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Antimicrobial, cytotoxic, and antiprotease effects of silver used for treatment of infected wounds
Pavlík, Vojtěch ; Arenbergerová, Monika (advisor) ; Pospíšilová, Alena (referee) ; Resl, Vladimír (referee)
Chronic wounds pose a socioeconomic problem and burden to patients worldwide. There are several causes of wound chronicity. One of the major issues of the non-healing state of a wound is its infection. Bacteria provoke a proinflammatory response, which disrupts the proper sequence of wound healing phases. Also, inflammation raises the production and activity of proteases. These enzymes digest the extracellular matrix, resulting in deceleration or hindrance of granulation tissue maturation. In addition to the inflammation-induced proteases, some bacterial species produce proteases that contribute to tissue degradation and increase bacterial virulence. Antiseptics are used to lower the bacterial burden in chronic wounds. Antiseptics act non-specifically, which may be their asset as well as drawback. They act as a double-edged sword. Antiseptics act on bacterial cells. However, host cells are caught in the crossfire as antiseptics may damage eukaryotic cells as well. The damage of eukaryotic cells complicates wound closure. Silver is probably the most used antiseptic to treat chronic wounds. Silver is also suspected of inducing nonspecific damage to eukaryotic as well as prokaryotic cells. This doctoral thesis is aimed at investigating the multitude of effects that silver may exhibit. We chose in vitro...
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Evaluation of adjuvant treatment of malignant melanoma by real time RT-PCR
Gkalpakiotis, Spyridon ; Arenberger, Petr (advisor) ; Resl, Vladimír (referee) ; Pánková, Růžena (referee)
Melanoma is one of the tumors, characterized by considerable heterogeneity of expression of tumor-specific protein. Monitoring two or more markers can significantly increase the efficiency of detection. In our study, we concentrated to 5 marker: Melan-A/MART-1, gp 100, MAGE-3, MIA and Tyrosinase. Another potential tumor marker was telomerase. The most sensitive marker of progression proved to MAGE-3 (17 of 18 patients), followed by the positive marker gp100 (10 of 18 patients), MIA (9 of 18) and Tyrosinase (1 of 18). The Melan-A there was no statistically significant increase over the cutoff for all monitored patients with progression. Tyrosinase as a marker for circulating melanoma cells used in the past most frequently. Her role as a marker is highly debated and varies in different publications. For example The effectiveness of investigations Tyrosinase as the only marker of progression ranged from 6% to 59%. The reason may be technical error, a high percentage of false positives such as because of contamination or reduce expression Tyrosinase in advanced stages, which is associated with reducing the differentiation of tumor cells and decrease tumor melanization. Given that our work with progressive disease, most often found at the same time three positive markers, supports the detection of several marker...
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Evaluation of adjuvant treatment of malignant melanoma by real time RT-PCR
Gkalpakiotis, Spyridon ; Arenberger, Petr (advisor) ; Resl, Vladimír (referee) ; Pánková, Růžena (referee)
Melanoma is one of the tumors, characterized by considerable heterogeneity of expression of tumor-specific protein. Monitoring two or more markers can significantly increase the efficiency of detection. In our study, we concentrated to 5 marker: Melan-A/MART-1, gp 100, MAGE-3, MIA and Tyrosinase. Another potential tumor marker was telomerase. The most sensitive marker of progression proved to MAGE-3 (17 of 18 patients), followed by the positive marker gp100 (10 of 18 patients), MIA (9 of 18) and Tyrosinase (1 of 18). The Melan-A there was no statistically significant increase over the cutoff for all monitored patients with progression. Tyrosinase as a marker for circulating melanoma cells used in the past most frequently. Her role as a marker is highly debated and varies in different publications. For example The effectiveness of investigations Tyrosinase as the only marker of progression ranged from 6% to 59%. The reason may be technical error, a high percentage of false positives such as because of contamination or reduce expression Tyrosinase in advanced stages, which is associated with reducing the differentiation of tumor cells and decrease tumor melanization. Given that our work with progressive disease, most often found at the same time three positive markers, supports the detection of several marker...
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