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Canistherapy in a Retirement Home
PETRÁKOVÁ, Vendula
Canistherapy is an activity in which a dog has a positive effect on a person. It is a relatively new form of activation in retirement homes providing these people with many experiences that lead to the enrichment of their days. This bachelor thesis is focused on canistherapy in a retirement home. The goal of the thesis was to evaluate the benefit of canistherapy as a method in a retirement home. The thesis consists of two parts, a theoretical and practical. In the theoretical part I describe zootherapy. It also characterizes canistherapy, its forms, methods, the goal of canistherapy tests, contraindications of canistherapy and further it includes canistherapy in seniors. Positioning is not neglected and in the last part there is the activation of seniors, which is an integral part of working with seniors in residential facilities. The practical part consists of qualitative research. The method of semi-structured interview consisted of 9 questions was used to obtain data. The research group consisted of 6 communication partners from two retirement homes, who regularly participated in canistherapy for at least one year. The obtained data are evaluated by the method of open coding with subsequent categorization. The results of the research have shown that canistherapy is a beneficial method in all areas of the client's life, where I included the physical, social, and emotional aspect. The greatest benefit has been found on the emotional components of personality. I believe that this thesis can serve as an information material for future social workers or even for future canistherapists who would like to deal with this method in the future.
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Nanofiber dressing consisting of antisense rna-functionalized nanodiamonds for therapy of non-healing wounds in diabetic individuals
Neuhoferová, Eva ; Petráková, V. ; Vocetková, K. ; Kindermann, Marek ; Křivohlavá, Romana ; Benson, Veronika
Non-healing wounds are serious complication in diabetic patients and represent an attractive challenge for development of suitable carrier system possessing constant and localized release of therapeutic biomolecule into the wound without any undesired side effects. Given the fact that these non-healing wounds are result of impaired balance in metalloproteinases synthesized by immune cells residing the wounds, gene therapy offering knock down of such enzymes is of great interest. \nHere we challenged a development of functional and biocompatible wound dressing enabling controlled release of trackable carrier loaded with therapeutic siRNA. Our dressing consists of scaffold from degradable polymer nanofibers enriched with fluorescent nanodiamond particles (FND). We have previously shown the nanodiamond particles are great carriers for antisense RNAs. Their advantages represent high biocompatibility, stable luminescence giving us the possibility to track the carrier system in the wound, and effective release of antisense RNA in the wound. Embedding of nanodiamond-siRNA systems into nanofiber scaffold enables continuous release of siRNA and maintaining the stable siRNA concentration in the wound site resulting in a promotion of wound healing. \nWe developed FND-siRNA complexes specific to MMP-9 that efficiently inhibit the expression of target MMP-9 mRNA. The complexes were embedded into core/shell nanofibers from PVA and PCL, visualized by confocal microscopy, and characterized by electron microscopy. Real-time PCR was used to assess the silencing effect of siRNA that has been delivered to target murine fibroblasts by FND released from nanofiber dressing. Nanofiber system with embedded FNDs was applied on wounds in diabetic animal models to evaluate its suitability regarding short and long term toxicity, efficacy, and handling in vivo. \n
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Nanodiamonds as an innovative system for intracellular delivery of mirna-34a inprostatic cancer therapy
Bitti, G. ; Abate, M. ; Neuhoferová, Eva ; Kindermann, Marek ; Petráková, V. ; Boccellino, M. ; Quagliuolo, L. ; Filová, Eva ; Benson, Veronika ; Caraglia, M. ; Amler, Evžen
The microRNA(miRNA)-34a is an important regulator of tumor suppression. It controls the expression of several target proteins involved in cell cycle, differentiation and apoptosis, and antagonizes processes that are necessary for basic cancer cell viability as well as cancer stemness, metastasis, and chemoresistance. It is downregulated in numerous cancer types, including prostatic cancer, and inhibits malignant growth by repressing genes involved in various oncogenic signaling pathways. Given the anti-oncogenic activity of miR-34a, here we proved the substantial benefits of a new therapeutic concept based on nanotechnology delivery of miRNA mimics. In order to monitor the miRNA-34a replacement, we used a fluorescent nanodiamond particles (FND) system with linked miRNA-34a mimic, which was delivered to PC3 and DU145 prostatic cancer cell lines. We used functionalized nanodiamonds coated with polyethylenimine to transfer miRNA-34a into PC3 and DU145 prostatic cancer cell lines and we measured the zeta-potential of these complexes before using them for in vitro experiments. A replacement of miRNA-34 was observed by monitoring levels of miRNA-34 via real-time PCR. Moreover, our in vitro experiments demonstrated that miRNA-34a replacement, using this FND delivery system, decreased viability and induced apoptosis in prostatic cancer cell lines. Our findings suggest the replacement of oncosuppressor miRNA-34a provides an effective strategy for cancer therapy and the FND-based delivery systems seems to be an excellent strategy for a safe and effective targeting of the tumor.
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Digital nomadism
Petráková, Veronika ; Kubálková, Markéta (advisor) ; Novotná, Blanka (referee)
This bachelor thesis deals with the complex introduction of the new trend of livelihood - digital nomadism and examines the economic aspect of this phenomenon. In the theoretical part, digital nomadism is introduced and explains how digital nomads can earn money and what types of work they perform most often. Furthermore, the specifics of distance learning and the specifics of employment of remote workers are described here. In the practical part is described and evaluated questionnaire survey, which examines the economic situation of Czech digital nomads.
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Production of nitrogen vacancy centers in nanocrystalline diamond thin film for quantum biosensing applications
Jakl Krečmarová, Marie ; Gulka, Michal ; Fekete, Ladislav ; Remeš, Zdeněk ; Petráková, Vladimíra ; Mortet, Vincent ; Nesládek, M.
Due to its excellent properties such as chemical stability and biocompatibility, diamond is an ideal material for bio sensing application. In particular, nitrogen vacancy (NV) centres in diamond are promising candidates for optical bio-sensing application in nanodiamond particles and single crystal diamond by irradiation (electron, proton, neutron, particles) followed by annealing has been recently developer. Production of NV centres in CVD diamond thin film is important for fabrication of new bio sensor.
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