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National Repository of Grey Literature

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Polyphasic approaches in the taxonomy of green aerophytic algae Škaloud, Pavel ; Neustupa, Jiří (advisor) ; Lukešová, Alena (referee) ; Rindi, Fabio (referee) Conclusions Traditionaltaxonomyoftendependson single charactersusedfor distin- guishingparticularspecies.ln thisthesis,I haveillustratedapplicationofpoly- phasicapproachesin taxonomyof greenaerophyticalgae.Theseapproachesare basedon thecombinationof variouscharacters(traditionalmorphology,modern molphologicalapproaches,SSU, ITS rDNA andgeneintronsequencing)forthe speciesrecognitionin greenalgae. The morphologicalcharactersaloneareoftennot sufficientfor speciesde- lirnitation,thoughtheycan be usedfor routinespeciesidentificationModern rnorphologicalapproacheslike confocalmicroscopyallow to detectclearmor- phologicaldifferencesarnonginvestigatedcloselyrelatedspecies,evenifat first sightthesemorphologicallyvery uniformalgaehardlydifferatthe morphologi- cal level. A numberof rnorphologicalcharactersdoesnot reflectgeneticrela- tionships.Rathertheyareinfluencedby variousenvironmentalor culturecondi- tions.For example,cell dimensionsin Klebsorntidiuntcanbe distinctivelyinflu- encedby pH, humidity,illLrminationandternperatureconditions.However,with theaid of molecularmarkerswe candetectproperrnorphologicalfeaturesuseful for speciesdifferentiationt. ln .lslerochlorls,correlationtestof geneticdistances and morphologicaldifferencesrevealedusefulnessof particularchloroplast charactersforspeciesdelirnitation.... Detailed record

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