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Electrolytic properties of hyaluronan solutions
Suchá, Šárka ; Smilek, Jiří (referee) ; Chytil, Martin (advisor)
This batchelor thesis deals with electrolytic properties of hyaluronan in aqueous solutions. In this study measuring of conductance, acid-base and conductrometric titration, and UV-VIS spectroscopy were used. The measuring of conductance was made on the solutions of different concentrations of hyaluronic acid and of different ionic strength. Acid-base titrations were performed in water solution and conductometric titrations were performed in 0,3mmol.dm–3 solution of sodium chloride. UV-VIS spectra of hyaluronic acid were measured in solution with addition of acid, base and in buffer solution. Values of pKa obtained from the above mentioned measurements was between 2,8–6,5.
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Investigation of acid-base and electrolytic properties of hyaluornan in aqueous solutions
Suchá, Šárka ; David, Jan (referee) ; Chytil, Martin (advisor)
This diploma thesis deals with acid-base and electrolytic behavior of hyaluronan solutions at different ionic strength. Acid-base behavior of hyaluronan was investigated by acid-base titrations which were carried out with two different methods, acid and alkaline acid-base titration. Dissociation constants at different ionic strength at zero degree of dissociation and at 50% degree of dissociation were evaluated from the results of acid-base titrations. Dissociation constants obtained from acid acid-base titrations have values between 3,0 and 3,6. Dissociation constants obtained from alkaline acid-base titrations are not very informative because their values are much higher than the expected values. The study of degradation of hyaluronan during acid-base titration was performed to complete study of acid-base behavior. Electrolytic behavior of hyaluronan solution was performed by conductometric titrations in three different environments.
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Investigation of acid-base and electrolytic properties of hyaluornan in aqueous solutions
Suchá, Šárka ; David, Jan (referee) ; Chytil, Martin (advisor)
This diploma thesis deals with acid-base and electrolytic behavior of hyaluronan solutions at different ionic strength. Acid-base behavior of hyaluronan was investigated by acid-base titrations which were carried out with two different methods, acid and alkaline acid-base titration. Dissociation constants at different ionic strength at zero degree of dissociation and at 50% degree of dissociation were evaluated from the results of acid-base titrations. Dissociation constants obtained from acid acid-base titrations have values between 3,0 and 3,6. Dissociation constants obtained from alkaline acid-base titrations are not very informative because their values are much higher than the expected values. The study of degradation of hyaluronan during acid-base titration was performed to complete study of acid-base behavior. Electrolytic behavior of hyaluronan solution was performed by conductometric titrations in three different environments.
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Electrolytic properties of hyaluronan solutions
Suchá, Šárka ; Smilek, Jiří (referee) ; Chytil, Martin (advisor)
This batchelor thesis deals with electrolytic properties of hyaluronan in aqueous solutions. In this study measuring of conductance, acid-base and conductrometric titration, and UV-VIS spectroscopy were used. The measuring of conductance was made on the solutions of different concentrations of hyaluronic acid and of different ionic strength. Acid-base titrations were performed in water solution and conductometric titrations were performed in 0,3mmol.dm–3 solution of sodium chloride. UV-VIS spectra of hyaluronic acid were measured in solution with addition of acid, base and in buffer solution. Values of pKa obtained from the above mentioned measurements was between 2,8–6,5.
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