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Possible usage of SVG and JavaScript for interactive maps
Stein, Zbyněk ; Čábelka, Miroslav (advisor) ; Schneider, Michal (referee)
The aim of this work is to demonstrate the possibilities of the SVG format and JavaScript in creating of interactive maps. The work is primarily focused on the creation of various elements of the digital interactive map. In the theoretical part of the work the attention is paid to the term of the interactive map and its integral components. The possibilities of use of both selected formats are described. The development of both formats and the way of their implementation are also mentioned. Other techniques used in the practical part are briefly listed. In the next part of the work the creation of the interactive map elements and the useability of the selected formats in practice are discussed and evaluated. The results are also demonstrated in the sample interactive map. Powered by TCPDF (www.tcpdf.org)
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Structural biochemistry of human NK cell receptor complex NKp30 and B7-H6
Skořepa, Ondřej ; Vaněk, Ondřej (advisor) ; Šulc, Miroslav (referee) ; Schneider, Bohdan (referee)
Natural killer cells (NK cells for short) are lymphocytes of the non-specific (innate) immune system. They excel in the ability to recognise and eliminate infected or cancerous cells rapidly. Although their role in immune surveillance of malignant transformation was confirmed years ago, ongoing research shows that this process is far from simple. NKp30 is one of the central activating receptors of NK cells with a potential for use in targeted immunotherapy. The oligomerisation of the extracellular ligand-recognition domain of NKp30 in solution depends on the presence of a C-terminal stalk region. However, the structure and role in signal transduction of these oligomers are still unclear. Moreover, the interaction of NKp30 with ligands is influenced by the presence of N-linked glycosylation. In this work, we investigated whether and how the oligomerisation of NKp30 depends on its glycosylation. Our results show that NKp30 forms oligomers, regardless of whether glycosylation is complex or uniform (acquired by expression in the HEK293S GnTI- cell line). In contrast, NKp30 was found to form only monomers when enzymatically deglycosylated. Furthermore, we characterised the interaction with the ligand B7-H6, again concerning oligomerisation and glycosylation. We solved the crystal structure of its...
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Increasing affinity of Interferon gamma receptor 1 to Interferon gamma by combining molecular modeling and experimental methods
Mikulecký, Pavel ; Schneider, Bohdan (advisor) ; Šulc, Miroslav (referee) ; Vaněk, Ondřej (referee)
Protein-protein interactions play an important role in nearly all processes of the living cells and the function of many proteins is dependent on their specific interactions with other biomolecules. A reliable tool to modulate these interactions would be invaluable for the development of molecules suitable for diagnostics, medicine, and biotechnology. In this work, we aimed to study the specificity of interactions in the model system of Interferon gamma receptor 1 (IFNgR1) and its natural ligand Interferon gamma (IFNg), important in innate immunity. We searched for mutations within the interferon receptor molecule IFNgR1 to modulate (increase as well as decrease) its affinity to IFNg by in silico analysis of the existing crystal structures of the complex between IFNgR1 and IFNg. We modeled amino acid substitutions and gauged how they influenced the interaction using empirical force field implemented in software FoldX. All selected promising IFNgR1 variants were expressed in Escherichia coli, purified to homogeneity, characterized, and kinetics of their interactions with IFNg was measured by Surface Plasmon Resonance (SPR). The first set of IFNgR1 variants included mutations on the interface of the IFNg/IFNgR1 complex. According to our SPR measurements, the affinity of most of these receptor...
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Possible usage of SVG and JavaScript for interactive maps
Stein, Zbyněk ; Čábelka, Miroslav (advisor) ; Schneider, Michal (referee)
The aim of this work is to demonstrate the possibilities of the SVG format and JavaScript in creating of interactive maps. The work is primarily focused on the creation of various elements of the digital interactive map. In the theoretical part of the work the attention is paid to the term of the interactive map and its integral components. The possibilities of use of both selected formats are described. The development of both formats and the way of their implementation are also mentioned. Other techniques used in the practical part are briefly listed. In the next part of the work the creation of the interactive map elements and the useability of the selected formats in practice are discussed and evaluated. The results are also demonstrated in the sample interactive map. Powered by TCPDF (www.tcpdf.org)
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