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Development of HPLC method for determination of amiloride hydrochloride in pharmaceutical preparation
Sohrová, Barbora ; Matysová, Ludmila (advisor) ; Kujovská Krčmová, Lenka (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Analytical Chemistry Candidate: Barbora Sohrová Consultant: PharmDr. Ludmila Matysová, Ph.D. Diploma Thesis Title: Development of HPLC method for the determination of amiloride hydrochloride in medicinal preparation The purpose of thesis is the development of HPLC methods for determination of amiloride hydrochloride in pharmaceutical preparation. A medicinal product containing amiloride hydrochloride is used in the treatment of cystic fibrosis as a solution for inhalation. During the development, various stationary and mobile phases were tested. For the measurement was selected HS F5 column (150x 2,1 mm, 3 µm) and as the mobile phase was mixture of acetonitrile and 0,085% phosphoric acid to pH adjusted to 3,0 with triethylamine, in the ratio 75:25. Standard solution contained amiloride hydrochloride, Impurity A as a degradation product and butylparaben as an internal standard. The developed method was completely validated. According to results of the validation this method, was found that the developed method for the analysis of the product is suitable, and provides accurate and precise results. One of the findings of validation was the fact that the medicinal product containing amiloride hydrochloride is not stable...
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Development of HPLC method for the determination of homatropine and scopolamine in eye drops
Sohrová, Barbora ; Matysová, Ludmila (advisor) ; Kujovská Krčmová, Lenka (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Analytical Chemistry Candidate: Mgr. Barbora Sohrová Consultant: PharmDr. Ludmila Matysová, Ph.D. Thesis Title: Development of HPLC method for the determination of homatropine and scopolamine in eye drops High performance liquid chromatography (HPLC) method for the determination of homatropine hydrobromide and its degradation product- scopolamine hydrobromide in eye drops at a concentration of 1% (with sodium chloride), 2% (with sodium chloride) and 2% (with buffer F 6.45) active substance was developed. During the development of the method, various mobile and stationary phases were tested. For the validation was chosen column Ascentis Express F5 (100 x 4.6 mm, 2.7 µm). The mobile phase was composed of acetonitrile and phosphate buffer in a ratio of 9:91 (V/V). Phosphate buffer contained 3.12% aqueous solution of sodium dihydrogen phosphate dihydrate and 200 µl of triethylamine. Buffer pH was adjusted with phosphoric acid 85% to the value 2.5. Injection volume was 5 µl, flow rate was 1.1 ml/min and UV detection was performed at a wavelength of 210 nm. Analysis time was less than 6.5 minutes. Analyzed solution contained standard solution homatropine hydrobromide at a concentration of 25 mg/100 ml, and its degradation...
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Development of HPLC method for determination of amiloride hydrochloride in pharmaceutical preparation
Sohrová, Barbora ; Matysová, Ludmila (advisor) ; Kujovská Krčmová, Lenka (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Analytical Chemistry Candidate: Barbora Sohrová Consultant: PharmDr. Ludmila Matysová, Ph.D. Diploma Thesis Title: Development of HPLC method for the determination of amiloride hydrochloride in medicinal preparation The purpose of thesis is the development of HPLC methods for determination of amiloride hydrochloride in pharmaceutical preparation. A medicinal product containing amiloride hydrochloride is used in the treatment of cystic fibrosis as a solution for inhalation. During the development, various stationary and mobile phases were tested. For the measurement was selected HS F5 column (150x 2,1 mm, 3 µm) and as the mobile phase was mixture of acetonitrile and 0,085% phosphoric acid to pH adjusted to 3,0 with triethylamine, in the ratio 75:25. Standard solution contained amiloride hydrochloride, Impurity A as a degradation product and butylparaben as an internal standard. The developed method was completely validated. According to results of the validation this method, was found that the developed method for the analysis of the product is suitable, and provides accurate and precise results. One of the findings of validation was the fact that the medicinal product containing amiloride hydrochloride is not stable...
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Development of HPLC method for the determination of homatropine and scopolamine in eye drops
Sohrová, Barbora ; Matysová, Ludmila (advisor) ; Kujovská Krčmová, Lenka (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Analytical Chemistry Candidate: Mgr. Barbora Sohrová Consultant: PharmDr. Ludmila Matysová, Ph.D. Thesis Title: Development of HPLC method for the determination of homatropine and scopolamine in eye drops High performance liquid chromatography (HPLC) method for the determination of homatropine hydrobromide and its degradation product- scopolamine hydrobromide in eye drops at a concentration of 1% (with sodium chloride), 2% (with sodium chloride) and 2% (with buffer F 6.45) active substance was developed. During the development of the method, various mobile and stationary phases were tested. For the validation was chosen column Ascentis Express F5 (100 x 4.6 mm, 2.7 µm). The mobile phase was composed of acetonitrile and phosphate buffer in a ratio of 9:91 (V/V). Phosphate buffer contained 3.12% aqueous solution of sodium dihydrogen phosphate dihydrate and 200 µl of triethylamine. Buffer pH was adjusted with phosphoric acid 85% to the value 2.5. Injection volume was 5 µl, flow rate was 1.1 ml/min and UV detection was performed at a wavelength of 210 nm. Analysis time was less than 6.5 minutes. Analyzed solution contained standard solution homatropine hydrobromide at a concentration of 25 mg/100 ml, and its degradation...
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