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Interspecies comparison of plasma protein binding of two radiolabelled complexes
Smolová, Lenka ; Lázníček, Milan (advisor) ; Trejtnar, František (referee)
This paper engages in the newly prepared bifunctional chelate DTPA-oxn which has a potential use in radiolabelling biological macromolecules. The aim of the paper was to prepare a radiolabelled bifunctional chelate DTPA-oxn with a suitable radionuclide, the 90Y has been used. Binding of 90Y-DTPA-oxn to human, bovine, rabbit and rat plasma protein was determined. An equilibrium dialysis at 37řC was employed for the plasma protein binding determination. The received results received were compared with the results of plasma protein binding of a clinically routinely used bifunctional chelate DTPA radiolabelled with 99mTc (99mTc-DTPA). The results showed a low plasma protein binding of these radiolabelled chelates. Interspeices comparison demonstrated that the results obtained for human, bovine and rat plasma are comparable, whereas those obtained for rabbit plasma are higher. This brings a conclusion that the binding of 90Y-DTPA- oxn and 99mTc-DTPA to plasma protein is not a factor that can influence their biological behavior.
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Interspecies comparison of plasma protein binding of two radiolabelled complexes
Smolová, Lenka ; Trejtnar, František (referee) ; Lázníček, Milan (advisor)
This paper engages in the newly prepared bifunctional chelate DTPA-oxn which has a potential use in radiolabelling biological macromolecules. The aim of the paper was to prepare a radiolabelled bifunctional chelate DTPA-oxn with a suitable radionuclide, the 90Y has been used. Binding of 90Y-DTPA-oxn to human, bovine, rabbit and rat plasma protein was determined. An equilibrium dialysis at 37řC was employed for the plasma protein binding determination. The received results received were compared with the results of plasma protein binding of a clinically routinely used bifunctional chelate DTPA radiolabelled with 99mTc (99mTc-DTPA). The results showed a low plasma protein binding of these radiolabelled chelates. Interspeices comparison demonstrated that the results obtained for human, bovine and rat plasma are comparable, whereas those obtained for rabbit plasma are higher. This brings a conclusion that the binding of 90Y-DTPA- oxn and 99mTc-DTPA to plasma protein is not a factor that can influence their biological behavior.
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Study of Interspecies Differences in Plasma Protein Binding of New Radiopharmaceuticals
Smolová, Lenka ; Trejtnar, František (referee) ; Lázníček, Milan (advisor)
Study of interspecies differences in plasma protein binding of new radiopharmaceuticals Abstract This paper engages in a study on the plasma protein binding of chosen radiopharmaceuticals from the perspective of interspecies comparison. An equlibrium dialysis at 37řC was employed for the plasma protein binding determination and experiments were implemented on human, bovine, rabbit and rat plasma. Studies on the plasma protein binding were carried out with three new radiolabelled chelates and clinically routinely used bifunctional chelate DTPA radiolabelled with 99mTc (99mTc-DTPA). Binding was determined for the newly bifunctional chelate 90Y-DTPA-oxn and for the two potencional peptide based radiopharmaceuticals: 177Lu-DOTA-TATE a 177Lu-DOTA-MG-1. The results received were evaluated in light of interspecies comparison and compared with the results of the plasma protein binding of a clinically routinely used 99mTc-DTPA. Measuring data were analyzed using statistic data processing. The results of this analysis showed that statistically significant difference is for the complex 99mTc-DTPA between human and bovine and also between human and rabit plasma at the level of significance alfa 0,05. The results of all experiments carried out showed that the plasma protein binding is very low and therefore it is not a...
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