-dicarbonitrile and thiazolidine-4-one, some substances were effective but some had no effect. The derivates of...
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Evaluation of activity of potentional antifungal substances through the use of microdilution broth method II.
Rosíková, Pavla ; Paterová, Pavla (referee) ; Vejsová, Marcela (advisor)
Rosíková Pavla Evaluation of activity of potentional antifungal substances through the use of microdilution broth method II Diploma thesis Charles University in Prague, Faculty of Pharmacy in Hradec Kralove Pharmacy The aim of this diploma thesis was testing of samples of substances produced at the Department of Pharmaceutical Chemistry and Drug Control and the Department of Inorganic and Organic Chemistry Faculty of Pharmacy of Charles University in Hradec Kralove. Substances were tested by using microdilution broth method. Testing was carried out on eight strains of yeasts and filamentous fungi: Candida albicans, Candida tropicalis, Candida krusei, Candida glabrata, Trichosporon beigelii, Aspergillus fumigatus, Absidia corymbifera, Trichophyton mentagrophytes. Total were tested 49 substances, which are divided into four groups according to their chemical structure. Derivatives pyrazin-2-carboxamide, 3-derivatives hydrazinopyrazin-2- carboxamide, 2-derivatives isonikotinoylhydrazinkarboxylate, benzoxazin derivatives. Group of derivatives pyrazin-2-carboxamide, which is substituted by low alkyl (-CH3) in the position 5 of pyrazin, and in which is nitrogen of amid group subtituted by the phenyl with electronegative group (-CF3,-F,-Cl,-OH) or with hydroxy group in position m, showed higher...
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Evaluation of activity of potentional antifungal substances through the use of microdilution broth method II.
Fučíková, Eliška ; Křivčíková, Lucie (referee) ; Vejsová, Marcela (advisor)
Eliška Fučíková Evaluation of activity of potentional antifungal substances through the use of microdilution broth method II Diploma thesis Charles University in Prague, Faculty of Pharmacy in Hradec Kralove Specialist in laboratory methods Background: The aim of this thesis was to test substances with potential antifungal effect. We tested a totally 10 cathegories of substances: anilides of pyrazincarboxyl acid, arylaminopyrazines, pyrazin-2.3-dinitriles, pyrazincarboxyl acid esters, derivatives of (Z)-5-arylmethyliden-2-thioxothiazolidin-4-on, cholesterol and alkane acids esters, derivatives of thiosalicylamid , diamides, styrylbenzoxazoles, benzoxazepin-diones. Methods: The assesment was carried out using the microdilution broth method. Results: The most striking antifungal activity exhibit derivatives of thiosalicylamid for which MIC values were very low in almost all scale of concentration for all tested fungi. Conclusions: We found that the greatest antimycotic activity is manifested with the substitution of a halogen, mostly by chlorine.
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Evaluation of activity of potentional antibacterial substances through the use of microdilution broth method I.
Navrátilová, Kristýna ; Křivčíková, Lucie (referee) ; Vejsová, Marcela (advisor)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biological and Medical Sciences Study program: Pharmacy Candidate: Kristýna Navrátilová Supervisor: Mgr. Marcela Vejsová, Ph.D. Title of diploma thesis: Evaluation of activity of potentional antibacterial substan- ces through the use of microdilution broth method I Background Development of new antibacterial agents is still and will be always an impor- tant area of pharmaceutical industry. The need to discover new drugs is growing resistance of pathogenic bacterial strains. Aim of the work was to test new potencial antibacterial active substances on antimicrobial activity. Methods As part of this thesis was by using microdilution broth method tested for antibac- terial activity against eight chosen bacterial strains 106 chemicals newly synthesized by the Departments of the Faculty of Pharmacy in Hradec Králové. The described method is used as a screening for pre-determining of the effectiveness of antimicro- bial agents. Results Substances were divided into ten groups according to chemical structure. The most effective was reflected group of salicylthioamid derivates. These substances belong to this group inhibited bacterial growth of all tested strains by very low concentrations already. For each tested bacterial strain...
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Evaluation of activity of potentional antifungal substances through the use of microdilution broth method I.
Štvrtecká, Jana ; Vejsová, Marcela (advisor) ; Paterová, Pavla (referee)
Jana Štvrtecká Evaluation of activity of potentional antifungal substances through the use of microdilution broth method I Diploma thesis Charles University in Prague, Faculty of Pharmacy in Hradec Kralové Medical bioanalytic_____________________________________________________ The aim of this thesis is to evaluate the potencial in vitro antifungal activity of selected drugs synthetised on the Department of Pharmaceutical Chemistry and Drug Control and on the Department of Anorganic and Organic Chemismy on the Faculty of Pharmacy in Hradec králové. For drug testing was used the microdilution broth method. The tested compounds were tested on followed five yeast strains and three filamentous fungi models which are pathogenic for humans: Candida albicans ATCC 44859, Candida tropicalis 156, Candida krusei E28, Candida glabrata 20/I, Trichosporon beigelii 1188, Aspergillus fumigatus 231, Absidia corymbifera 272, Trichophyton mentagrophytes 445. 80 compounds were divided into 10 groups accorging to their chemical structures: anilides of pyrazin carboxyl acids, arylamin pyrazines, pyrazin carboxyl acid esters, pyrazine-2,3-dinitriles, benzoxazepin-diones, diamides, styryl benzoxazoles, derivatives of (Z)-5-arylmethyliden-2-thioxothiazolidin-4-on, ester derivatives of cholesterol and alcan acids and...
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