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Vasa vasorum of the coronary arteries in an animal heart model
Patzelt, Matěj ; Stingl, Josef (advisor) ; Laichman, Stanislav (referee) ; Slížová, Dáša (referee)
The vasa vasorum of the coronary arteries is a system of tiny vessels that nourish the wall of the coronary arteries. Recently, the vasa vasorum has been attributed importance in the pathogenesis of atherosclerosis, necrosis of the tunica media, or in excessive healing of the intima after stenting. The vasa vasorum system is described in detail on pathologically altered hearts, however, studies that would deal with a detailed description on a healthy human heart or on its animal model are minimal. The aim of the dissertation was to describe the structure and origin of the vasa vasorum of the coronary arteries on an animal model of the heart of a pig, to describe the history of the term vasa vasorum and possibly to describe secondary findings on the examined samples. A total of 36 healthy pig hearts were used in the study. Part of them was injected with India ink through the ostium of the coronary arteries and subsequently histologically processed, other hearts were directly fixed in toto in formalin and histologically processed, the remaining part of the hearts was injected through the ostium of the coronary arteries with mercox resin and then corrosive casts of the coronary arteries were created. These samples were evaluated using a scanning electron microscope. Histological processing of the...
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Imaging of Soft Tissues in Micro-CT
Patzelt, Matěj ; Rosina, Jozef (advisor) ; Vránová, Jana (referee) ; Fiala, Pavel (referee)
X-ray radiography is a noninvasive imaging technique for a visualization of internal structures of investigated samples. In past years, imaging of biological samples in a micro-CT is gaining in popularity. The disadvantage of this technology is a low ability to display soft tissues like muscles, fat tissue or nerves due to their low intrinsic contrast. The aim of this study was to modify a conventional micro-CT MARS in order to improve the scanning of the soft tissues and to create a protocol for simple and cheap fixation of ex-vivo soft tissues for micro-CT scanning. In the study, a modified micro-CT MARS was used together with a photon-counting detector Timepix Quad with the resolution 30µm. The micro-CT was afterward tested on the phantom and also on the real soft tissues. For scanning soft tissues in the micro-CT, the ethanol fixation method was invented. Hearts and lungs from laboratory mice were either fixated in 97%, 50% ethanol solution or in a series of ascending ethanol concentrations. Images were acquired after 72, 168 and 336 hours. The resulting images were compared among themselves and with the native specimens. This fixation method was also used in scanning healthy mice brains in order to evaluate the contribution of displaying of a brain in a micro-CT in the research of the central...
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