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Sjögren's syndrome - anamnestic study
Rosecká, Michaela ; Jílek, Petr (advisor) ; Janďourek, Ondřej (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biological and Medical Sciences Student: Michaela Rosecká Supervisor: PharmDr. Petr Jílek, CSc. Title of Bachelor thesis: Sjögren's syndrome - anamnestic study Backround: The aim of this work was to get acquainted with the problems of this serious illness and the comparison of the quality of life diagnosed in the Czech Republic and abroad. Main findings: Sjögren's syndrome is an autoimmune disease with exocrine gland disorders. The pathogenesis of this disease is still unclear, but knowledge of the involvement of various cells and the pathways leading to autoimmunity is increasing. The most common symptom is dryness of the eyes and mouth, but patients often have problems with musculoskeletal or systemic damage of the body. Sjögren's syndrome brings an increased risk of developing lymphoproliferative disease. Conclusions: Attempts to create new diagnostic criteria indicate the difficulty and great interest in understanding this disease. Accurate diagnosis should be performed by laboratory diagnostics in combination with appropriate tests. Still, it is a problem to identify this disease safely and Sjögren's syndrome remains an unknown and underestimated autoimmune disease.
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Monitoring of toxicological tests
Rosecká, Michaela ; Sklenářová, Hana (advisor) ; Horstkotte, Burkhard (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Analytical Chemistry Candidate: Bc. Michaela Rosecká Supervisor: Doc. PharmDr. Hana Sklenářová, Ph.D. Title of Diploma Thesis: Monitoring of toxicological tests The aim of this work was to monitor kinetic profiles of permeation of fluorescent marker through cell monolayer (MDCKII-MDR) by sequential injection analysis. For this purpose, a new type of permeation module was prepared that could measure from 2 compartments - donor and acceptor. The module was thermostated at 37řC during the experiment and liquid in the acceptor compartment was continuously agitated. Experiments were repeated to observe difference in interaction of fluorescent marker with cell membrane transporter. MDCKII-MDR monolayer cell inserts were replaced after the experiments. The cell line used in this experiment include a genetically modified P-glycoprotein. Rhodamine 123, the fluorescent agent, was applied for on-line monitoring of extracellular transport. The permeability kinetics of this marker was monitored using a fully automated sequential injection analysis system with five minutes sampling intervals for the testing period of 120 minutes. The sample was taken from the donor compartment in one cycle, and from the acceptor one in the next cycle. Then,...
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Monitoring of toxicological tests
Rosecká, Michaela ; Sklenářová, Hana (advisor) ; Horstkotte, Burkhard (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Analytical Chemistry Candidate: Bc. Michaela Rosecká Supervisor: Doc. PharmDr. Hana Sklenářová, Ph.D. Title of Diploma Thesis: Monitoring of toxicological tests The aim of this work was to monitor kinetic profiles of permeation of fluorescent marker through cell monolayer (MDCKII-MDR) by sequential injection analysis. For this purpose, a new type of permeation module was prepared that could measure from 2 compartments - donor and acceptor. The module was thermostated at 37řC during the experiment and liquid in the acceptor compartment was continuously agitated. Experiments were repeated to observe difference in interaction of fluorescent marker with cell membrane transporter. MDCKII-MDR monolayer cell inserts were replaced after the experiments. The cell line used in this experiment include a genetically modified P-glycoprotein. Rhodamine 123, the fluorescent agent, was applied for on-line monitoring of extracellular transport. The permeability kinetics of this marker was monitored using a fully automated sequential injection analysis system with five minutes sampling intervals for the testing period of 120 minutes. The sample was taken from the donor compartment in one cycle, and from the acceptor one in the next cycle. Then,...
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Sjögren's syndrome - anamnestic study
Rosecká, Michaela ; Jílek, Petr (advisor) ; Janďourek, Ondřej (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biological and Medical Sciences Student: Michaela Rosecká Supervisor: PharmDr. Petr Jílek, CSc. Title of Bachelor thesis: Sjögren's syndrome - anamnestic study Backround: The aim of this work was to get acquainted with the problems of this serious illness and the comparison of the quality of life diagnosed in the Czech Republic and abroad. Main findings: Sjögren's syndrome is an autoimmune disease with exocrine gland disorders. The pathogenesis of this disease is still unclear, but knowledge of the involvement of various cells and the pathways leading to autoimmunity is increasing. The most common symptom is dryness of the eyes and mouth, but patients often have problems with musculoskeletal or systemic damage of the body. Sjögren's syndrome brings an increased risk of developing lymphoproliferative disease. Conclusions: Attempts to create new diagnostic criteria indicate the difficulty and great interest in understanding this disease. Accurate diagnosis should be performed by laboratory diagnostics in combination with appropriate tests. Still, it is a problem to identify this disease safely and Sjögren's syndrome remains an unknown and underestimated autoimmune disease.
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