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Biological activity of Peganum harmala L. alkaloids and their semi-synthetic derivatives I.
Víchová, Barbora ; Chlebek, Jakub (advisor) ; Křoustková, Jana (referee)
1 ABSTRACT Víchová Barbora: Biological activity of Peganum harmala L. alkaloids and their semi-synthetic derivatives I. Diploma thesis 2022. Charles University, Faculty of Pharmacy in Hradec Králové Department of Pharmacognosy and Pharmaceutical Botany β-Carboline alkaloids are natural substances with potential use in tumor therapy. In particular harmine, which acts cytotoxically by several mechanisms. The aim of the diploma thesis was to prepare N9-derivatives of harmine derivatives, to clarify their structure based on common spectrometric and spectroscopic methods (ESI, ESI-MS, NMR) and subsequently to study their biological activity (cytotoxicity, AChE, BchE and GSk-3beta inhibition). These tests resulted in several active derivatives, suitable candidates for further study. 9-N-(2-nitrobenzyl)harmine (HMA-22) was significantly cytotoxic to the Jurkat cell line (16% cell viability after 48 hours, compared to the control, with better cytotoxicity than the standard doxorubicin at a concentration of 1 μM). Another very active derivative was found 9-N-(5-bromopentyl)harmine (HMA-30), which was more cytotoxic to most tested cell lines than doxorubicin (1 μM), similarly as most of other derivatives. HMA-30 was also found as a potent BChE inhibitor (IC 50 4.06 ± 0.48 μM). 9-N-(4-nitrobenzyl)harmine (HMA-20)...
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Legal aspects of environment protection within public procurement
Víchová, Barbora ; Sobotka, Michal (advisor) ; Drobník, Jaroslav (referee)
This work is focused on the "smart" purchasing from the point of view of considering the environmental criteria within this process - so called green public procurement. The aim of this work was a comprehensive describtion of possibilities pointing to environment protection within the scope of public procurement process, to contribute to knowledge of this mainly voluntary tool of environmental protection and to analyze it by the describtion of its evolution and contemporary state. This work provides an answer to a question how is the public procurement related to a environment protection, whether it is possible to protect the environment through a public procurement process and whether this kind of protection can be effective.
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Legal aspects of environment protection within public procurement
Víchová, Barbora ; Sobotka, Michal (advisor) ; Drobník, Jaroslav (referee)
This work is focused on the "smart" purchasing from the point of view of considering the environmental criteria within this process - so called green public procurement. The aim of this work was a comprehensive describtion of possibilities pointing to environment protection within the scope of public procurement process, to contribute to knowledge of this mainly voluntary tool of environmental protection and to analyze it by the describtion of its evolution and contemporary state. This work provides an answer to a question how is the public procurement related to a environment protection, whether it is possible to protect the environment through a public procurement process and whether this kind of protection can be effective.
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