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Voltammetric detection of 7α-hydroxycholesterol on boron doped diamond electrode
 Serbanová, Lucie ; Schwarzová, Karolina (advisor) ; Nesměrák, Karel (referee)
This bachelor's thesis is dedicated to the electrochemical determination of 7α-hydroxycholesterol (7α-OHC) using boron-doped diamond electrode (BDDE). The work includes the optimization of experimental conditions and the measurement of the concentration dependence of 7α-OHC. The influence of various factors, such as water content in the solution and scan rate, on the electrochemical response of 7α-OHC was investigated. Various electrochemical methods, including cyclic voltammetry and differential pulse voltammetry, were used in the experiments. The electrochemical behavior of 7α-OHC in a solution of chloric acid and acetonitrile were analyzed. Important factors such as electrode passivation and response stability were carefully evaluated. The measurement of concentration dependence of 7α-OHC exhibited a linear response within the concentration range from 2∙10−5 mol l−1 to 1∙10−4 mol l−1 . Limits of detection (LOD) and quantification (LOQ) of 1,33 µmol l−1 and 4,43 µmol l−1 were determined. Comparison with other methods revealed that the electrochemical determination of 7α-OHC on BDDE achieves comparable sensitivity. The results of this work provide crucial insights into the electrochemical behavior of 7α-OHC, highlighting the advantages of BDDE for sensitive and reliable determination of...
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